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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Orientia tsutsugamushi (strain Boryong) (Rickettsia tsutsugamushi)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei611 – 6111ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciOTSU357244:GCA5-482-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:OTBS_0468
OrganismiOrientia tsutsugamushi (strain Boryong) (Rickettsia tsutsugamushi)
Taxonomic identifieri357244 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeOrientia
ProteomesiUP000001565: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 838838Leucine--tRNA ligasePRO_0000334786Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi357244.OTBS_0468.

Structurei

3D structure databases

ProteinModelPortaliA5CCT4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi38 – 4811"HIGH" regionAdd
BLAST
Motifi608 – 6125"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

A5CCT4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNLVKIEKKW QKIWEQNEVF KVKEILNKIK RYILPMFPYP SGKAHVGHVR
60 70 80 90 100
NYTICDVIAR FERSQGHNVL HAMGWDAFGL PAENAAMQNN TYPNSWVSSN
110 120 130 140 150
VDVMRQQLKA IGLSYDWSRE IITCSSQYYV HEQRFFLEML KKGLVYQKES
160 170 180 190 200
LVNWDPIDQT VLANEQVING KGWRSGAIIE YRNLKQWFIK ITNYADALLK
210 220 230 240 250
GLDSLKGWPE SVKTMQKKWI GQSTGININF QLKGIEASVK VFSTRPETLF
260 270 280 290 300
GASFIALSYN HNLVQQYVNT TPETQKFIDK CSNVGTSNVN IDKMKIAVLT
310 320 330 340 350
NLKVIHPLNS SIELPVILSN FVLMDYGTGA LFGCPAHDER DHEIAKLLKL
360 370 380 390 400
NIKQVITSTE RNIDVLKEAY VGDGIMINSF HLNGLTTTEA RQKVINELQH
410 420 430 440 450
KNIGQQVTNY KLKDWGISRQ RFWGCPIPII HCKSCGAVPV PYEDLPVILP
460 470 480 490 500
EHGVEFTGKG NPLDNHHSWK YVKCPKCHLD AVRETDTFDT FFESSWYFAR
510 520 530 540 550
FCNPTSDDMV DAKAAKYWLP VDQYIGGIEH AVMHLLYARF ITRVMYDLKY
560 570 580 590 600
IDIQEPFTSL ITQGMVLHRT YQDKSNNWLY PNEVEVDSNG QLRCKADLQY
610 620 630 640 650
VTVGKLEKMS KSKKNVVDLE LVLKLYGADV ARMFVLSDTP PEKDLEWSTE
660 670 680 690 700
GIEGCYKFIQ KLYNFALKLK NINLTDNKID KVLLSKTHKT IKNVTQDIIS
710 720 730 740 750
CRLNKAIARL RELYNLIFKM SELTVQIKES FLILIRLFNP FIPHLSEEIW
760 770 780 790 800
SLLSGRGEML VELPWPKYEE KYIHEEEHIT IAIQINGKLR SLYNCLIDTP
810 820 830
EHDVQSAILK LEQVKKHIGD KEVRKCIFVP NKLINIII
Length:838
Mass (Da):96,655
Last modified:June 12, 2007 - v1
Checksum:iF8757CA6ACA4175C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM494475 Genomic DNA. Translation: CAM79534.1.
RefSeqiWP_011944500.1. NC_009488.1.
YP_001248365.1. NC_009488.1.

Genome annotation databases

EnsemblBacteriaiCAM79534; CAM79534; OTBS_0468.
GeneIDi5218800.
KEGGiots:OTBS_0468.
PATRICi22822144. VBIOriTsu83812_0499.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM494475 Genomic DNA. Translation: CAM79534.1.
RefSeqiWP_011944500.1. NC_009488.1.
YP_001248365.1. NC_009488.1.

3D structure databases

ProteinModelPortaliA5CCT4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi357244.OTBS_0468.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAM79534; CAM79534; OTBS_0468.
GeneIDi5218800.
KEGGiots:OTBS_0468.
PATRICi22822144. VBIOriTsu83812_0499.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciOTSU357244:GCA5-482-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The Orientia tsutsugamushi genome reveals massive proliferation of conjugative type IV secretion system and host-cell interaction genes."
    Cho N.-H., Kim H.-R., Lee J.-H., Kim S.-Y., Kim J., Cha S., Kim S.-Y., Darby A.C., Fuxelius H.-H., Yin J., Kim J.H., Kim J., Lee S.J., Koh Y.-S., Jang W.-J., Park K.-H., Andersson S.G.E., Choi M.-S., Kim I.-S.
    Proc. Natl. Acad. Sci. U.S.A. 104:7981-7986(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Boryong.

Entry informationi

Entry nameiSYL_ORITB
AccessioniPrimary (citable) accession number: A5CCT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 12, 2007
Last modified: February 4, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.