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A5CCT4 (SYL_ORITB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:OTBS_0468
OrganismOrientia tsutsugamushi (strain Boryong) (Rickettsia tsutsugamushi) [Complete proteome] [HAMAP]
Taxonomic identifier357244 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeOrientia

Protein attributes

Sequence length838 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 838838Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334786

Regions

Motif38 – 4811"HIGH" region HAMAP-Rule MF_00049
Motif608 – 6125"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6111ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A5CCT4 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: F8757CA6ACA4175C

FASTA83896,655
        10         20         30         40         50         60 
MNLVKIEKKW QKIWEQNEVF KVKEILNKIK RYILPMFPYP SGKAHVGHVR NYTICDVIAR 

        70         80         90        100        110        120 
FERSQGHNVL HAMGWDAFGL PAENAAMQNN TYPNSWVSSN VDVMRQQLKA IGLSYDWSRE 

       130        140        150        160        170        180 
IITCSSQYYV HEQRFFLEML KKGLVYQKES LVNWDPIDQT VLANEQVING KGWRSGAIIE 

       190        200        210        220        230        240 
YRNLKQWFIK ITNYADALLK GLDSLKGWPE SVKTMQKKWI GQSTGININF QLKGIEASVK 

       250        260        270        280        290        300 
VFSTRPETLF GASFIALSYN HNLVQQYVNT TPETQKFIDK CSNVGTSNVN IDKMKIAVLT 

       310        320        330        340        350        360 
NLKVIHPLNS SIELPVILSN FVLMDYGTGA LFGCPAHDER DHEIAKLLKL NIKQVITSTE 

       370        380        390        400        410        420 
RNIDVLKEAY VGDGIMINSF HLNGLTTTEA RQKVINELQH KNIGQQVTNY KLKDWGISRQ 

       430        440        450        460        470        480 
RFWGCPIPII HCKSCGAVPV PYEDLPVILP EHGVEFTGKG NPLDNHHSWK YVKCPKCHLD 

       490        500        510        520        530        540 
AVRETDTFDT FFESSWYFAR FCNPTSDDMV DAKAAKYWLP VDQYIGGIEH AVMHLLYARF 

       550        560        570        580        590        600 
ITRVMYDLKY IDIQEPFTSL ITQGMVLHRT YQDKSNNWLY PNEVEVDSNG QLRCKADLQY 

       610        620        630        640        650        660 
VTVGKLEKMS KSKKNVVDLE LVLKLYGADV ARMFVLSDTP PEKDLEWSTE GIEGCYKFIQ 

       670        680        690        700        710        720 
KLYNFALKLK NINLTDNKID KVLLSKTHKT IKNVTQDIIS CRLNKAIARL RELYNLIFKM 

       730        740        750        760        770        780 
SELTVQIKES FLILIRLFNP FIPHLSEEIW SLLSGRGEML VELPWPKYEE KYIHEEEHIT 

       790        800        810        820        830 
IAIQINGKLR SLYNCLIDTP EHDVQSAILK LEQVKKHIGD KEVRKCIFVP NKLINIII 

« Hide

References

[1]"The Orientia tsutsugamushi genome reveals massive proliferation of conjugative type IV secretion system and host-cell interaction genes."
Cho N.-H., Kim H.-R., Lee J.-H., Kim S.-Y., Kim J., Cha S., Kim S.-Y., Darby A.C., Fuxelius H.-H., Yin J., Kim J.H., Kim J., Lee S.J., Koh Y.-S., Jang W.-J., Park K.-H., Andersson S.G.E., Choi M.-S., Kim I.-S.
Proc. Natl. Acad. Sci. U.S.A. 104:7981-7986(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Boryong.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM494475 Genomic DNA. Translation: CAM79534.1.
RefSeqYP_001248365.1. NC_009488.1.

3D structure databases

ProteinModelPortalA5CCT4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING357244.OTBS_0468.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAM79534; CAM79534; OTBS_0468.
GeneID5218800.
KEGGots:OTBS_0468.
PATRIC22822144. VBIOriTsu83812_0499.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAPVIHCPK.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycOTSU357244:GCA5-482-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL_ORITB
AccessionPrimary (citable) accession number: A5CCT4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 12, 2007
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries