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A5CCM4

- LIPA_ORITB

UniProt

A5CCM4 - LIPA_ORITB

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Protein

Lipoyl synthase

Gene

lipA

Organism
Orientia tsutsugamushi (strain Boryong) (Rickettsia tsutsugamushi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi49 – 491Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi55 – 551Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi70 – 701Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi74 – 741Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi77 – 771Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciOTSU357244:GCA5-402-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:OTBS_0390
OrganismiOrientia tsutsugamushi (strain Boryong) (Rickettsia tsutsugamushi)
Taxonomic identifieri357244 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeOrientia
ProteomesiUP000001565: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302Lipoyl synthasePRO_0000325282Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi357244.OTBS_0390.

Structurei

3D structure databases

ProteinModelPortaliA5CCM4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiHTICQEA.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A5CCM4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCSDKNITAT ALVRPSWLRV KAPFSDEYQS TNELIKSLKL NTVCKEAACP
60 70 80 90 100
NIGECWSKKH ATVMILGSIC TRACAFCNVS TGKPEQVDEY EPYRLSEAVM
110 120 130 140 150
KLGLKHVVIT SVDRDDLSDG GASHFAKCIT YIRERSPTTS IEVLTPDFLR
160 170 180 190 200
KHEAWKIVAK ARPDVYNHNI ETVPSLYLKV RPGARYYNSL NLLHQVKIFD
210 220 230 240 250
SSIFTKSGIM VGLGETKHEV LQVMDDLRAA EVDFLTIGQY LRPSARHIDV
260 270 280 290 300
GRYVTPDEFD YYAKVARSKG FLMVSASPLT RSSYHAGEHF EKLKQMRLQN

II
Length:302
Mass (Da):34,099
Last modified:June 12, 2007 - v1
Checksum:i0CDBCC6BB5370DF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM494475 Genomic DNA. Translation: CAM79456.1.
RefSeqiYP_001248314.1. NC_009488.1.

Genome annotation databases

EnsemblBacteriaiCAM79456; CAM79456; OTBS_0390.
GeneIDi5218720.
KEGGiots:OTBS_0390.
PATRICi22821972. VBIOriTsu83812_0415.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM494475 Genomic DNA. Translation: CAM79456.1 .
RefSeqi YP_001248314.1. NC_009488.1.

3D structure databases

ProteinModelPortali A5CCM4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 357244.OTBS_0390.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAM79456 ; CAM79456 ; OTBS_0390 .
GeneIDi 5218720.
KEGGi ots:OTBS_0390.
PATRICi 22821972. VBIOriTsu83812_0415.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi HTICQEA.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci OTSU357244:GCA5-402-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The Orientia tsutsugamushi genome reveals massive proliferation of conjugative type IV secretion system and host-cell interaction genes."
    Cho N.-H., Kim H.-R., Lee J.-H., Kim S.-Y., Kim J., Cha S., Kim S.-Y., Darby A.C., Fuxelius H.-H., Yin J., Kim J.H., Kim J., Lee S.J., Koh Y.-S., Jang W.-J., Park K.-H., Andersson S.G.E., Choi M.-S., Kim I.-S.
    Proc. Natl. Acad. Sci. U.S.A. 104:7981-7986(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Boryong.

Entry informationi

Entry nameiLIPA_ORITB
AccessioniPrimary (citable) accession number: A5CCM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 12, 2007
Last modified: November 26, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3