A5CCL4 (EFTU2_ORITB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor Tu 2
Short name=EF-Tu 2

Gene names

Name:	tuf2
Ordered Locus Names:	OTBS_0378

Organism

Orientia tsutsugamushi (strain Boryong) (Rickettsia tsutsugamushi) [Complete proteome] [HAMAP]

Taxonomic identifier

357244 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Orientia › Orientia tsutsugamushi

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis By similarity. HAMAP-Rule MF_00118
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00118
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00118.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
Sequence caution	The sequence CAM79444.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Elongation factor Tu 2 HAMAP-Rule MF_00118		PRO_0000337452
Regions
	Nucleotide binding	18 – 25	8	GTP By similarity
	Nucleotide binding	82 – 86	5	GTP By similarity
	Nucleotide binding	137 – 140	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5CCL4 [UniParc]. Last modified May 20, 2008. Version 2. Checksum: F5BAFC2C4D372A35	FASTA	394	42,860
	10 20 30 40 50 60 MAVAFNRDKP HCNIGTIGHV DHGKTSLATA ITIVSSELSG GAVKVKNYDE IDSAPEERAR 70 80 90 100 110 120 GITIQTAHVE FISKKRHYAL VDCPGHVDYI KNMITGASQT DGLILVVSGV DGVMPQTREH 130 140 150 160 170 180 VLLAKQVGVP SIIVCINKID QADPELLELI EMEVRELLTK YDFPGDTVPI IRCSALKAIN 190 200 210 220 230 240 GDSDAKKGIL ELMDSIDDYI PQPTRVLDQP FLMPIEDVFS ILGRGTVVTG RIERGVIKVG 250 260 270 280 290 300 DEVEIVGLRS TQKTICTGVE MFKKELDQGQ AGDNVGILLR GIKREDVERG QVLAKPGTIT 310 320 330 340 350 360 PHCSFEAEVY VLTKEEGGRH TPFFQNYRPQ FYCRTTDVTG EIALLSGKEM VMPGDHATLS 370 380 390 VNLVAPIAMD QGLSFAIREG GKTIGAGKVS KIIK

References

[1]

"The Orientia tsutsugamushi genome reveals massive proliferation of conjugative type IV secretion system and host-cell interaction genes." Cho N.-H., Kim H.-R., Lee J.-H., Kim S.-Y., Kim J., Cha S., Kim S.-Y., Darby A.C., Fuxelius H.-H., Yin J., Kim J.H., Kim J., Lee S.J., Koh Y.-S., Jang W.-J., Park K.-H., Andersson S.G.E., Choi M.-S., Kim I.-S. Proc. Natl. Acad. Sci. U.S.A. 104:7981-7986(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Boryong.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM494475 Genomic DNA. Translation: CAM79444.1. Different initiation.
RefSeq	YP_001248306.2. NC_009488.1.
3D structure databases
ProteinModelPortal	A5CCL4.
SMR	A5CCL4. Positions 5-393.
ModBase	Search...
Protein-protein interaction databases
STRING	357244.OTBS_0378.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAM79444; CAM79444; OTBS_0378.
GeneID	5218706.
KEGG	ots:OTBS_0378.
PATRIC	22821950. VBIOriTsu83812_0406.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0050.
HOGENOM	HOG000229290.
KO	K02358.
ProtClustDB	PRK00049.
Enzyme and pathway databases
BioCyc	OTSU357244:GCA5-388-MONOMER.
Family and domain databases
HAMAP	MF_00118_B. EF_Tu_B.
InterPro	IPR000795. EF_GTP-bd_dom. IPR027417. P-loop_NTPase. IPR005225. Small_GTP-bd_dom. IPR009001. Transl_elong_EF1A/Init_IF2_C. IPR004161. Transl_elong_EFTu/EF1A_2. IPR004541. Transl_elong_EFTu/EF1A_bac/org. IPR004160. Transl_elong_EFTu/EF1A_C. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF03143. GTP_EFTU_D3. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SUPFAM	SSF50465. Elong_init_C. 1 hit. SSF52540. SSF52540. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00485. EF-Tu. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFTU2_ORITB

Accession

Primary (citable) accession number: A5CCL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	May 29, 2013
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program