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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Orientia tsutsugamushi (strain Boryong) (Rickettsia tsutsugamushi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi492 – 4921MagnesiumUniRule annotation
Metal bindingi498 – 4981MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciOTSU357244:GCA5-165-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:OTBS_0162
OrganismiOrientia tsutsugamushi (strain Boryong) (Rickettsia tsutsugamushi)
Taxonomic identifieri357244 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeOrientia
ProteomesiUP000001565: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 740740Polyribonucleotide nucleotidyltransferasePRO_0000329746Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi357244.OTBS_0162.

Structurei

3D structure databases

ProteinModelPortaliA5CC62.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini559 – 61860KHUniRule annotationAdd
BLAST
Domaini628 – 69669S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5CC62-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFNEILKKVD WHGNVLSLST GKIARNADGA VLASMGNTSV LCTVVFDKNT
60 70 80 90 100
KKDMDFFPLG VYYREMAYAA GKIPGGFIKK EGKFSEYEVL VSRLIDRSIR
110 120 130 140 150
PLFDSNFRND TQIICTVMSY DPRYSPDILA IIGSSAALAI SGIPIVKPIG
160 170 180 190 200
AARVGIVNDE FILNPVIHDN TGVNELDLVV AATFDSVTMI EAQACEIDEE
210 220 230 240 250
KMLAAIEFGY KSLKPVINAI EEIKSSIRKD IFEVTARPHL RYNDEILKHF
260 270 280 290 300
SSDIKSALLL QTKNERNQQL QLIQQKVIDY FSSKANSEAN DGDDILNIEK
310 320 330 340 350
ALDDAKSKIF RDLVLQNKTR IGNRAVDEIR PIICEAGLFN TVHGSALFTR
360 370 380 390 400
GDTQSLATIT LGSSTDEQIV EQLNKCERQN FLLDYIFLPY SVGEISPLRA
410 420 430 440 450
ASRREIGHGW LAKKAIQLVI PSKDVFPYTI RIVSEITQSD GSSSMATVCS
460 470 480 490 500
ASLSLMEAGV PIKTHVAGIA MGLVLGEGNK FEILSDISGC EDHLGDMDFK
510 520 530 540 550
VASTKNGITA LQLDIKVQGI NLSMIESTFR QAKIGINHIL NVMNNTISCP
560 570 580 590 600
KSELSTYAPM VQTLEIQKEK IRDVIGLGGK VIKELCKTFD VEIDISENGE
610 620 630 640 650
VKVWGNVGEN VKKAVQSIEN IVFVPQIGDI FDGEVVKVIE SGAFIKYVTG
660 670 680 690 700
RDGFVHISEI NDTHIKDINA HVKLGDKVKV KIIGIDHKNR VKLTLRTDKE
710 720 730 740
HCKNKNEQYN DITTTTGGVK KKIKIAPKEA AVISNRKYFD
Length:740
Mass (Da):81,783
Last modified:June 12, 2007 - v1
Checksum:iC75060B6EC8AA41D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM494475 Genomic DNA. Translation: CAM79228.1.
RefSeqiYP_001248172.1. NC_009488.1.

Genome annotation databases

EnsemblBacteriaiCAM79228; CAM79228; OTBS_0162.
GeneIDi5218483.
KEGGiots:OTBS_0162.
PATRICi22821490. VBIOriTsu83812_0183.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM494475 Genomic DNA. Translation: CAM79228.1.
RefSeqiYP_001248172.1. NC_009488.1.

3D structure databases

ProteinModelPortaliA5CC62.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi357244.OTBS_0162.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAM79228; CAM79228; OTBS_0162.
GeneIDi5218483.
KEGGiots:OTBS_0162.
PATRICi22821490. VBIOriTsu83812_0183.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciOTSU357244:GCA5-165-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The Orientia tsutsugamushi genome reveals massive proliferation of conjugative type IV secretion system and host-cell interaction genes."
    Cho N.-H., Kim H.-R., Lee J.-H., Kim S.-Y., Kim J., Cha S., Kim S.-Y., Darby A.C., Fuxelius H.-H., Yin J., Kim J.H., Kim J., Lee S.J., Koh Y.-S., Jang W.-J., Park K.-H., Andersson S.G.E., Choi M.-S., Kim I.-S.
    Proc. Natl. Acad. Sci. U.S.A. 104:7981-7986(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Boryong.

Entry informationi

Entry nameiPNP_ORITB
AccessioniPrimary (citable) accession number: A5CC62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 12, 2007
Last modified: January 7, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.