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A5CB81

- LIAS_VITVI

UniProt

A5CB81 - LIAS_VITVI

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Protein

Lipoyl synthase, mitochondrial

Gene

LIP1

Organism
Vitis vinifera (Grape)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi115 – 1151Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi120 – 1201Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi126 – 1261Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi146 – 1461Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi150 – 1501Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi153 – 1531Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. aromatic amino acid family biosynthetic process Source: EnsemblPlants/Gramene
  2. cell differentiation Source: EnsemblPlants/Gramene
  3. chlorophyll biosynthetic process Source: EnsemblPlants/Gramene
  4. cysteine biosynthetic process Source: EnsemblPlants/Gramene
  5. glycine catabolic process Source: EnsemblPlants/Gramene
  6. isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: EnsemblPlants/Gramene
  7. jasmonic acid biosynthetic process Source: EnsemblPlants/Gramene
  8. leaf morphogenesis Source: EnsemblPlants/Gramene
  9. nucleotide metabolic process Source: EnsemblPlants/Gramene
  10. oxidoreduction coenzyme metabolic process Source: EnsemblPlants/Gramene
  11. positive regulation of transcription, DNA-templated Source: EnsemblPlants/Gramene
  12. protein lipoylation Source: UniProtKB-HAMAP
  13. secondary metabolic process Source: EnsemblPlants/Gramene
  14. unsaturated fatty acid biosynthetic process Source: EnsemblPlants/Gramene
  15. vitamin metabolic process Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
Name:LIP1UniRule annotation
ORF Names:VITISV_021769
OrganismiVitis vinifera (Grape)
Taxonomic identifieri29760 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsVitalesVitaceaeVitis

Subcellular locationi

Mitochondrion UniRule annotation

GO - Cellular componenti

  1. mitochondrial matrix Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 393Lipoyl synthase, mitochondrialPRO_0000398857
Transit peptidei1 – ?MitochondrionUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi29760.GSVIVG00003955001.

Structurei

3D structure databases

ProteinModelPortaliA5CB81.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
KOiK03644.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
MF_03128. Lipoyl_synth_plantM.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027527. Lipoyl_synth_mt.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A5CB81-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYTRLSSLAL RHHRHNHNNS VLLLSSLISL RHCSSPPTNP EPPQTLASLR
60 70 80 90 100
HRLAVESPSL SDFVRLQTSD DYSVEVGTKK KPLSKPKWMK ESIPGGAKYT
110 120 130 140 150
QIKKKLRQLN LHTVCEEARC PNMGECWSGG ETGTATATIM ILGDTCTRGC
160 170 180 190 200
RFCNVKTSRT PPPPDPDEPS KVAEAIASWG LDYVVITSVD RDDLPDQGSG
210 220 230 240 250
HFAETVQKLK ILKPNMLIEA LVPDFRGDPG CVEKVSKSGL DVFAHNIETV
260 270 280 290 300
EELQSAVRDH RANFKQSLEV LKLAKEYADA GTLTKTSIML GCGETPDQVV
310 320 330 340 350
RTMEKVRAAG VDVMTFGQYM RPSKRHMPVS EYITPEAFEK YRILGMDMGF
360 370 380 390
RYVASGPMVR SSYKAGEFYI KSMIDADRAM SWASSSPSPL PAA
Length:393
Mass (Da):43,477
Last modified:June 12, 2007 - v1
Checksum:i18F30ED79ECD49A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM488835 Genomic DNA. Translation: CAN73265.1.
RefSeqiXP_002266132.2. XM_002266096.2.
UniGeneiVvi.610.

Genome annotation databases

GeneIDi100251365.
KEGGivvi:100251365.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM488835 Genomic DNA. Translation: CAN73265.1 .
RefSeqi XP_002266132.2. XM_002266096.2.
UniGenei Vvi.610.

3D structure databases

ProteinModelPortali A5CB81.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 29760.GSVIVG00003955001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100251365.
KEGGi vvi:100251365.

Phylogenomic databases

eggNOGi COG0320.
KOi K03644.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
MF_03128. Lipoyl_synth_plantM.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027527. Lipoyl_synth_mt.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A high quality draft consensus sequence of the genome of a heterozygous grapevine variety."
    Velasco R., Zharkikh A., Troggio M., Cartwright D.A., Cestaro A., Pruss D., Pindo M., FitzGerald L.M., Vezzulli S., Reid J., Malacarne G., Iliev D., Coppola G., Wardell B., Micheletti D., Macalma T., Facci M., Mitchell J.T.
    , Perazzolli M., Eldredge G., Gatto P., Oyzerski R., Moretto M., Gutin N., Stefanini M., Chen Y., Segala C., Davenport C., Dematte L., Mraz A., Battilana J., Stormo K., Costa F., Tao Q., Si-Ammour A., Harkins T., Lackey A., Perbost C., Taillon B., Stella A., Solovyev V., Fawcett J.A., Sterck L., Vandepoele K., Grando S.M., Toppo S., Moser C., Lanchbury J., Bogden R., Skolnick M., Sgaramella V., Bhatnagar S.K., Fontana P., Gutin A., Van de Peer Y., Salamini F., Viola R.
    PLoS ONE 2:E1326-E1326(2007)
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Pinot noir.

Entry informationi

Entry nameiLIAS_VITVI
AccessioniPrimary (citable) accession number: A5CB81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: June 12, 2007
Last modified: November 26, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3