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A5CB81

- LIAS_VITVI

UniProt

A5CB81 - LIAS_VITVI

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Protein

Lipoyl synthase, mitochondrial

Gene
LIP1, VITISV_021769
Organism
Vitis vinifera (Grape)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi115 – 1151Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi120 – 1201Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi126 – 1261Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi146 – 1461Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi150 – 1501Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi153 – 1531Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
Name:LIP1
ORF Names:VITISV_021769
OrganismiVitis vinifera (Grape)
Taxonomic identifieri29760 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsVitalesVitaceaeVitis

Subcellular locationi

Mitochondrion By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrial matrix Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 393Lipoyl synthase, mitochondrialUniRule annotationPRO_0000398857
Transit peptidei1 – ?Mitochondrion Reviewed prediction

Interactioni

Protein-protein interaction databases

STRINGi29760.GSVIVG00003955001.

Structurei

3D structure databases

ProteinModelPortaliA5CB81.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
KOiK03644.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
MF_03128. Lipoyl_synth_plantM.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027527. Lipoyl_synth_mt.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A5CB81-1 [UniParc]FASTAAdd to Basket

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MYTRLSSLAL RHHRHNHNNS VLLLSSLISL RHCSSPPTNP EPPQTLASLR    50
HRLAVESPSL SDFVRLQTSD DYSVEVGTKK KPLSKPKWMK ESIPGGAKYT 100
QIKKKLRQLN LHTVCEEARC PNMGECWSGG ETGTATATIM ILGDTCTRGC 150
RFCNVKTSRT PPPPDPDEPS KVAEAIASWG LDYVVITSVD RDDLPDQGSG 200
HFAETVQKLK ILKPNMLIEA LVPDFRGDPG CVEKVSKSGL DVFAHNIETV 250
EELQSAVRDH RANFKQSLEV LKLAKEYADA GTLTKTSIML GCGETPDQVV 300
RTMEKVRAAG VDVMTFGQYM RPSKRHMPVS EYITPEAFEK YRILGMDMGF 350
RYVASGPMVR SSYKAGEFYI KSMIDADRAM SWASSSPSPL PAA 393
Length:393
Mass (Da):43,477
Last modified:June 12, 2007 - v1
Checksum:i18F30ED79ECD49A3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM488835 Genomic DNA. Translation: CAN73265.1.
RefSeqiXP_002266132.2. XM_002266096.2.
UniGeneiVvi.610.

Genome annotation databases

GeneIDi100251365.
KEGGivvi:100251365.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM488835 Genomic DNA. Translation: CAN73265.1 .
RefSeqi XP_002266132.2. XM_002266096.2.
UniGenei Vvi.610.

3D structure databases

ProteinModelPortali A5CB81.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 29760.GSVIVG00003955001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100251365.
KEGGi vvi:100251365.

Phylogenomic databases

eggNOGi COG0320.
KOi K03644.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
MF_03128. Lipoyl_synth_plantM.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027527. Lipoyl_synth_mt.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A high quality draft consensus sequence of the genome of a heterozygous grapevine variety."
    Velasco R., Zharkikh A., Troggio M., Cartwright D.A., Cestaro A., Pruss D., Pindo M., FitzGerald L.M., Vezzulli S., Reid J., Malacarne G., Iliev D., Coppola G., Wardell B., Micheletti D., Macalma T., Facci M., Mitchell J.T.
    , Perazzolli M., Eldredge G., Gatto P., Oyzerski R., Moretto M., Gutin N., Stefanini M., Chen Y., Segala C., Davenport C., Dematte L., Mraz A., Battilana J., Stormo K., Costa F., Tao Q., Si-Ammour A., Harkins T., Lackey A., Perbost C., Taillon B., Stella A., Solovyev V., Fawcett J.A., Sterck L., Vandepoele K., Grando S.M., Toppo S., Moser C., Lanchbury J., Bogden R., Skolnick M., Sgaramella V., Bhatnagar S.K., Fontana P., Gutin A., Van de Peer Y., Salamini F., Viola R.
    PLoS ONE 2:E1326-E1326(2007)
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Pinot noir.

Entry informationi

Entry nameiLIAS_VITVI
AccessioniPrimary (citable) accession number: A5CB81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: June 12, 2007
Last modified: April 16, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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