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Protein

Aspartate aminotransferase, cytoplasmic

Gene

GOT1

Organism
Pan troglodytes (Chimpanzee)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain (By similarity).By similarity

Miscellaneous

In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.

Catalytic activityi

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
L-cysteine + 2-oxoglutarate = mercaptopyruvate + L-glutamate.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39Aspartate; via amide nitrogenBy similarity1
Binding sitei141AspartateBy similarity1
Binding sitei195AspartateBy similarity1
Binding sitei387AspartateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase
Biological processAmino-acid biosynthesis
LigandPyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate aminotransferase, cytoplasmic (EC:2.6.1.1, EC:2.6.1.3)
Short name:
cAspAT
Alternative name(s):
Cysteine aminotransferase, cytoplasmic
Cysteine transaminase, cytoplasmic
Short name:
cCAT
Glutamate oxaloacetate transaminase 1
Transaminase A
Gene namesi
Name:GOT1
OrganismiPan troglodytes (Chimpanzee)
Taxonomic identifieri9598 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePan
Proteomesi
  • UP000002277 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002975461 – 413Aspartate aminotransferase, cytoplasmicAdd BLAST413

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei149PhosphoserineBy similarity1
Modified residuei259N6-(pyridoxal phosphate)lysineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiA5A6K8.
PRIDEiA5A6K8.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9598.ENSPTRP00000004968.

Structurei

3D structure databases

ProteinModelPortaliA5A6K8.
SMRiA5A6K8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1411. Eukaryota.
COG1448. LUCA.
HOGENOMiHOG000185898.
HOVERGENiHBG000951.
InParanoidiA5A6K8.
KOiK14454.

Family and domain databases

Gene3Di3.90.1150.10. 2 hits.
InterProiView protein in InterPro
IPR004839. Aminotransferase_I/II.
IPR000796. Asp_trans.
IPR004838. NHTrfase_class1_PyrdxlP-BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015422. PyrdxlP-dep_Trfase_sub2.
PANTHERiPTHR11879. PTHR11879. 1 hit.
PfamiView protein in Pfam
PF00155. Aminotran_1_2. 1 hit.
PRINTSiPR00799. TRANSAMINASE.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiView protein in PROSITE
PS00105. AA_TRANSFER_CLASS_1. 1 hit.

Sequencei

Sequence statusi: Complete.

A5A6K8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV
60 70 80 90 100
LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR
110 120 130 140 150
VGGVQSLGGT GALRIGADFL ARWYNGTNNK NTPVYVSSPT WENHNAVFSA
160 170 180 190 200
AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIVVLH ACAHNPTGID
210 220 230 240 250
PTPEQWKQIA SVMKHRFLFP FFDSAYQGFA SGNLERDAWA IRYFVSEGFE
260 270 280 290 300
FFCAQSFSKN FGLYNERVGN LTVVGKEPES ILQVLSQMEK IVRITWSNPP
310 320 330 340 350
AQGARIVAST LSNPELFEEW TGNVKTMADR ILSMRSELRA RLEALKTPGT
360 370 380 390 400
WNHITDQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINV SGLTTKNLDY
410
VATSIHEAVT KIQ
Length:413
Mass (Da):46,233
Last modified:June 12, 2007 - v1
Checksum:i6CFB38EA09540219
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB222136 mRNA. Translation: BAF62381.1.
RefSeqiNP_001092011.1. NM_001098541.2.
UniGeneiPtr.165.

Genome annotation databases

GeneIDi450664.
KEGGiptr:450664.

Similar proteinsi

Entry informationi

Entry nameiAATC_PANTR
AccessioniPrimary (citable) accession number: A5A6K8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: June 12, 2007
Last modified: August 30, 2017
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families