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Protein

Small toxic protein TisB

Gene

tisB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

A toxic protein; overexpression causes cessation of growth. Overexpression induces stress-response, a number of membrane protein genes, and leads to cell death. Part of the programmed response to DNA damage; damage leads to increased accumulation of the protein which slows or stops bacterial growth, probably allowing DNA repair before cells continue to grow.3 Publications

GO - Molecular functioni

  • anion channel activity Source: EcoCyc

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • dormancy process Source: EcoCyc
  • SOS response Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-1922.

Protein family/group databases

TCDBi1.C.103.1.1. the pore-forming toxin, tisb (tisb) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Small toxic protein TisB
Alternative name(s):
LexA-regulated protein TisB
Gene namesi
Name:tisB
Synonyms:ysdB
Ordered Locus Names:b4618, JW3649
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14402. tisB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei6 – 2823HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Competition experiments between isogenic strains with or without the tisB/istR-1 region were performed. In the presence of DNA-damaging agents deletion strains were disadvantaged and were almost extinct by 4 days.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2929Small toxic protein TisBPRO_0000311834Add
BLAST

Proteomic databases

PaxDbiA5A627.

Expressioni

Inductioni

Part of the SOS-response regulon, controlled by LexA. The sRNA istR-1 inhibits toxicity by sequestering the standby ribosome binding site for tisB; as the levels of istR-1 decrease this site opens and ribosomes are able to bind to initiate translation further downstream. A type I toxin antitoxin (TA) system, where expression of the proteinaceous toxin is controlled by an antisense sRNA.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi511145.b4618.

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK19148.

Family and domain databases

InterProiIPR025211. TisB_toxin.
[Graphical view]
PfamiPF13939. TisB_toxin. 1 hit.
[Graphical view]
TIGRFAMsiTIGR04459. TisB_tox. 1 hit.

Sequencei

Sequence statusi: Complete.

A5A627-1 [UniParc]FASTAAdd to basket

« Hide

        10         20 
MNLVDIAILI LKLIVAALQL LDAVLKYLK
Length:29
Mass (Da):3,222
Last modified:May 29, 2007 - v1
Checksum:i6F3B7FE3D9FEFC80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: ABP93456.1.
AP009048 Genomic DNA. No translation available.
RefSeqiWP_001054909.1. NZ_LN832404.1.
YP_001165331.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiABP93456; ABP93456; b4618.
GeneIDi5061527.
KEGGieco:b4618.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: ABP93456.1.
AP009048 Genomic DNA. No translation available.
RefSeqiWP_001054909.1. NZ_LN832404.1.
YP_001165331.1. NC_000913.3.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi511145.b4618.

Protein family/group databases

TCDBi1.C.103.1.1. the pore-forming toxin, tisb (tisb) family.

Proteomic databases

PaxDbiA5A627.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP93456; ABP93456; b4618.
GeneIDi5061527.
KEGGieco:b4618.

Organism-specific databases

EcoGeneiEG14402. tisB.

Phylogenomic databases

KOiK19148.

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-1922.

Miscellaneous databases

PROiA5A627.

Family and domain databases

InterProiIPR025211. TisB_toxin.
[Graphical view]
PfamiPF13939. TisB_toxin. 1 hit.
[Graphical view]
TIGRFAMsiTIGR04459. TisB_tox. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTISB_ECOLI
AccessioniPrimary (citable) accession number: A5A627
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: May 29, 2007
Last modified: July 6, 2016
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Originally tisA and tisB were fused into one ORF by frameshift reconstruction. TisA is probably a pseudogene.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.