A5A3E0 (POTEF_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
POTE ankyrin domain family member F

Alternative name(s):
ANKRD26-like family C member 1B
Chimeric POTE-actin protein

Gene names

Name:	POTEF
Synonyms:	A26C1B

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1075 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cytoplasm › cell cortex. Note: Colocalizes with actin filaments. Ref.1
Tissue specificity	Expressed in breast cancer cell lines (at protein level). Ref.1
Miscellaneous	Results from the insertion of a beta-actin fragment at the C-terminus in the POTEE paralog gene leading to the formation of a new functional chimeric protein. This insertion occured before the divergence of the Old World monkeys and apes.
Sequence similarities	In the N-terminal section; belongs to the POTE family. In the C-terminal section; belongs to the actin family. Contains 5 ANK repeats.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	ANK repeat Coiled coil Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cell cortex Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1075	1075	POTE ankyrin domain family member F		PRO_0000307859
Regions
	Repeat	172 – 201	30	ANK 1
	Repeat	205 – 234	30	ANK 2
	Repeat	238 – 267	30	ANK 3
	Repeat	271 – 300	30	ANK 4
	Repeat	304 – 333	30	ANK 5
	Region	702 – 1075	374	Actin-like
	Coiled coil	399 – 435	37	Potential
	Coiled coil	642 – 698	57	Potential
Amino acid modifications
	Modified residue	1006	1	Phosphotyrosine Ref.3
Experimental info
	Sequence conflict	4	1	E → G in ABP57734. Ref.1
	Sequence conflict	387	1	S → L in ABP57734. Ref.1
	Sequence conflict	930	1	V → A in ABP57734. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	A5A3E0 [UniParc]. Last modified November 25, 2008. Version 2. Checksum: 8833FF435D1B6902	FASTA	1,075	121,445
	10 20 30 40 50 60 MVVEVDSMPA ASSVKKPFGL RSKMGKWCCR CFPCCRESGK SNVGTSGDHD DSAMKTLRSK 70 80 90 100 110 120 MGKWCRHCFP CCRGSGKSNV GASGDHDDSA MKTLRNKMGK WCCHCFPCCR GSSKSKVGAW 130 140 150 160 170 180 GDYDDSAFME PRYHVRGEDL DKLHRAAWWG KVPRKDLIVM LRDTDVNKQD KQKRTALHLA 190 200 210 220 230 240 SANGNSEVVK LLLDRRCQLN VLDNKKRTAL IKAVQCQEDE CALMLLEHGT DPNIPDEYGN 250 260 270 280 290 300 TTLHYAIYNE DKLMAKALLL YGADIESKNK HGLTPLLLGV HEQKQQVVKF LIKKKANLNA 310 320 330 340 350 360 LDRYGRTALI LAVCCGSASI VSLLLEQNID VSSQDLSGQT AREYAVSSHH HVICQLLSDY 370 380 390 400 410 420 KEKQMLKISS ENSNPEQDLK LTSEEESQRF KGSENSQPEK MSQEPEINKD GDREVEEEMK 430 440 450 460 470 480 KHESNNVGLL ENLTNGVTAG NGDNGLIPQR KSRTPENQQF PDNESEEYHR ICELLSDYKE 490 500 510 520 530 540 KQMPKYSSEN SNPEQDLKLT SEEESQRLKG SENGQPEKRS QEPEINKDGD RELENFMAIE 550 560 570 580 590 600 EMKKHRSTHV GFPENLTNGA TAGNGDDGLI PPRKSRTPES QQFPDTENEE YHSDEQNDTQ 610 620 630 640 650 660 KQFCEEQNTG ILHDEILIHE EKQIEVVEKM NSELSLSCKK EKDILHENST LREEIAMLRL 670 680 690 700 710 720 ELDTMKHQSQ LREKKYLEDI ESVKKRNDNL LKALQLNELT MDDDTAVLVI DNGSGMCKAG 730 740 750 760 770 780 FAGDDAPRAV FPSIVGRPRQ QGMMGGMHQK ESYVGKEAQS KRGILTLKYP MEHGIITNWD 790 800 810 820 830 840 DMEKIWHHTF YNELRVAPEE HPVLLTEATL NPKANREKMT QIMFETFNTP AMYVAIQAVL 850 860 870 880 890 900 SLYTSGRTTG IVMDSGDGVT HTVPIYEGNA LPHATLRLDL AGRELPDYLM KILTEHGYRF 910 920 930 940 950 960 TTMAEREIVR DIKEKLCYVA LDFEQEMATV ASSSSLEKSY ELPDGQVITI GNERFRCPEA 970 980 990 1000 1010 1020 LFQPCFLGME SCGIHETTFN SIMKSDVDIR KDLYTNTVLS GGTTMYPGMA HRMQKEIAAL 1030 1040 1050 1060 1070 APSMMKIRII APPKRKYSVW VGGSILASLS TFQQMWISKQ EYDESGPSIV HRKCL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evolution and expression of chimeric POTE-actin genes in the human genome." Lee Y., Ise T., Ha D., Saint Fleur A., Hahn Y., Liu X.-F., Nagata S., Lee B., Bera T.K., Pastan I. Proc. Natl. Acad. Sci. U.S.A. 103:17885-17890(2006) [PubMed: 17101985] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Mammary cancer.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"An extensive survey of tyrosine phosphorylation revealing new sites in human mammary epithelial cells." Heibeck T.H., Ding S.-J., Opresko L.K., Zhao R., Schepmoes A.A., Yang F., Tolmachev A.V., Monroe M.E., Camp D.G. II, Smith R.D., Wiley H.S., Qian W.-J. J. Proteome Res. 8:3852-3861(2009) [PubMed: 19534553] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1006, MASS SPECTROMETRY. Tissue: Mammary epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EF523384 mRNA. Translation: ABP57734.1. AC018804 Genomic DNA. No translation available. AC018865 Genomic DNA. No translation available.
IPI	IPI00739539.
RefSeq	NP_001093241.1. NM_001099771.2.
UniGene	Hs.580547.
3D structure databases
ProteinModelPortal	A5A3E0.
SMR	A5A3E0. Positions 138-364, 702-1075.
ModBase	Search...
Protein-protein interaction databases
STRING	A5A3E0.
PTM databases
PhosphoSite	A5A3E0.
Proteomic databases
PeptideAtlas	A5A3E0.
PRIDE	A5A3E0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000357462; ENSP00000350052; ENSG00000196604. ENST00000409914; ENSP00000386786; ENSG00000196604.
GeneID	728378.
KEGG	hsa:728378.
Organism-specific databases
CTD	728378.
GeneCards	GC02M130831.
H-InvDB	HIX0030053.
HGNC	HGNC:33905. POTEF.
neXtProt	NX_A5A3E0.
PharmGKB	PA164724818.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG17475.
HOGENOM	HBG506345.
InParanoid	A5A3E0.
OMA	CRESGKS.
OrthoDB	EOG4SN1NB.
PhylomeDB	A5A3E0.
Gene expression databases
Bgee	A5A3E0.
CleanEx	HS_POTEF.
Genevestigator	A5A3E0.
Family and domain databases
InterPro	IPR004000. Actin-like. IPR020902. Actin/actin-like_CS. IPR004001. Actin_CS. IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit.
Pfam	PF00022. Actin. 1 hit. PF12796. Ank_2. 2 hits. [Graphical view]
PRINTS	PR00190. ACTIN.
SMART	SM00268. ACTIN. 1 hit. SM00248. ANK. 6 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit.
PROSITE	PS00432. ACTINS_2. 1 hit. PS01132. ACTINS_ACT_LIKE. 1 hit. PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POTEF_HUMAN

Accession

Primary (citable) accession number: A5A3E0
Secondary accession number(s): A6NC34

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	November 25, 2008
Last modified:	December 14, 2011
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.