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Protein

Adenosylhomocysteinase

Gene

ahcY

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine.By similarity

Catalytic activityi

S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine.

Cofactori

NAD+By similarityNote: Binds 1 NAD+ per subunit.By similarity

Pathwayi: L-homocysteine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine.
Proteins known to be involved in this subpathway in this organism are:
  1. Adenosylhomocysteinase (ahcY)
This subpathway is part of the pathway L-homocysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine, the pathway L-homocysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64SubstrateBy similarity1
Binding sitei147SubstrateBy similarity1
Binding sitei209SubstrateBy similarity1
Binding sitei239SubstrateBy similarity1
Binding sitei243SubstrateBy similarity1
Binding sitei244NADBy similarity1
Binding sitei296NADBy similarity1
Binding sitei331NADBy similarity1
Binding sitei399NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi210 – 212NADBy similarity3
Nucleotide bindingi273 – 278NADBy similarity6
Nucleotide bindingi352 – 354NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processOne-carbon metabolism
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00314; UER00076.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosylhomocysteinase (EC:3.3.1.1)
Alternative name(s):
S-adenosyl-L-homocysteine hydrolase
Short name:
AdoHcyase
Gene namesi
Name:ahcY
Ordered Locus Names:MSMEG_1843, MSMEI_1801
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

A4ZHR8:

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003961381 – 485AdenosylhomocysteinaseAdd BLAST485

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki464Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliA4ZHR8.
SMRiA4ZHR8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the adenosylhomocysteinase family.Curated

Phylogenomic databases

KOiK01251.

Family and domain databases

CDDicd00401. SAHH. 1 hit.
HAMAPiMF_00563. AdoHcyase. 1 hit.
InterProiView protein in InterPro
IPR034373. Adenosylhomocysteinase.
IPR000043. Adenosylhomocysteinase-like.
IPR015878. Ado_hCys_hydrolase_NAD-bd.
IPR036291. NAD(P)-bd_dom_sf.
IPR020082. S-Ado-L-homoCys_hydrolase_CS.
PANTHERiPTHR23420. PTHR23420. 1 hit.
PfamiView protein in Pfam
PF05221. AdoHcyase. 1 hit.
PF00670. AdoHcyase_NAD. 1 hit.
PIRSFiPIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMARTiView protein in SMART
SM00996. AdoHcyase. 1 hit.
SM00997. AdoHcyase_NAD. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00936. ahcY. 1 hit.
PROSITEiView protein in PROSITE
PS00738. ADOHCYASE_1. 1 hit.
PS00739. ADOHCYASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

A4ZHR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTELKADVRN GIDYKVADLS LADFGRKEIR LAEHEMPGLM ALRREYHDVQ
60 70 80 90 100
PLKGARISGS LHMTVQTAVL IETLVSLGAE VRWASCNIFS TQDHAAAAVV
110 120 130 140 150
VGPNGTPEEP KGVSVFAWKG ETLEEYWWAA EQMLTWDGEP ANMILDDGGD
160 170 180 190 200
ATMMVLRGAQ YEKAGVVPPA EDDDPAEWKV FLGVLRERFE QDKTKWTKIA
210 220 230 240 250
ESVKGVTEET TTGVLRLYQF AAAGELAFPA INVNDSVTKS KFDNKYGTRH
260 270 280 290 300
SLIDGINRGT DVLIGGKKVL ICGYGDVGKG CAESLAGQGA RVSVTEIDPI
310 320 330 340 350
NALQALMDGF DVRTVEEAIG EADIVITATG NKDIITLDHM KAMKNQAILG
360 370 380 390 400
NIGHFDNEID MAALERSGAK KINIKPQVDQ WIFDDGKSII VLSEGRLLNL
410 420 430 440 450
GNATGHPSFV MSNSFSNQVI AQIELWTKND EYDNEVYRLA KHLDEKVARI
460 470 480
HVEALGGTLT KLSKDQAEYI GVDVEGPYKP EHYRY
Length:485
Mass (Da):53,270
Last modified:May 29, 2007 - v1
Checksum:iC30CC1B0621B4628
GO

Sequence cautioni

A4ZHR8: The sequence CP000480 differs from that shown. Reason: Frameshift at position 419.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ866847 Genomic DNA. Translation: ABJ96308.1.
CP000480 Genomic DNA. No translation available.
CP001663 Genomic DNA. Translation: AFP38273.1.
RefSeqiWP_003893235.1. NZ_CP009494.1.

Genome annotation databases

EnsemblBacteriaiAFP38273; AFP38273; MSMEI_1801.
KEGGimsb:LJ00_09190.
msg:MSMEI_1801.
PATRICifig|246196.56.peg.1853.

Similar proteinsi

Entry informationi

Entry nameiSAHH_MYCS2
AccessioniPrimary (citable) accession number: A4ZHR8
Secondary accession number(s): I7FHI1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: May 29, 2007
Last modified: October 25, 2017
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families