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A4ZCW8

- RDRP_ROTHW

UniProt

A4ZCW8 - RDRP_ROTHW

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Protein

RNA-directed RNA polymerase

Gene
N/A
Organism
Rotavirus A (strain Human/United States/Wa/1974 G1-P1A[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

RNA-directed RNA polymerase that is involved in both transcription and genome replication. Together with VP3 capping enzyme, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. One copy of each of the viral (+)RNAs is also recruited during core assembly, together with newly synthesized polymerase complexes and VP2. The polymerase of these novo-formed particles catalyzes the synthesis of complementary minus-strands leading to dsRNA formation. To do so, the polymerase specifically recognizes and binds 4 bases 5'-UGUG-3' in the conserved 3'-sequence of plus-strand RNA templates. VP2 presumably activates the autoinhibited VP1-RNA complex to coordinate packaging and genome replication. Once dsRNA synthesis is complete, the polymerase switches to the transcriptional mode, thus providing secondary transcription (By similarity).PROSITE-ProRule annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

Cofactori

Mg2+Curated

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. transcription, DNA-templated Source: InterPro
  2. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

Magnesium, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase (EC:2.7.7.48)
Alternative name(s):
Protein VP1
OrganismiRotavirus A (strain Human/United States/Wa/1974 G1-P1A[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A)
Taxonomic identifieri10962 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000006581: Genome

Subcellular locationi

Virion Curated
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging (By similarity).By similarity

GO - Cellular componenti

  1. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10881088RNA-directed RNA polymerasePRO_0000368047Add
BLAST

Interactioni

Subunit structurei

Interacts with VP3 (Potential). Interacts with VP2; this interaction activates VP1. Interacts with NSP5; this interaction is probably necessary for the formation of functional virus factories. Interacts with NSP2; this interaction is weak (By similarity).By similarityCurated

Structurei

3D structure databases

ProteinModelPortaliA4ZCW8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini501 – 687187RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR001795. RNA-dir_pol_luteovirus.
IPR007097. RNA-dir_pol_reovirus.
IPR022071. Rotavirus_VP1.
[Graphical view]
PfamiPF02123. RdRP_4. 1 hit.
PF12289. Rotavirus_VP1. 1 hit.
[Graphical view]
PROSITEiPS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4ZCW8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKYNLILSE YLSFVYNSQS AVQIPIYYSS NSELEKRCIE FHAKCVDSSK
60 70 80 90 100
KGLSLKPLFE EYKDVIDNAT LLSILSYSYD KYNAVERKLV NYAKGKPLEA
110 120 130 140 150
DLTANEIDYE NNKITSELFQ SAEEYTDSLM DPAILTSLSS NLNAVMFWLE
160 170 180 190 200
RHSNDVADAN KIYKRRLDLF TIVASTINKY GVPRHNEKYR YEYEVMKDKP
210 220 230 240 250
YYLVTWANSS IEMLMSVFSH EDYLIAKELI ILSYSNRSTL AKLVSSPMSI
260 270 280 290 300
LVALIDINGT FITNEELELE FSDKYVKAIV PDQIFDELQE MIDNMRKAGL
310 320 330 340 350
VDIPRMIQEW LVDCSLEKFT LMSKIYSWSF HVGFRKQKMI DAALDQLKTE
360 370 380 390 400
YTEDVDGEMY NEYTMLIRDE IVKMLEVPVK HDDHLLRDSE LAGLLSMSSA
410 420 430 440 450
SNGESRQLKF GRKTIFSTKK NMHVMDDIAH GRYTPGVIPP VNVDRPIPLG
460 470 480 490 500
RRDVPGRRTR IIFILPYEYF IAQHAVVEKM LSYAKHTREY AEFYSQSNQL
510 520 530 540 550
LSYGDVTRFL SSNSMVLYTD VSQWDSSQHN TQPFRKGIIM GLDMLSNMTN
560 570 580 590 600
DPKVVQTLNL YKQTQINLMD SYVQIPDGNV IKKIQYGAVA SGEKQTKAAN
610 620 630 640 650
SIANLALIKT VLSRIANKYS FITKIIRVDG DDNYAVLQFN TDVTKQMVQD
660 670 680 690 700
VSNDVRYIYS RMNAKVKALV STVGIEIAKR YIAGGKIFFR AGINLLNNEK
710 720 730 740 750
RGQSTQWDQA AILYSNYIVN KLRGFETDRE FILTKIIQMT SVAITGSLRL
760 770 780 790 800
FPSERVLTTN STFKVFDSED FIIEYGTTND EVYIQRAFMS LSSQKSGIAD
810 820 830 840 850
EIASSQTFKN YVNKLSDQLL ISKNVIVSKG IAVTEKAKLN SYAPVYLEKR
860 870 880 890 900
RAQISALLTM LQKPVSFKSN KITINDILRD IKPFFITSEA NLPIQYRKFM
910 920 930 940 950
PTLPNNVQYV IQCIGSRTYQ IEDSGSKSSI SKLISKYSVY KPSIEELYKV
960 970 980 990 1000
ISLREQEIQL YLVSLGVPPV DAGTYVGSRI YSQDKYKILE SYVYNLLSIN
1010 1020 1030 1040 1050
YGCYQLFNFN SPDLEKLIRI PFKGKIPAVT FILHLYAKLE IINYAIKNGA
1060 1070 1080
WISLFCNYPK SEMIKLWKKM WNITALRSPY TSANFFQD
Length:1,088
Mass (Da):124,846
Last modified:May 29, 2007 - v1
Checksum:iD8515EF1F6E9FF39
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371R → G in AAF19585. 1 PublicationCurated
Sequence conflicti105 – 1051N → H in AAD47314. (PubMed:10374958)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ490539 Genomic RNA. Translation: ABF67546.1.
AF044358 Genomic RNA. Translation: AAF19585.1.
AF106300 Genomic RNA. Translation: AAD47314.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ490539 Genomic RNA. Translation: ABF67546.1 .
AF044358 Genomic RNA. Translation: AAF19585.1 .
AF106300 Genomic RNA. Translation: AAD47314.1 .

3D structure databases

ProteinModelPortali A4ZCW8.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001795. RNA-dir_pol_luteovirus.
IPR007097. RNA-dir_pol_reovirus.
IPR022071. Rotavirus_VP1.
[Graphical view ]
Pfami PF02123. RdRP_4. 1 hit.
PF12289. Rotavirus_VP1. 1 hit.
[Graphical view ]
PROSITEi PS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains."
    Matthijnssens J., Ciarlet M., Heiman E.M., Arijs I., Delbeke T., McDonald S.M., Palombo E.A., Iturriza-Gomara M., Maes P., Patton J.T., Rahman M., Van Ranst M.
    J. Virol. 82:3204-3219(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. Lee C.N., Zao C.L.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 8-178.
  3. "Sequence analysis of VP1 and VP7 genes suggests occurrence of a reassortant of G2 rotavirus responsible for an epidemic of gastroenteritis."
    Zao C.L., Yu W.N., Kao C.L., Taniguchi K., Lee C.Y., Lee C.N.
    J. Gen. Virol. 80:1407-1415(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 10-151.

Entry informationi

Entry nameiRDRP_ROTHW
AccessioniPrimary (citable) accession number: A4ZCW8
Secondary accession number(s): Q9QAU9, Q9QS01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 29, 2007
Last modified: November 26, 2014
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3