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A4ZCW5 (VP2_ROTH3) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inner capsid protein VP2
OrganismRotavirus A (strain Human/Japan/AU-1/1982 G3-P3[9]-I3-R3-C3-M3-A3-N3-T3-E3-H3) (RV-A) [Complete proteome]
Taxonomic identifier39013 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length887 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Inner capsid protein that self assembles to form an icosahedral capsid with a T=2 symmetry, which consists of 120 copies of VP2, with channels at each of its five-fold vertices. This capsid constitutes the innermost concentric layer of the viral mature particle. It encapsidates the polymerase VP1, the capping enzyme VP3 and the genomic dsRNA, thereby defining the core. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP2 is required for the replicase activity of VP1 polymerase. It probably plays a role in the coordination of packaging and genome replication by controlling the initiation of minus-strand synthesis. Binding to the polymerase VP1 presumably activates the autoinhibited VP1-RNA complex which will start the synthesis of the complementary minus-strand By similarity.

Subunit structure

Dimer Potential. Interacts with VP1; this interaction presumably activates VP1. Interacts with VP3. Interacts with VP6. Interacts with NSP5 By similarity.

Subcellular location

Virion Potential. Note: Inner capsid protein. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.

Domain

The N-terminus is involved in VP1 binding By similarity.

Sequence similarities

Belongs to the rotavirus VP2 family.

Ontologies

Keywords
   Cellular componentCapsid protein
Inner capsid protein
Virion
   DomainRepeat
   LigandRNA-binding
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentviral inner capsid

Inferred from electronic annotation. Source: UniProtKB-KW

viral nucleocapsid

Inferred from electronic annotation. Source: InterPro

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 887887Inner capsid protein VP2
PRO_0000368056

Regions

Region1 – 9999Interaction with VP1 and VP3 By similarity
Region401 – 42121Hydrophobic By similarity
Region429 – 44921Hydrophobic By similarity
Region543 – 56422Leucine-zipper 1 Potential
Region672 – 69322Leucine-zipper 2 Potential

Sequences

Sequence LengthMass (Da)Tools
A4ZCW5 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: 433AB813153C53B4

FASTA887103,235
        10         20         30         40         50         60 
MAYRKRGARR ETNLKQDDRM QEKEENKSTN SVIENKNGTK TQLSEKVLSQ KEEVITDNQE 

        70         80         90        100        110        120 
ETRIADEVKK SNKEESKQLL EVLKTKEEHQ KEVQYEILQK TIPTFEPKES ILKKLEDIKP 

       130        140        150        160        170        180 
EQAKKQTKLF RIFEPKQLPI YRANGEKELR NRWYWKLKRD TLPDGDYDVR EYFLNLYDQV 

       190        200        210        220        230        240 
LTEMPDYLLL KDMAVENKNS RDAGKVVDSE TAAICDAIFQ DEETEGVVRR FIAEMRQRVQ 

       250        260        270        280        290        300 
ADQNVVNYPS ILHPIDHAFN EYFLQHQLVE PLNNDIIFNY IPERIRNDVN YILNMDRNLP 

       310        320        330        340        350        360 
STARYIRPNL LQDRLNLHDN FESLWDTITT SNYILARSVV PDLKELVSTE AQIQKMSQDL 

       370        380        390        400        410        420 
QLEALTIQSE TQFLTGINSQ AANDCFKTLI AAMLSQRTMS LDFVTTNYMS LISGMWLLTV 

       430        440        450        460        470        480 
IPNDMFIRES LVACQLAIIN TIIYPAFGMQ RMHYRNGDPQ TPFQIAEQQI QNFQVANWLH 

       490        500        510        520        530        540 
FVNNNQFRQV IIDGVLNQVL NDNIRNGHVV NQLMEALMQL SRQQFPTMPV DYKRSIQRGI 

       550        560        570        580        590        600 
LLLSNRLGQL VDLTRLLAYN YETLMACITM NMQHVQTLTT EKLQLTSVTS LCMLIGNATV 

       610        620        630        640        650        660 
IPSPQTLFHY YNVNVNFHSN YNERINDAVA IITAANRLNL YQKKMKSIVE DFLKRLQIFD 

       670        680        690        700        710        720 
VSRVPDDQMY RLRDRLRLLP VEIRRLDIFN LILMNMEQIE RASDKIAQGV IIAYRDMQLE 

       730        740        750        760        770        780 
RDEMYGYVNI ARNLDGFQQI NLEELMRTGD YAQITNMLLN NQPVALVGAL PFITDSSVIS 

       790        800        810        820        830        840 
LIAKLDATVF AQIVKLRKVD TLKPILYKIN SDSNDFYLVA NYDWVPTSTT KVYKQVPQQF 

       850        860        870        880 
DFRASMHMLT SNLTFTVYSD LLAFVSADTV EPINAVAFDN MRIMNEL 

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References

[1]"Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains."
Matthijnssens J., Ciarlet M., Heiman E.M., Arijs I., Delbeke T., McDonald S.M., Palombo E.A., Iturriza-Gomara M., Maes P., Patton J.T., Rahman M., Van Ranst M.
J. Virol. 82:3204-3219(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ490536 Genomic RNA. Translation: ABF67543.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR007779. Rotavirus_VP2.
[Graphical view]
PfamPF05087. Rota_VP2. 1 hit.
[Graphical view]
ProDomPD008866. Rota_VP2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Entry information

Entry nameVP2_ROTH3
AccessionPrimary (citable) accession number: A4ZCW5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 29, 2007
Last modified: October 16, 2013
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families