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A4ZCW2

- RDRP_ROTH3

UniProt

A4ZCW2 - RDRP_ROTH3

Protein

RNA-directed RNA polymerase

Gene
N/A
Organism
Rotavirus A (strain Human/Japan/AU-1/1982 G3-P3[9]-I3-R3-C3-M3-A3-N3-T3-E3-H3) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 29 (01 Oct 2014)
      Sequence version 1 (29 May 2007)
      Previous versions | rss
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    Functioni

    RNA-directed RNA polymerase that is involved in both transcription and genome replication. Together with VP3 capping enzyme, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. One copy of each of the viral (+)RNAs is also recruited during core assembly, together with newly synthesized polymerase complexes and VP2. The polymerase of these novo-formed particles catalyzes the synthesis of complementary minus-strands leading to dsRNA formation. To do so, the polymerase specifically recognizes and binds 4 bases 5'-UGUG-3' in the conserved 3'-sequence of plus-strand RNA templates. VP2 presumably activates the autoinhibited VP1-RNA complex to coordinate packaging and genome replication. Once dsRNA synthesis is complete, the polymerase switches to the transcriptional mode, thus providing secondary transcription By similarity.PROSITE-ProRule annotation

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    Cofactori

    Magnesium.Curated

    GO - Molecular functioni

    1. nucleotide binding Source: UniProtKB-KW
    2. RNA binding Source: UniProtKB-KW
    3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. transcription, DNA-templated Source: InterPro
    2. viral genome replication Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

    Keywords - Biological processi

    Viral RNA replication

    Keywords - Ligandi

    Magnesium, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-directed RNA polymerase (EC:2.7.7.48)
    Alternative name(s):
    Protein VP1
    OrganismiRotavirus A (strain Human/Japan/AU-1/1982 G3-P3[9]-I3-R3-C3-M3-A3-N3-T3-E3-H3) (RV-A)
    Taxonomic identifieri39013 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000001454: Genome

    Subcellular locationi

    Virion Curated
    Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.By similarity

    GO - Cellular componenti

    1. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10881088RNA-directed RNA polymerasePRO_0000368039Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with VP3 Potential. Interacts with VP2; this interaction activates VP1. Interacts with NSP5; this interaction is probably necessary for the formation of functional virus factories. Interacts with NSP2; this interaction is weak By similarity.By similarityCurated

    Structurei

    3D structure databases

    ProteinModelPortaliA4ZCW2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini501 – 687187RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR001795. RNA-dir_pol_luteovirus.
    IPR007097. RNA-dir_pol_reovirus.
    IPR022071. Rotavirus_VP1.
    [Graphical view]
    PfamiPF02123. RdRP_4. 1 hit.
    PF12289. Rotavirus_VP1. 1 hit.
    [Graphical view]
    PROSITEiPS50523. RDRP_DSRNA_REO. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4ZCW2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKYNLILSE YLSFIYNSQS AVQIPIYYSS NSDLEKRCIE FHSKCLENSK     50
    KGLSLKNIFL EYKDVTENAT LLSILSYSYD KYNAVERKLV HYAKGKPLEA 100
    DLNANELDYE NNKLTSDLFP TAEEYTDSLM DPAILTSLSS NLNAVMFWLE 150
    RHANDTAERA KIYKRRLDLF AIVASTVNKY GVPRHNAKYR YEYEVMKDKP 200
    YYLVTWANSS IEMLMSVFSH EDYLIAKELI ILSYSNRSTL AKLVSSPMSI 250
    LVALVDINGT FITNEELELE FSNKYVRAIV PDQTFDELKE ILDNMKKAGL 300
    VDIPKMIQDW LVDCSIEKFP LMAKIYSWSF HVGFRKQKML DAALDQLKTE 350
    YTEDVDDEMY REYTMLIRDE VVKMLEEAVK HDDHLLQDSE LAGLLSMSSA 400
    SNGESRQLKF GRKTIFSTKK NMHVMDDMAN GKYTPGVIPP VNVDKPIPLG 450
    RRDVPGRRTR IIFILPYEYF IAQHAVVEKM LIYAKHTREY AEFYSQSNQL 500
    LSYGDVTRFL SNNAMVLYTD VSQWDSSQHN TQPFRKGIIM GLDILANMTN 550
    DAKVIQTLHL YKQTQINLMD SYVQIPDGNV IKKIQYGAVA SGEKQTKAAN 600
    SIANLALIKT VLSRIANKYS FVTKIIRVDG DDNYAVLQFN TEVTKQMVQD 650
    VSNDVRDTYA RMNAKVKALV STVGIEIAKR YIAGGKIFFR AGINLLNNEK 700
    RGQSTQWDQA AVLYSNYIVN RLRGFETDRE FILTKIMQMT SVAITGSLRL 750
    FPSERVLTTN STFKVFDSED FIIEYGTTDD EVYIQRAFMS LSSQKSGIAD 800
    EIAASQTFKN YVSKLSDQLL VSKNVIVSRG IALTEKAKLN SYAPVSLEKR 850
    RAQISALLTM LQKPVTFKSN KITINDILHD IKPYFVTSEA NLPIQYQKFM 900
    PTLPNNVQYI IQCIGSRTYQ IEDDGSKSAI SRLISKYSVY RPSIEELYKV 950
    ISLHEQEIQL YLVSLGIPKI DADTYVGSKI YSQDKYRILE SYVYNLLSIN 1000
    YGCYQLFDFN SPDLEKLIRI PFKGKIPAVT FILHLYAKLE IINYAIKNGS 1050
    WISLFCNYPK SEMIKLWKKM WNITSLRSPY TNANFFQD 1088
    Length:1,088
    Mass (Da):124,847
    Last modified:May 29, 2007 - v1
    Checksum:iDC2F1D88A0C76706
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ490533 Genomic RNA. Translation: ABF67540.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ490533 Genomic RNA. Translation: ABF67540.1 .

    3D structure databases

    ProteinModelPortali A4ZCW2.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR001795. RNA-dir_pol_luteovirus.
    IPR007097. RNA-dir_pol_reovirus.
    IPR022071. Rotavirus_VP1.
    [Graphical view ]
    Pfami PF02123. RdRP_4. 1 hit.
    PF12289. Rotavirus_VP1. 1 hit.
    [Graphical view ]
    PROSITEi PS50523. RDRP_DSRNA_REO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains."
      Matthijnssens J., Ciarlet M., Heiman E.M., Arijs I., Delbeke T., McDonald S.M., Palombo E.A., Iturriza-Gomara M., Maes P., Patton J.T., Rahman M., Van Ranst M.
      J. Virol. 82:3204-3219(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiRDRP_ROTH3
    AccessioniPrimary (citable) accession number: A4ZCW2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 29 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3