Reviewed, UniProtKB/Swiss-Prot A4Z6H0 (PURA1_PIG)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase isozyme 1
      Short name=AMPSase 1
      Short name=AdSS 1
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase 1

Gene names

Name:	ADSSL1
Synonyms:	ADSS1

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis By similarity.
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylosuccinate synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	Adenylosuccinate synthetase isozyme 1		PRO_0000321961
Regions
	Nucleotide binding	42 – 48	7	GTP Potential
Sites
	Active site	174	1	By similarity
	Active site	181	1	By similarity
	Metal binding	43	1	Magnesium By similarity
	Metal binding	70	1	Magnesium; via carbonyl oxygen By similarity
Amino acid modifications
	Modified residue	388	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4Z6H0-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: CF4DFDAEE379BC66	FASTA	457	50,098
	10 20 30 40 50 60 MSGTRASNDR PPSAGGVKRG RLQHEAATTG SRVTVVLGAQ WGDEGKGKVV DLLATDADII 70 80 90 100 110 120 SRCQGGNNAG HTVVVDGKEY DFHLLPSGII NTKAVSFIGN GVVVHLPGLF EEAEKNEKKG 130 140 150 160 170 180 LKDWEKRLII SDRAHLVFDF HQAVDGLQEV QRQAQEGKNI GTTRKGIGPA YSSKAARAGL 190 200 210 220 230 240 RVCDLLSDFD EFSARFRNLA RQHQSMFPTL ETDVEGQLKK LKGFAERIRP MVRDGVYFMY 250 260 270 280 290 300 EALHGPPKKI LVEGANAALL DIGFGTCPFV TSSNCTVGGV CTGLGIPPQN IGEVYGVVKA 310 320 330 340 350 360 YTTRVGVGAF PTEQINETGD LLQSRGHEWG VTTGRKRRCG WLDLMILRYA HMINGFTALA 370 380 390 400 410 420 LTKLDILDTL DEVKVGVSYK LSGKRIPYFP ANQEILQKVE VEYETLPGWK TDTTGARKWE 430 440 450 DLPPQAQSYI RFVENHVGVA VKWVGVGKSR DSMIQLF

References

[1]	"Comparative molecular characterization of ADSS1 and ADSS2 genes in pig (Sus scrofa)." Li X., Zhu Z., Mo D., Wang H., Yang S., Zhao S., Li K. Comp. Biochem. Physiol. 147B:271-277(2007) [PubMed: 17347008] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ462751 mRNA. Translation: ABE73154.1.
RefSeq	NP_001090978.1.
UniGene	Ssc.24153
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	100048943.
KEGG	ssc:100048943.
Organism-specific databases
CTD	100048943.
Enzyme and pathway databases
BRENDA	6.3.4.4. 249.
Family and domain databases
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
ProDom	PD001188. Asucc_synthtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA1_PIG

Accession

Primary (citable) accession number: A4Z6H0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	May 29, 2007
Last modified:	September 1, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)