Reviewed, UniProtKB/Swiss-Prot A4Z2G7 (PYRD_BRASO)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.5.2
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	BRADO6742

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + a quinone = orotate + a quinol. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. HAMAP MF_00225
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_1000024156
Sites
	Active site	173	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4Z2G7-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: AA9B41BB22A0DF17	FASTA	364	39,208
	10 20 30 40 50 60 MIRAFDQLSL PLLRWLDAED AHRLAIQGLK LLPAVKPRAD DAKLAVRAFG LNFPNPVGMA 70 80 90 100 110 120 AGFDKNAEVP DALLRLGFGF VEIGSVTPRP QSGNPRPRLF RLERDEAVIN RMGFNNDGAE 130 140 150 160 170 180 IVLRRLAGRA NQGGIVGVNV GANKDSADRV ADYVRLIETF APVASYFTVN ISSPNTPGLR 190 200 210 220 230 240 NLQQASQLDD LLTKVLEARD RVRRKAGDTP VLLKIAPDLS LAELDDVVHV ARSRGVDGMI 250 260 270 280 290 300 VSNTTLARPN SLREQMRAKE QGGLSGRPLF RLSTRMVAET FVRVEGAFPL IGVGGIDTGG 310 320 330 340 350 360 AALTKIRAGA SLIQLYSSLV YKGLGLVESI KADLTSTLLR TGRESLSEIV GADAATITAE DWPV

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU234118 Genomic DNA. Translation: CAL80343.1.
RefSeq	YP_001208558.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4Z2G7.
Genome annotation databases
GeneID	5119338.
GenomeReviews	Gene locus BRADO6742 in contig CU234118_GR.
KEGG	bra:BRADO6742.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	AALNRMG.
Family and domain databases
HAMAP	MF_00225. [Tree]
InterPro	IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_BRASO

Accession

Primary (citable) accession number: A4Z2G7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)