Reviewed, UniProtKB/Swiss-Prot A4Z1S1 (ARGC_BRASO)

Names and origin

Protein names

Recommended name:
    N-acetyl-gamma-glutamyl-phosphate reductase
      Short name=AGPR
    EC=1.2.1.38
Alternative name(s):
    N-acetyl-glutamate semialdehyde dehydrogenase
      Short name=NAGSA dehydrogenase

Gene names

Name:	argC
Ordered Locus Names:	BRADO6484

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate = N-acetyl-5-glutamyl phosphate + NADPH. HAMAP MF_00150
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 3/4. HAMAP MF_00150
Subcellular location	Cytoplasm Probable HAMAP MF_00150.
Sequence similarities	Belongs to the NAGSA dehydrogenase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetyl-gamma-glutamyl-phosphate reductase activity Inferred from electronic annotation. Source: HAMAP NAD or NADH binding Inferred from electronic annotation. Source: InterPro protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	N-acetyl-gamma-glutamyl-phosphate reductase HAMAP MF_00150		PRO_1000010982
Sites
	Active site	131	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4Z1S1-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 429595113E528006	FASTA	324	33,704
	10 20 30 40 50 60 MSIRVGIVGI SGFGGGEAMR LIAGHPAFEP VYAAGEGSAG QRLSERFPGV PAKLADLVIE 70 80 90 100 110 120 KWDPSRLPQL DVLFASLPTG ASRDALARIP REVKIVDIGG DHRYADGWTY GLADVWPEAI 130 140 150 160 170 180 AGKTRIANPG CFPAAALTAL APLLADRLIA PENIVIDAKT GISGAGRGGG AGFGYAESNE 190 200 210 220 230 240 NLIPYGLLKH VHMPEIESTI ARISGGSAAG LVFTPHLVPM TRGILATIYA RGRATSDQCL 250 260 270 280 290 300 DAARSFYAGR AFVRVTDKPP QTKWATGSNL AFVSYAADPD RNLVIALGVV DNLGKGAAGQ 310 320 AVQNANLMCG LPETAGLEGA PVWP

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU234118 Genomic DNA. Translation: CAL80097.1.
RefSeq	YP_001208312.1.
3D structure databases
SMR	A4Z1S1. Positions 3-324.
ModBase	Search...
Protein-protein interaction databases
STRING	A4Z1S1.
Genome annotation databases
GeneID	5115771.
GenomeReviews	Gene locus BRADO6484 in contig CU234118_GR.
KEGG	bra:BRADO6484.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0002.
HOGENOM	HBG294213.
OMA	FGGGEAM.
Family and domain databases
HAMAP	MF_00150. ArgC_type1. [Tree]
InterPro	IPR000706. AGPR_act_site. IPR016040. NAD(P)-bd_dom. IPR000534. Semialdehyde_DH_NAD-bd. IPR012280. Semialdhyde_DH_dimer_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01118. Semialdhyde_dh. 1 hit. PF02774. Semialdhyde_dhC. 1 hit. [Graphical view]
SMART	SM00859. Semialdhyde_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01850. argC. 1 hit.
PROSITE	PS01224. ARGC. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGC_BRASO

Accession

Primary (citable) accession number: A4Z1S1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 29, 2007
Last modified:	February 9, 2010
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)