A4Z165 (A4Z165_BRASO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Ferrochelatase HAMAP MF_00323 RuleBase RU000607
EC=4.99.1.1 HAMAP MF_00323 RuleBase RU000607

Alternative name(s):
Heme synthase HAMAP MF_00323
Protoheme ferro-lyase HAMAP MF_00323

Gene names

Name:	hemH HAMAP MF_00323 EMBL CAL79891.1
Ordered Locus Names:	BRADO6249

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX By similarity. HAMAP MF_00323 RuleBase RU000607 SAAS SAAS001015
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323 RuleBase RU000607 SAAS SAAS001015
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323 RuleBase RU000607 SAAS SAAS001015
Subcellular location	Cytoplasm By similarity HAMAP MF_00323.
Sequence similarities	Belongs to the ferrochelatase family. HAMAP MF_00323 RuleBase RU004185

Ontologies

Keywords
Biological process	Heme biosynthesis HAMAP MF_00323 RuleBase RU000607 SAAS SAAS001015 Porphyrin biosynthesis HAMAP MF_00323 RuleBase RU000607 SAAS SAAS001015
Cellular component	Cytoplasm HAMAP MF_00323
Ligand	Iron HAMAP MF_00323 RuleBase RU000607 SAAS SAAS001015 Metal-binding HAMAP MF_00323 SAAS SAAS001015
Molecular function	Lyase HAMAP MF_00323 RuleBase RU000607 SAAS SAAS001015 EMBL CAL79891.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	235	1	Iron By similarity HAMAP MF_00323
	Metal binding	316	1	Iron By similarity HAMAP MF_00323

Sequences

	Sequence		Length	Mass (Da)
	A4Z165 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: D14C5D2F942AD968	FASTA	365	40,834
	10 20 30 40 50 60 MTAAISLDNA RSVEAKPVEA KPVEAKPVDL KPSSAAKTER VGVLLVNLGT PDTADAAGVR 70 80 90 100 110 120 VYLKEFLSDR RVIEDQGLLW KIILNGIILN VRPRKKAKDY QSIWNNEKNE SPLKTITRAQ 130 140 150 160 170 180 SEKLAAAMAD RSHVIVDWAM RYGNPSMKAG IDGLKAKGCD RILVVPLYPQ YSAATSATVC 190 200 210 220 230 240 DEAFRVLTEM RAQPTIRVTP PYYNDDFYIE ALAVSIEEHL KTLSYTPELI VASFHGMPKE 250 260 270 280 290 300 YVDKGDPYRE QCIATTELLR KRLGMDETKL LLTFQSRFGF SEWLQPYTDK TIEKLAKDGV 310 320 330 340 350 360 KRMAVVMPGF SADCLETLEE ISGENCEIFK HNGGEEFSAV PCLNDSEPGM EVLRQLVLRE LQGWL

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU234118 Genomic DNA. Translation: CAL79891.1.
RefSeq	YP_001208106.1. NC_009445.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4Z165.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5119074.
GenomeReviews	Gene locus BRADO6249 in contig CU234118_GR.
KEGG	bra:BRADO6249.
PATRIC	21228407. VBIBraSp122330_5997.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0276.
HOGENOM	HBG697135.
OMA	TIEEIGM.
ProtClustDB	PRK00035.
Family and domain databases
HAMAP	MF_00323. Ferrochelatase. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
KO	K01772.
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. HemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A4Z165_BRASO

Accession

Primary (citable) accession number: A4Z165

Entry history

Integrated into UniProtKB/TrEMBL:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)