A4Z0S1 (LPXK_BRASO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraacyldisaccharide 4'-kinase
EC=2.7.1.130

Alternative name(s):
Lipid A 4'-kinase

Gene names

Name:	lpxK
Ordered Locus Names:	BRADO6095

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity. HAMAP MF_00409
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP MF_00409
Sequence similarities	Belongs to the LpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tetraacyldisaccharide 4'-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Tetraacyldisaccharide 4'-kinase HAMAP MF_00409		PRO_1000080460
Regions
	Nucleotide binding	51 – 58	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A4Z0S1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 4BEC3551081ADD0E	FASTA	335	35,392
	10 20 30 40 50 60 MREPAFWHRP PSWQSHLLSP LSMLYGAVAA RRMAQPGIKA GVPVICVGNY HVGGAGKTPT 70 80 90 100 110 120 VLALTALLRG QGEQPVVLSR GYGGRLPGPV LVDPAAHGAA DVGDEPLMMA AHVPVVVSRA 130 140 150 160 170 180 RADGVGLAKA HRASVILMDD GFQNPSITKD LALIVVDGGR GLGNARVFPA GPLRTPLPPQ 190 200 210 220 230 240 LARTDALMII GRGEAGEAVA SRVAAAGKPV FRAQLQPDAG VVASLAGRPL LAFAGIGDPQ 250 260 270 280 290 300 RFFRSLRASG LEIRAERPFP DHHPFTDGDI KALVDQATRE QLALVTTEKD LVRLRGRGWD 310 320 330 HPELAPMAFP VTLQFDDETA VRSLVARRLS AARAS

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ORS278.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU234118 Genomic DNA. Translation: CAL79747.1.
RefSeq	YP_001207962.1. NC_009445.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4Z0S1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5117205.
GenomeReviews	Gene locus BRADO6095 in contig CU234118_GR.
KEGG	bra:BRADO6095.
PATRIC	21228127. VBIBraSp122330_5858.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1663.
HOGENOM	HBG663914.
OMA	VILMDDG.
ProtClustDB	PRK00652.
Enzyme and pathway databases
BioCyc	BSP376:BRADO6095-MONOMER.
Family and domain databases
HAMAP	MF_00409. LpxK. [Tree]
InterPro	IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
KO	K00912.
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. LpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_BRASO

Accession

Primary (citable) accession number: A4Z0S1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 29, 2007
Last modified:	December 14, 2011
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program