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A4Z0A1

- NAPA_BRASO

UniProt

A4Z0A1 - NAPA_BRASO

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Bradyrhizobium sp. (strain ORS278)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (29 May 2007)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi54 – 541Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi58 – 581Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi86 – 861Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciBSP114615:GJN5-5656-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:BRADO5916
    OrganismiBradyrhizobium sp. (strain ORS278)
    Taxonomic identifieri114615 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
    ProteomesiUP000001994: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232Tat-type signalUniRule annotationAdd
    BLAST
    Chaini33 – 834802Periplasmic nitrate reductasePRO_1000069710Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi114615.BRADO5916.

    Structurei

    3D structure databases

    ProteinModelPortaliA4Z0A1.
    SMRiA4Z0A1. Positions 44-832.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini44 – 100574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiHPKTRVA.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A4Z0A1-1 [UniParc]FASTAAdd to Basket

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    MTEPKIDRRQ LLKLEAAAIA AAAAGMPTVA RAANLVTERE ATELKWDKAA    50
    CRFCGTGCSV MVATKDNRVV ATHGDIKAEV NRGLNCVKGY FLSKIMYGHD 100
    RLTHPMLRKT GGQYDKNGEF TPVSWDEAFD VMALKFKDAL KKRGPSGVGM 150
    FGSGQWTIWE GYAASKLFKA GFRTNNIDPN ARHCMASAVA GMMRTFGIDE 200
    PAGCYDDIEA TDAFVLWGSN MAEMHPILWT RVTDRRLSAP HVKVAVLSTF 250
    EHRSFDLADV GMVFKPQTDL YILNAIANHI ISTGRVNKDF VSAHTVFKKG 300
    QTDIGYGLRP EHPLQKKATG AAKANDATDI SFDEYAKFVS DYTLEKAADM 350
    SGVPLNRLQA LAELYADPKT KVVSFWTMGF NQHTRGVWCN NLVYNIHLLT 400
    GKIAEAGNSP FSLTGQPSAC GTAREVGTFS HRLPADMVVT NKEHRTKAEH 450
    IWQLPEGTIP DKPGYHAVLQ SRMLKDGLLN AYWVQVNNNL QAGPNANEET 500
    YPGFRNPDNF IVVSDAYPSV TALAADLILP TAMWVEKEGA YGNAERRTQF 550
    WHQLVSAPGE ARSDLWQLME FSKRFKIEDV WPEELIAKKP DVRGKTLFDV 600
    LYKNGQVDKF PLDQIEPGYA NDESKAFGFY VHKGLFEEYA SFGRGHGHDL 650
    APFEAYHKER GLRWPVVDGK ETRWRFREGS DPYVKAGTGV QFYGFPDGKA 700
    RIFALPYEPP AESPDKDYPF WLSTGRVVEH WHSGTMTRRV PELYKAFPEA 750
    VCFMHPDDAQ EANLRRGDEV KVASRRGFIR ARVETRGRDK PPRGLVFVPW 800
    FDESKLINKV TLDATDPISL QTDYKKCAVR IERV 834
    Length:834
    Mass (Da):93,492
    Last modified:May 29, 2007 - v1
    Checksum:iABD57C8E3E5A55EF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU234118 Genomic DNA. Translation: CAL79577.1.
    RefSeqiWP_012029476.1. NC_009445.1.
    YP_001207792.1. NC_009445.1.

    Genome annotation databases

    EnsemblBacteriaiCAL79577; CAL79577; BRADO5916.
    GeneIDi5120295.
    KEGGibra:BRADO5916.
    PATRICi21227775. VBIBraSp122330_5682.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU234118 Genomic DNA. Translation: CAL79577.1 .
    RefSeqi WP_012029476.1. NC_009445.1.
    YP_001207792.1. NC_009445.1.

    3D structure databases

    ProteinModelPortali A4Z0A1.
    SMRi A4Z0A1. Positions 44-832.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 114615.BRADO5916.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAL79577 ; CAL79577 ; BRADO5916 .
    GeneIDi 5120295.
    KEGGi bra:BRADO5916.
    PATRICi 21227775. VBIBraSp122330_5682.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi HPKTRVA.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci BSP114615:GJN5-5656-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ORS278.

    Entry informationi

    Entry nameiNAPA_BRASO
    AccessioniPrimary (citable) accession number: A4Z0A1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3