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A4Z0A1 (NAPA_BRASO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:BRADO5916
OrganismBradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]
Taxonomic identifier114615 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length834 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232Tat-type signal Potential
Chain33 – 834802Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_1000069710

Regions

Domain44 – 100574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding541Iron-sulfur (4Fe-4S) By similarity
Metal binding581Iron-sulfur (4Fe-4S) By similarity
Metal binding861Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A4Z0A1 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: ABD57C8E3E5A55EF

FASTA83493,492
        10         20         30         40         50         60 
MTEPKIDRRQ LLKLEAAAIA AAAAGMPTVA RAANLVTERE ATELKWDKAA CRFCGTGCSV 

        70         80         90        100        110        120 
MVATKDNRVV ATHGDIKAEV NRGLNCVKGY FLSKIMYGHD RLTHPMLRKT GGQYDKNGEF 

       130        140        150        160        170        180 
TPVSWDEAFD VMALKFKDAL KKRGPSGVGM FGSGQWTIWE GYAASKLFKA GFRTNNIDPN 

       190        200        210        220        230        240 
ARHCMASAVA GMMRTFGIDE PAGCYDDIEA TDAFVLWGSN MAEMHPILWT RVTDRRLSAP 

       250        260        270        280        290        300 
HVKVAVLSTF EHRSFDLADV GMVFKPQTDL YILNAIANHI ISTGRVNKDF VSAHTVFKKG 

       310        320        330        340        350        360 
QTDIGYGLRP EHPLQKKATG AAKANDATDI SFDEYAKFVS DYTLEKAADM SGVPLNRLQA 

       370        380        390        400        410        420 
LAELYADPKT KVVSFWTMGF NQHTRGVWCN NLVYNIHLLT GKIAEAGNSP FSLTGQPSAC 

       430        440        450        460        470        480 
GTAREVGTFS HRLPADMVVT NKEHRTKAEH IWQLPEGTIP DKPGYHAVLQ SRMLKDGLLN 

       490        500        510        520        530        540 
AYWVQVNNNL QAGPNANEET YPGFRNPDNF IVVSDAYPSV TALAADLILP TAMWVEKEGA 

       550        560        570        580        590        600 
YGNAERRTQF WHQLVSAPGE ARSDLWQLME FSKRFKIEDV WPEELIAKKP DVRGKTLFDV 

       610        620        630        640        650        660 
LYKNGQVDKF PLDQIEPGYA NDESKAFGFY VHKGLFEEYA SFGRGHGHDL APFEAYHKER 

       670        680        690        700        710        720 
GLRWPVVDGK ETRWRFREGS DPYVKAGTGV QFYGFPDGKA RIFALPYEPP AESPDKDYPF 

       730        740        750        760        770        780 
WLSTGRVVEH WHSGTMTRRV PELYKAFPEA VCFMHPDDAQ EANLRRGDEV KVASRRGFIR 

       790        800        810        820        830 
ARVETRGRDK PPRGLVFVPW FDESKLINKV TLDATDPISL QTDYKKCAVR IERV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU234118 Genomic DNA. Translation: CAL79577.1.
RefSeqYP_001207792.1. NC_009445.1.

3D structure databases

ProteinModelPortalA4Z0A1.
SMRA4Z0A1. Positions 44-832.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING114615.BRADO5916.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL79577; CAL79577; BRADO5916.
GeneID5120295.
KEGGbra:BRADO5916.
PATRIC21227775. VBIBraSp122330_5682.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAHPKTRVA.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycBSP114615:GJN5-5656-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_BRASO
AccessionPrimary (citable) accession number: A4Z0A1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 29, 2007
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families