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A4Z0A1

- NAPA_BRASO

UniProt

A4Z0A1 - NAPA_BRASO

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Protein
Periplasmic nitrate reductase
Gene
napA, BRADO5916
Organism
Bradyrhizobium sp. (strain ORS278)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi51 – 511Iron-sulfur (4Fe-4S) By similarity
Metal bindingi54 – 541Iron-sulfur (4Fe-4S) By similarity
Metal bindingi58 – 581Iron-sulfur (4Fe-4S) By similarity
Metal bindingi86 – 861Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciBSP114615:GJN5-5656-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:BRADO5916
OrganismiBradyrhizobium sp. (strain ORS278)
Taxonomic identifieri114615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000001994: Chromosome

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Tat-type signal Reviewed prediction
Add
BLAST
Chaini33 – 834802Periplasmic nitrate reductaseUniRule annotation
PRO_1000069710Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi114615.BRADO5916.

Structurei

3D structure databases

ProteinModelPortaliA4Z0A1.
SMRiA4Z0A1. Positions 44-832.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 100574Fe-4S Mo/W bis-MGD-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiHPKTRVA.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A4Z0A1-1 [UniParc]FASTAAdd to Basket

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MTEPKIDRRQ LLKLEAAAIA AAAAGMPTVA RAANLVTERE ATELKWDKAA    50
CRFCGTGCSV MVATKDNRVV ATHGDIKAEV NRGLNCVKGY FLSKIMYGHD 100
RLTHPMLRKT GGQYDKNGEF TPVSWDEAFD VMALKFKDAL KKRGPSGVGM 150
FGSGQWTIWE GYAASKLFKA GFRTNNIDPN ARHCMASAVA GMMRTFGIDE 200
PAGCYDDIEA TDAFVLWGSN MAEMHPILWT RVTDRRLSAP HVKVAVLSTF 250
EHRSFDLADV GMVFKPQTDL YILNAIANHI ISTGRVNKDF VSAHTVFKKG 300
QTDIGYGLRP EHPLQKKATG AAKANDATDI SFDEYAKFVS DYTLEKAADM 350
SGVPLNRLQA LAELYADPKT KVVSFWTMGF NQHTRGVWCN NLVYNIHLLT 400
GKIAEAGNSP FSLTGQPSAC GTAREVGTFS HRLPADMVVT NKEHRTKAEH 450
IWQLPEGTIP DKPGYHAVLQ SRMLKDGLLN AYWVQVNNNL QAGPNANEET 500
YPGFRNPDNF IVVSDAYPSV TALAADLILP TAMWVEKEGA YGNAERRTQF 550
WHQLVSAPGE ARSDLWQLME FSKRFKIEDV WPEELIAKKP DVRGKTLFDV 600
LYKNGQVDKF PLDQIEPGYA NDESKAFGFY VHKGLFEEYA SFGRGHGHDL 650
APFEAYHKER GLRWPVVDGK ETRWRFREGS DPYVKAGTGV QFYGFPDGKA 700
RIFALPYEPP AESPDKDYPF WLSTGRVVEH WHSGTMTRRV PELYKAFPEA 750
VCFMHPDDAQ EANLRRGDEV KVASRRGFIR ARVETRGRDK PPRGLVFVPW 800
FDESKLINKV TLDATDPISL QTDYKKCAVR IERV 834
Length:834
Mass (Da):93,492
Last modified:May 29, 2007 - v1
Checksum:iABD57C8E3E5A55EF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU234118 Genomic DNA. Translation: CAL79577.1.
RefSeqiWP_012029476.1. NC_009445.1.
YP_001207792.1. NC_009445.1.

Genome annotation databases

EnsemblBacteriaiCAL79577; CAL79577; BRADO5916.
GeneIDi5120295.
KEGGibra:BRADO5916.
PATRICi21227775. VBIBraSp122330_5682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU234118 Genomic DNA. Translation: CAL79577.1 .
RefSeqi WP_012029476.1. NC_009445.1.
YP_001207792.1. NC_009445.1.

3D structure databases

ProteinModelPortali A4Z0A1.
SMRi A4Z0A1. Positions 44-832.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 114615.BRADO5916.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL79577 ; CAL79577 ; BRADO5916 .
GeneIDi 5120295.
KEGGi bra:BRADO5916.
PATRICi 21227775. VBIBraSp122330_5682.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi HPKTRVA.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci BSP114615:GJN5-5656-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ORS278.

Entry informationi

Entry nameiNAPA_BRASO
AccessioniPrimary (citable) accession number: A4Z0A1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 29, 2007
Last modified: September 3, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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