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A4Z005

- GLGB_BRASO

UniProt

A4Z005 - GLGB_BRASO

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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Bradyrhizobium sp. (strain ORS278)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei397 – 3971NucleophileUniRule annotation
Active sitei450 – 4501Proton donorUniRule annotation

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciBSP114615:GJN5-5558-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:BRADO5817
OrganismiBradyrhizobium sp. (strain ORS278)
Taxonomic identifieri114615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000001994: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7127121,4-alpha-glucan branching enzyme GlgBPRO_1000061988Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi114615.BRADO5817.

Structurei

3D structure databases

ProteinModelPortaliA4Z005.
SMRiA4Z005. Positions 110-712.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiAKLLFMG.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

A4Z005-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTHLSPEAFA IIEGRHADPF RYLGQHNVDG QTIVRAFLPE ASRVEVVGEH
60 70 80 90 100
GEVAPLARIH DAGLFTGTMS SMQRYRLRAT FGDSVTDLED PYRFLPILGD
110 120 130 140 150
FDLHLLGEGN HERLYDKLGA HPMVIDGVDG VGFVVLAPNA RRVSVVGDFN
160 170 180 190 200
FWNARRHPMR VRGNGYWELF IPRARAGDHY KFDIIGPQGE HLPLKSDPMA
210 220 230 240 250
FAAEMRPKTA SIVVDETRLP RPRPAPHDIN KLNKPVSIYE VHLGSWRRKD
260 270 280 290 300
NNQWLTYREL AEQLPAYARD MGFTHIEFLP INEHPFDGSW GYQPTGLYAP
310 320 330 340 350
TSRFGSPEDF CALVDACHRE GLAVWLDWVP GHFPDDPHGL GHFDGTALYE
360 370 380 390 400
HANPMQGRHL DWGTLIYNYG RTEVANFLRS NALFWLERYG IDGLRVDAVA
410 420 430 440 450
SMLYLDYSRP SGGWIPNKYG GRENLEAIEF LRRTNIDVFG HFPQATTAAE
460 470 480 490 500
ESTAWPQVSR PVDTGGLGFG YKWNMGWMHD TLRYVSKDPI HRKYHHGEIL
510 520 530 540 550
FGLHYAFSEN FILPLSHDEV VHGKRSILGR MPGDDWQRFA NLRAYYSFMF
560 570 580 590 600
GHPGKKLLFM GCELAQEREW NHDTSLDWHL LGDARYAGIQ ALIRDLNHLY
610 620 630 640 650
RNQPALHERD CDPEGFEWLI TDDADRNVFA WVRKGFDERA HCVVVVNFSP
660 670 680 690 700
NVYYNYRVRA PLGGTWREVF NSDSSHYGGS NVGNVGQVHA SEDQHLNLIL
710
PPMAAVFLVP EA
Length:712
Mass (Da):81,178
Last modified:May 29, 2007 - v1
Checksum:i2C98C9B7DC94AAA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU234118 Genomic DNA. Translation: CAL79481.1.
RefSeqiWP_012029384.1. NC_009445.1.
YP_001207698.1. NC_009445.1.

Genome annotation databases

EnsemblBacteriaiCAL79481; CAL79481; BRADO5817.
GeneIDi5115554.
KEGGibra:BRADO5817.
PATRICi21227577. VBIBraSp122330_5585.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU234118 Genomic DNA. Translation: CAL79481.1 .
RefSeqi WP_012029384.1. NC_009445.1.
YP_001207698.1. NC_009445.1.

3D structure databases

ProteinModelPortali A4Z005.
SMRi A4Z005. Positions 110-712.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 114615.BRADO5817.

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL79481 ; CAL79481 ; BRADO5817 .
GeneIDi 5115554.
KEGGi bra:BRADO5817.
PATRICi 21227577. VBIBraSp122330_5585.

Phylogenomic databases

eggNOGi COG0296.
HOGENOMi HOG000283037.
KOi K00700.
OMAi AKLLFMG.
OrthoDBi EOG6JX7GT.

Enzyme and pathway databases

UniPathwayi UPA00164 .
BioCyci BSP114615:GJN5-5558-MONOMER.

Family and domain databases

Gene3Di 2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_00685. GlgB.
InterProi IPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
PIRSFi PIRSF000463. GlgB. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ORS278.

Entry informationi

Entry nameiGLGB_BRASO
AccessioniPrimary (citable) accession number: A4Z005
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 29, 2007
Last modified: October 1, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3