Reviewed, UniProtKB/Swiss-Prot A4Z005 (GLGB_BRASO)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	BRADO5817

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	712 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 712	712	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_1000061988
Sites
	Active site	292	1	By similarity
	Active site	327	1	By similarity
	Active site	332	1	By similarity
	Active site	395	1	By similarity
	Active site	397	1	By similarity
	Active site	450	1	By similarity
	Active site	517	1	By similarity
	Active site	518	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4Z005-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 2C98C9B7DC94AAA2	FASTA	712	81,178
	10 20 30 40 50 60 MTHLSPEAFA IIEGRHADPF RYLGQHNVDG QTIVRAFLPE ASRVEVVGEH GEVAPLARIH 70 80 90 100 110 120 DAGLFTGTMS SMQRYRLRAT FGDSVTDLED PYRFLPILGD FDLHLLGEGN HERLYDKLGA 130 140 150 160 170 180 HPMVIDGVDG VGFVVLAPNA RRVSVVGDFN FWNARRHPMR VRGNGYWELF IPRARAGDHY 190 200 210 220 230 240 KFDIIGPQGE HLPLKSDPMA FAAEMRPKTA SIVVDETRLP RPRPAPHDIN KLNKPVSIYE 250 260 270 280 290 300 VHLGSWRRKD NNQWLTYREL AEQLPAYARD MGFTHIEFLP INEHPFDGSW GYQPTGLYAP 310 320 330 340 350 360 TSRFGSPEDF CALVDACHRE GLAVWLDWVP GHFPDDPHGL GHFDGTALYE HANPMQGRHL 370 380 390 400 410 420 DWGTLIYNYG RTEVANFLRS NALFWLERYG IDGLRVDAVA SMLYLDYSRP SGGWIPNKYG 430 440 450 460 470 480 GRENLEAIEF LRRTNIDVFG HFPQATTAAE ESTAWPQVSR PVDTGGLGFG YKWNMGWMHD 490 500 510 520 530 540 TLRYVSKDPI HRKYHHGEIL FGLHYAFSEN FILPLSHDEV VHGKRSILGR MPGDDWQRFA 550 560 570 580 590 600 NLRAYYSFMF GHPGKKLLFM GCELAQEREW NHDTSLDWHL LGDARYAGIQ ALIRDLNHLY 610 620 630 640 650 660 RNQPALHERD CDPEGFEWLI TDDADRNVFA WVRKGFDERA HCVVVVNFSP NVYYNYRVRA 670 680 690 700 710 PLGGTWREVF NSDSSHYGGS NVGNVGQVHA SEDQHLNLIL PPMAAVFLVP EA

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU234118 Genomic DNA. Translation: CAL79481.1.
RefSeq	YP_001207698.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4Z005.
Genome annotation databases
GeneID	5115554.
GenomeReviews	Gene locus BRADO5817 in contig CU234118_GR.
KEGG	bra:BRADO5817.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	PKTASIV.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 2 hits. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_BRASO

Accession

Primary (citable) accession number: A4Z005

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)