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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Bradyrhizobium sp. (strain ORS 278)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei196SubstrateUniRule annotation1
Active sitei197Proton donor/acceptorUniRule annotation1
Active sitei327UniRule annotation1
Binding sitei328SubstrateUniRule annotation1
Sitei340Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotationImported
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotationImported
Ordered Locus Names:BRADO5584Imported
OrganismiBradyrhizobium sp. (strain ORS 278)Imported
Taxonomic identifieri114615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000001994 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi114615.BRADO5584.

Structurei

3D structure databases

ProteinModelPortaliA4YZD0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 351Lyase_1InterPro annotationAdd BLAST331
Domaini417 – 470FumaraseC_CInterPro annotationAdd BLAST54

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni107 – 109Substrate bindingUniRule annotation3
Regioni138 – 141Substrate binding (B site)UniRule annotation4
Regioni148 – 150Substrate bindingUniRule annotation3
Regioni333 – 335Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q. Bacteria.
COG0114. LUCA.
HOGENOMiHOG000061736.
KOiK01679.
OMAiVSFTDNC.
OrthoDBiPOG091H01XG.

Family and domain databases

CDDicd01362. Fumarase_classII. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

A4YZD0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATSASSSSS GPTRTETDSF GPIEVAADRY WGAQTERSRQ NFRIGHDRMP
60 70 80 90 100
LGVVHALALV KLAAAKTNRE LGLLDPAMAD AIVQAAQEII DGKLDDHFPL
110 120 130 140 150
VVWQTGSGTQ TNMNLNEVIA NRASELMGGE RGTKKPVHPN DHVNMSQSSN
160 170 180 190 200
DSFPTAMHIA AARAISGHLV PALSELLAAL RAKQDAFADI VKIGRTHTQD
210 220 230 240 250
ATPLTLGQEF SGYAAQVESA ITRIKTAAHD LLPLAQGGTA VGTGLNAHPD
260 270 280 290 300
FAGLFARHVA DITGLAFTTA ANKFEALASN DAYVFAHGAI NAAATGLFKI
310 320 330 340 350
ANDIRFLGSG PRSGLGELIL PENEPGSSIM PGKVNPTQCE ALTMVCCQVF
360 370 380 390 400
GNHTAITMAG SQGHFELNVY KPLLAHCMMQ SIQLLGDAAR SFHEHCVTGI
410 420 430 440 450
QADETRIREL MQRSLMLVTA LAPKIGYDNA AKVAKTAHQR GTTLKEEAVR
460 470
LGLVSEDEFD RLVQPEKMTR PGRS
Length:474
Mass (Da):50,555
Last modified:May 29, 2007 - v1
Checksum:i6E393789DDD8271C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU234118 Genomic DNA. Translation: CAL79256.1.
RefSeqiWP_012029166.1. NC_009445.1.

Genome annotation databases

EnsemblBacteriaiCAL79256; CAL79256; BRADO5584.
KEGGibra:BRADO5584.

Similar proteinsi

Entry informationi

Entry nameiA4YZD0_BRASO
AccessioniPrimary (citable) accession number: A4YZD0
Entry historyiIntegrated into UniProtKB/TrEMBL: May 29, 2007
Last sequence update: May 29, 2007
Last modified: September 27, 2017
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported