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Protein

Bifunctional protein HldE

Gene

hldE

Organism
Bradyrhizobium sp. (strain ORS 278)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7-phosphate at the C-1 position to selectively form D-glycero-beta-D-manno-heptose-1,7-bisphosphate.UniRule annotation
Catalyzes the ADP transfer from ATP to D-glycero-beta-D-manno-heptose 1-phosphate, yielding ADP-D-glycero-beta-D-manno-heptose.UniRule annotation

Catalytic activityi

D-glycero-beta-D-manno-heptose 7-phosphate + ATP = D-glycero-beta-D-manno-heptose 1,7-bisphosphate + ADP.UniRule annotation
D-glycero-beta-D-manno-heptose 1-phosphate + ATP = ADP-D-glycero-beta-D-manno-heptose + diphosphate.UniRule annotation

Pathwayi: ADP-L-glycero-beta-D-manno-heptose biosynthesis

This protein is involved in step 1 and 3 of the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Bifunctional protein HldE (hldE), Bifunctional protein HldE (BRADO5164)
  2. no protein annotated in this organism
  3. Bifunctional protein HldE (hldE), Bifunctional protein HldE (BRADO5164)
  4. ADP-L-glycero-D-manno-heptose-6-epimerase (rfaD)
This subpathway is part of the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate, the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei275UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi205 – 208ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Multifunctional enzyme, Nucleotidyltransferase, Transferase
Biological processCarbohydrate metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSP114615:G1GI7-4940-MONOMER
UniPathwayiUPA00356; UER00437
UPA00356; UER00439

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein HldEUniRule annotation
Including the following 2 domains:
D-beta-D-heptose 7-phosphate kinaseUniRule annotation (EC:2.7.1.167UniRule annotation)
Alternative name(s):
D-beta-D-heptose 7-phosphotransferaseUniRule annotation
D-glycero-beta-D-manno-heptose-7-phosphate kinaseUniRule annotation
D-beta-D-heptose 1-phosphate adenylyltransferaseUniRule annotation (EC:2.7.7.70UniRule annotation)
Alternative name(s):
D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferaseUniRule annotation
Gene namesi
Name:hldEUniRule annotation
Ordered Locus Names:BRADO5201
OrganismiBradyrhizobium sp. (strain ORS 278)
Taxonomic identifieri114615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000001994 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000693981 – 490Bifunctional protein HldEAdd BLAST490

Proteomic databases

PRIDEiA4YYB6

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi114615.BRADO5201

Structurei

3D structure databases

ProteinModelPortaliA4YYB6
SMRiA4YYB6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 330RibokinaseAdd BLAST330
Regioni358 – 490CytidylyltransferaseAdd BLAST133

Sequence similaritiesi

In the N-terminal section; belongs to the carbohydrate kinase PfkB family.UniRule annotation
In the C-terminal section; belongs to the cytidylyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DW0 Bacteria
COG2870 LUCA
HOGENOMiHOG000237584
KOiK03272
OMAiCDPKGKD
OrthoDBiPOG091H01Y4

Family and domain databases

CDDicd01172 RfaE_like, 1 hit
Gene3Di3.40.1190.20, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_01603 HldE, 1 hit
InterProiView protein in InterPro
IPR023030 Bifunc_HldE
IPR002173 Carboh/pur_kinase_PfkB_CS
IPR004821 Cyt_trans-like
IPR011611 PfkB_dom
IPR011913 RfaE_dom_I
IPR011914 RfaE_dom_II
IPR029056 Ribokinase-like
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF01467 CTP_transf_like, 1 hit
PF00294 PfkB, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00125 cyt_tran_rel, 1 hit
TIGR02198 rfaE_dom_I, 1 hit
TIGR02199 rfaE_dom_II, 1 hit
PROSITEiView protein in PROSITE
PS00583 PFKB_KINASES_1, 1 hit

Sequencei

Sequence statusi: Complete.

A4YYB6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFDFDDLSQA IARQTVLCVG DLMLDEFVYG EVSRISPEAP APVIAVQRSE
60 70 80 90 100
TNIGGAGNVA RNIAALGARG IFVGLVGQDA AGDQLEAELA KVDGIESVLV
110 120 130 140 150
RDPSRPTTRK VRFVSEHFST HMLRADWERA APASEDIEQK LIAAILPLIA
160 170 180 190 200
RADIVLLSDY AKGVLTARVL RNVIDATRRA GKRVIVDPKS ANLAIYRGAS
210 220 230 240 250
VLTPNRKEFS EATRSRAETE QEIAAAAQDA IYLADCEAIL VTQSERGMTL
260 270 280 290 300
VPRQGDPIHV PAYPVKVRDV SGAGDTVVAV LAAALAAGAA WEDALRMASA
310 320 330 340 350
AAAVAVSKQG TASVTSAELR RKILPHAYRA AEEKIIENDG ELATRVAVWR
360 370 380 390 400
SEGLRVGFTN GCFDILHPGH VKVLTAARAA CDRLVVGLNS DTSVKRLKGE
410 420 430 440 450
SRPVQDERAR AEVLAALEAV DLVAIFTEET PLRLIELVRP SVLVKGGDYT
460 470 480 490
REQVVGHEVV EAAGGEVLLI DILKGHSTTA LVDRARSDQR
Length:490
Mass (Da):52,471
Last modified:May 29, 2007 - v1
Checksum:iC96E0AEF94C8B0F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU234118 Genomic DNA Translation: CAL78892.1
RefSeqiWP_012028828.1, NC_009445.1

Genome annotation databases

EnsemblBacteriaiCAL78892; CAL78892; BRADO5201
KEGGibra:BRADO5201

Similar proteinsi

Entry informationi

Entry nameiHLDE_BRASO
AccessioniPrimary (citable) accession number: A4YYB6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 29, 2007
Last modified: May 23, 2018
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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