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Protein

Bifunctional protein HldE

Gene

hldE

Organism
Bradyrhizobium sp. (strain ORS278)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7-phosphate at the C-1 position to selectively form D-glycero-beta-D-manno-heptose-1,7-bisphosphate.UniRule annotation
Catalyzes the ADP transfer from ATP to D-glycero-beta-D-manno-heptose 1-phosphate, yielding ADP-D-glycero-beta-D-manno-heptose.UniRule annotation

Catalytic activityi

D-glycero-beta-D-manno-heptose 7-phosphate + ATP = D-glycero-beta-D-manno-heptose 1,7-bisphosphate + ADP.UniRule annotation
D-glycero-beta-D-manno-heptose 1-phosphate + ATP = ADP-D-glycero-beta-D-manno-heptose + diphosphate.UniRule annotation

Pathwayi: ADP-L-glycero-beta-D-manno-heptose biosynthesis

This protein is involved in step 1 and 3 of the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Bifunctional protein HldE (hldE), Bifunctional protein HldE (BRADO5164)
  2. no protein annotated in this organism
  3. Bifunctional protein HldE (hldE), Bifunctional protein HldE (BRADO5164)
  4. ADP-L-glycero-D-manno-heptose-6-epimerase (rfaD)
This subpathway is part of the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate, the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei275 – 2751UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi205 – 2084ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywords - Molecular functioni

Kinase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSP114615:GJN5-4966-MONOMER.
UniPathwayiUPA00356; UER00437.
UPA00356; UER00439.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein HldEUniRule annotation
Including the following 2 domains:
D-beta-D-heptose 7-phosphate kinaseUniRule annotation (EC:2.7.1.167UniRule annotation)
Alternative name(s):
D-beta-D-heptose 7-phosphotransferaseUniRule annotation
D-glycero-beta-D-manno-heptose-7-phosphate kinaseUniRule annotation
D-beta-D-heptose 1-phosphate adenylyltransferaseUniRule annotation (EC:2.7.7.70UniRule annotation)
Alternative name(s):
D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferaseUniRule annotation
Gene namesi
Name:hldEUniRule annotation
Ordered Locus Names:BRADO5201
OrganismiBradyrhizobium sp. (strain ORS278)
Taxonomic identifieri114615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000001994 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Bifunctional protein HldEPRO_1000069398Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi114615.BRADO5201.

Structurei

3D structure databases

ProteinModelPortaliA4YYB6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 330330RibokinaseAdd
BLAST
Regioni358 – 490133CytidylyltransferaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the carbohydrate kinase PfkB family.UniRule annotation
In the C-terminal section; belongs to the cytidylyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DW0. Bacteria.
COG2870. LUCA.
HOGENOMiHOG000237584.
KOiK03272.
OMAiEACYLAN.
OrthoDBiEOG68Q0W4.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_01603. HldE.
InterProiIPR023030. Bifunc_HldE.
IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR004821. Cyt_trans-like.
IPR011611. PfkB_dom.
IPR011913. RfaE_dom_I.
IPR011914. RfaE_dom_II.
IPR029056. Ribokinase-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
PF00294. PfkB. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.
TIGR02198. rfaE_dom_I. 1 hit.
TIGR02199. rfaE_dom_II. 1 hit.
PROSITEiPS00583. PFKB_KINASES_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4YYB6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFDFDDLSQA IARQTVLCVG DLMLDEFVYG EVSRISPEAP APVIAVQRSE
60 70 80 90 100
TNIGGAGNVA RNIAALGARG IFVGLVGQDA AGDQLEAELA KVDGIESVLV
110 120 130 140 150
RDPSRPTTRK VRFVSEHFST HMLRADWERA APASEDIEQK LIAAILPLIA
160 170 180 190 200
RADIVLLSDY AKGVLTARVL RNVIDATRRA GKRVIVDPKS ANLAIYRGAS
210 220 230 240 250
VLTPNRKEFS EATRSRAETE QEIAAAAQDA IYLADCEAIL VTQSERGMTL
260 270 280 290 300
VPRQGDPIHV PAYPVKVRDV SGAGDTVVAV LAAALAAGAA WEDALRMASA
310 320 330 340 350
AAAVAVSKQG TASVTSAELR RKILPHAYRA AEEKIIENDG ELATRVAVWR
360 370 380 390 400
SEGLRVGFTN GCFDILHPGH VKVLTAARAA CDRLVVGLNS DTSVKRLKGE
410 420 430 440 450
SRPVQDERAR AEVLAALEAV DLVAIFTEET PLRLIELVRP SVLVKGGDYT
460 470 480 490
REQVVGHEVV EAAGGEVLLI DILKGHSTTA LVDRARSDQR
Length:490
Mass (Da):52,471
Last modified:May 29, 2007 - v1
Checksum:iC96E0AEF94C8B0F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU234118 Genomic DNA. Translation: CAL78892.1.
RefSeqiWP_012028828.1. NC_009445.1.

Genome annotation databases

EnsemblBacteriaiCAL78892; CAL78892; BRADO5201.
KEGGibra:BRADO5201.
PATRICi21226389. VBIBraSp122330_4992.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU234118 Genomic DNA. Translation: CAL78892.1.
RefSeqiWP_012028828.1. NC_009445.1.

3D structure databases

ProteinModelPortaliA4YYB6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi114615.BRADO5201.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL78892; CAL78892; BRADO5201.
KEGGibra:BRADO5201.
PATRICi21226389. VBIBraSp122330_4992.

Phylogenomic databases

eggNOGiENOG4105DW0. Bacteria.
COG2870. LUCA.
HOGENOMiHOG000237584.
KOiK03272.
OMAiEACYLAN.
OrthoDBiEOG68Q0W4.

Enzyme and pathway databases

UniPathwayiUPA00356; UER00437.
UPA00356; UER00439.
BioCyciBSP114615:GJN5-4966-MONOMER.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_01603. HldE.
InterProiIPR023030. Bifunc_HldE.
IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR004821. Cyt_trans-like.
IPR011611. PfkB_dom.
IPR011913. RfaE_dom_I.
IPR011914. RfaE_dom_II.
IPR029056. Ribokinase-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
PF00294. PfkB. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.
TIGR02198. rfaE_dom_I. 1 hit.
TIGR02199. rfaE_dom_II. 1 hit.
PROSITEiPS00583. PFKB_KINASES_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ORS278.

Entry informationi

Entry nameiHLDE_BRASO
AccessioniPrimary (citable) accession number: A4YYB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 29, 2007
Last modified: November 11, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.