A4YYA7 (CYSD_BRASO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfate adenylyltransferase subunit 2
EC=2.7.7.4

Alternative name(s):
ATP-sulfurylase small subunit
Sulfate adenylate transferase
Short name=SAT

Gene names

Name:	cysD
Ordered Locus Names:	BRADO5192

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP MF_00064
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP MF_00064
Subunit structure	Heterodimer composed of CysD, the smaller subunit, and CysN By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysD subfamily.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate reduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Sulfate adenylyltransferase subunit 2 HAMAP MF_00064		PRO_0000340181

Sequences

	Sequence		Length	Mass (Da)
	A4YYA7 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: D2A3F312AC12AFCE	FASTA	310	35,770
	10 20 30 40 50 60 MTMDQVPISR SQLPAHLKRL ESESIEIIRE VAAELKNPVM LYSIGKDSGV MLHIAFKAFF 70 80 90 100 110 120 PGRLPFPLLH VDTTWKFRDM IMFRDELAKK LGFDLLVHTN EDGLRAGINP FTSGPSLHTH 130 140 150 160 170 180 VMKTEALKQA LDLHGFDAAF GGARRDEEKS RAKERIFSFR AEGHVWEPRN QRPELWNLFN 190 200 210 220 230 240 TRIRPGESLR VFPLSNWTEF DVWHYISLER IPVVPLYFAK ERPVVWRDGT WIMVDDARFP 250 260 270 280 290 300 LAPGETPQLR KVRFRTLGCY PLTGAIESAA TSVEDILDEM RTTRISERQG RLIDTDEAAS 310 MEKKKKEGYF

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ORS278.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU234118 Genomic DNA. Translation: CAL78883.1.
RefSeq	YP_001207100.1. NC_009445.1.
3D structure databases
ProteinModelPortal	A4YYA7.
SMR	A4YYA7. Positions 16-219.
ModBase	Search...
Protein-protein interaction databases
STRING	A4YYA7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5116117.
GenomeReviews	Gene locus BRADO5192 in contig CU234118_GR.
KEGG	bra:BRADO5192.
PATRIC	21226377. VBIBraSp122330_4986.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0175.
HOGENOM	HBG758022.
OMA	NARIHPG.
ProtClustDB	PRK12563.
Enzyme and pathway databases
BioCyc	BSP376:BRADO5192-MONOMER.
Family and domain databases
HAMAP	MF_00064. Sulf_adenylyltr_sub2. [Tree]
InterPro	IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. IPR011784. SO4_adenylTrfase_ssu. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K00957.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMs	TIGR02039. CysD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSD_BRASO

Accession

Primary (citable) accession number: A4YYA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	May 29, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program