Reviewed, UniProtKB/Swiss-Prot A4YWG4 (PYRB_BRASO)

Names and origin

Protein names

Recommended name:
    Aspartate carbamoyltransferase
    EC=2.1.3.2
Alternative name(s):
    Aspartate transcarbamylase
      Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	BRADO4502

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 2/6.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Aspartate carbamoyltransferase		PRO_0000301558

Sequences

	Sequence		Length	Mass (Da)
	A4YWG4-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 6B492193A74C6834	FASTA	317	34,452
	10 20 30 40 50 60 MATSPKSTFV LGHRHLLGIE GLSTADITGL LDLSEEYVEL NRQVDKKRTV LRGRTQVNLF 70 80 90 100 110 120 FEASTRTQSS FELAGKRLGA DVMNMSVSSS SIKKGETLID TAVTLNAMHP DILVVRHHAS 130 140 150 160 170 180 GAVELLARKV DGSVINAGDG AHEHPTQALL DALTIRRNKG RIEGLVIAIC GDVLHSRVAR 190 200 210 220 230 240 SNILLLNTMG ARVRVVAPST LLPPGIERMG VEVARDMREG LNGADIVMML RLQRERMNGS 250 260 270 280 290 300 FVPSSSEYFQ YFGLDQKKLA YAKPDALVMH PGPMNRGVEI DSIVADGAQS LIREQVEMGV 310 AVRMAVLEAL ARNLPNA

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU234118 Genomic DNA. Translation: CAL78240.1.
RefSeq	YP_001206460.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5115088.
GenomeReviews	Gene locus BRADO4502 in contig CU234118_GR.
KEGG	bra:BRADO4502.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00001. [Tree]
InterPro	IPR006130. Asp/Orn_carbamoyltranf. IPR006132. Asp/Orn_carbamoyltranf_P_bd. IPR006131. Asp_carbamoyltransf_Asp/Orn_bd. IPR002082. Aspartate_carbamoyltransf_euk. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
TIGRFAMs	TIGR00670. asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_BRASO

Accession

Primary (citable) accession number: A4YWG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	May 29, 2007
Last modified:	November 25, 2008
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)