A4YVG0 (DXR_BRASO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Short name=DXP reductoisomerase
EC=1.1.1.267

Alternative name(s):
1-deoxyxylulose-5-phosphate reductoisomerase
2-C-methyl-D-erythritol 4-phosphate synthase

Gene names

Name:	dxr
Ordered Locus Names:	BRADO4134

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00183
Catalytic activity	2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH. HAMAP MF_00183
Cofactor	Divalent cation By similarity. HAMAP MF_00183
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6. HAMAP MF_00183
Sequence similarities	Belongs to the DXR family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate reductoisomerase activity Inferred from electronic annotation. Source: EC NADPH binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	1-deoxy-D-xylulose 5-phosphate reductoisomerase HAMAP MF_00183		PRO_1000020222
Regions
	Nucleotide binding	22 – 51	30	NADP By similarity
Sites
	Metal binding	162	1	Divalent metal cation By similarity
	Metal binding	164	1	Divalent metal cation By similarity
	Metal binding	233	1	Divalent metal cation By similarity
	Binding site	137	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	188	1	Substrate By similarity
	Binding site	211	1	Substrate By similarity
	Binding site	233	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4YVG0 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 34785CF1C06F58D3	FASTA	407	42,811
	10 20 30 40 50 60 MSAVPLRNNK AIAADVRSIT VLGATGSIGD STMDLLRGAP ERYRVEALTG NSNIEGLAKL 70 80 90 100 110 120 AREFNSRFVA VADPARLGEL REALAGTGIA CGAGESAIIE AAARPADWVM AAVSGAAGLK 130 140 150 160 170 180 PALAAVDRGA TVALANKECL VCAGDFFMQR AAQAGACILP ADSEHNALFQ ALSSGNREEL 190 200 210 220 230 240 VRVIITASGG PFRTWAPQDI EQATLEQALK HPNWSMGQKI TIDSASMMNK GLEVIEASYL 250 260 270 280 290 300 FALSPDEIDV LVHPQSIVHG MVEFSDRSVV AQLGAPDMRI PIAHCLGWPE RIKGPSARLD 310 320 330 340 350 360 LAKIGTLTFE APDFERFPGL RLAYDSLRAG RGATTVFNAA NEVAVAAFIG GHIRFGAIAR 370 380 390 400 LVEATLNDWT RSGNQAPLSS ADDAIAIDHD ARKRAAGLLP QIAAKAS

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ORS278.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU234118 Genomic DNA. Translation: CAL77886.1.
RefSeq	YP_001206111.1. NC_009445.1.
3D structure databases
ProteinModelPortal	A4YVG0.
ModBase	Search...
Protein-protein interaction databases
STRING	A4YVG0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5118770.
GenomeReviews	Gene locus BRADO4134 in contig CU234118_GR.
KEGG	bra:BRADO4134.
PATRIC	21224351. VBIBraSp122330_3983.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0743.
HOGENOM	HBG430762.
OMA	IHSMVEY.
ProtClustDB	PRK05447.
Enzyme and pathway databases
BioCyc	BSP376:BRADO4134-MONOMER.
Family and domain databases
HAMAP	MF_00183. DXP_reductoisom. [Tree]
InterPro	IPR003821. DXP_reductoisomerase. IPR013644. DXP_reductoisomerase_C. IPR013512. DXP_reductoisomerase_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00099.
Pfam	PF08436. DXP_redisom_C. 1 hit. PF02670. DXP_reductoisom. 1 hit. [Graphical view]
PIRSF	PIRSF006205. Dxp_reductismrs. 1 hit.
TIGRFAMs	TIGR00243. Dxr. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DXR_BRASO

Accession

Primary (citable) accession number: A4YVG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 29, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program