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A4YVF1

- SYE_BRASO

UniProt

A4YVF1 - SYE_BRASO

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Bradyrhizobium sp. (strain ORS278)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (29 May 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei247 – 2471ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBSP114615:GJN5-3937-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:BRADO4125
    OrganismiBradyrhizobium sp. (strain ORS278)
    Taxonomic identifieri114615 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
    ProteomesiUP000001994: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 478478Glutamate--tRNA ligasePRO_0000330956Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi114615.BRADO4125.

    Structurei

    3D structure databases

    ProteinModelPortaliA4YVF1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi15 – 2511"HIGH" regionAdd
    BLAST
    Motifi244 – 2485"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252722.
    KOiK01885.
    OMAiAMGWEVP.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4YVF1-1 [UniParc]FASTAAdd to Basket

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    MTSSTTSSIV TRFAPSPTGF LHIGGARTAL FNWLYARGRG GKMLLRIEDT    50
    DRERSTTAAI DAILDGLKWL ELDWDGEVIY QYSRAARHRE VAEQLLATGM 100
    AYRCYATAEE LVAMREKARA EGRTRLYDGM WRDRDPKEAP EGVKPTIRLR 150
    APLTGETMVE DQVQGRVVWQ NENLDDLVLL RGDGNPTYML AVVVDDHDMG 200
    VTHVIRGDDH LINAARQKQI YDALGWTVPS MSHIPLIHGP DGSKLSKRHG 250
    ALGVDAYRAM GYLPSALRNY LVRLGWSHGD QEIFSTEEMI KAFDLPAIGR 300
    SAARFDFAKL ENLNGHYIRH SDDAELVRQF EDVLNYVPNG AALKAKLNDA 350
    TRAQLTQAMP SLKERAKTLL ELIDGAAFIF ADRPLPIDAK AAALLTPESR 400
    ALIGRLHAAL SAVEPWSGPA TEAALRAFAE ANNLKLGAVA QPLRAALTGR 450
    TTSPGIFDVL AILGRDESLG RLKDQAIA 478
    Length:478
    Mass (Da):52,642
    Last modified:May 29, 2007 - v1
    Checksum:iEB930704C9CB83AF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU234118 Genomic DNA. Translation: CAL77877.1.
    RefSeqiWP_011927006.1. NC_009445.1.
    YP_001206102.1. NC_009445.1.

    Genome annotation databases

    EnsemblBacteriaiCAL77877; CAL77877; BRADO4125.
    GeneIDi5121004.
    KEGGibra:BRADO4125.
    PATRICi21224333. VBIBraSp122330_3974.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU234118 Genomic DNA. Translation: CAL77877.1 .
    RefSeqi WP_011927006.1. NC_009445.1.
    YP_001206102.1. NC_009445.1.

    3D structure databases

    ProteinModelPortali A4YVF1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 114615.BRADO4125.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAL77877 ; CAL77877 ; BRADO4125 .
    GeneIDi 5121004.
    KEGGi bra:BRADO4125.
    PATRICi 21224333. VBIBraSp122330_3974.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252722.
    KOi K01885.
    OMAi AMGWEVP.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci BSP114615:GJN5-3937-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ORS278.

    Entry informationi

    Entry nameiSYE_BRASO
    AccessioniPrimary (citable) accession number: A4YVF1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3