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A4YVF1 (SYE_BRASO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:BRADO4125
OrganismBradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]
Taxonomic identifier114615 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 478478Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000330956

Regions

Motif15 – 2511"HIGH" region HAMAP-Rule MF_00022
Motif244 – 2485"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2471ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4YVF1 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: EB930704C9CB83AF

FASTA47852,642
        10         20         30         40         50         60 
MTSSTTSSIV TRFAPSPTGF LHIGGARTAL FNWLYARGRG GKMLLRIEDT DRERSTTAAI 

        70         80         90        100        110        120 
DAILDGLKWL ELDWDGEVIY QYSRAARHRE VAEQLLATGM AYRCYATAEE LVAMREKARA 

       130        140        150        160        170        180 
EGRTRLYDGM WRDRDPKEAP EGVKPTIRLR APLTGETMVE DQVQGRVVWQ NENLDDLVLL 

       190        200        210        220        230        240 
RGDGNPTYML AVVVDDHDMG VTHVIRGDDH LINAARQKQI YDALGWTVPS MSHIPLIHGP 

       250        260        270        280        290        300 
DGSKLSKRHG ALGVDAYRAM GYLPSALRNY LVRLGWSHGD QEIFSTEEMI KAFDLPAIGR 

       310        320        330        340        350        360 
SAARFDFAKL ENLNGHYIRH SDDAELVRQF EDVLNYVPNG AALKAKLNDA TRAQLTQAMP 

       370        380        390        400        410        420 
SLKERAKTLL ELIDGAAFIF ADRPLPIDAK AAALLTPESR ALIGRLHAAL SAVEPWSGPA 

       430        440        450        460        470 
TEAALRAFAE ANNLKLGAVA QPLRAALTGR TTSPGIFDVL AILGRDESLG RLKDQAIA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU234118 Genomic DNA. Translation: CAL77877.1.
RefSeqYP_001206102.1. NC_009445.1.

3D structure databases

ProteinModelPortalA4YVF1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING114615.BRADO4125.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL77877; CAL77877; BRADO4125.
GeneID5121004.
KEGGbra:BRADO4125.
PATRIC21224333. VBIBraSp122330_3974.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252722.
KOK01885.
OMAAMGWEVP.
OrthoDBEOG6DRPF7.
ProtClustDBPRK01406.

Enzyme and pathway databases

BioCycBSP114615:GJN5-3937-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_BRASO
AccessionPrimary (citable) accession number: A4YVF1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 29, 2007
Last modified: February 19, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries