Reviewed, UniProtKB/Swiss-Prot A4YVC7 (KDSA_BRASO)

Names and origin

Protein names

Recommended name:
    2-dehydro-3-deoxyphosphooctonate aldolase
    EC=2.5.1.55
Alternative name(s):
    Phospho-2-dehydro-3-deoxyoctonate aldolase
    3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase
    KDO-8-phosphate synthetase
      Short name=KDO 8-P synthase
      Short name=KDOPS

Gene names

Name:	kdsA
Ordered Locus Names:	BRADO4101

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity HAMAP MF_00056.
Sequence similarities	Belongs to the kdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	2-dehydro-3-deoxyphosphooctonate aldolase HAMAP MF_00056		PRO_0000304434

Sequences

	Sequence		Length	Mass (Da)
	A4YVC7-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 4FC832A4B31E912A	FASTA	287	29,684
	10 20 30 40 50 60 MNAPISAAPV VTAGSVRFGN QLPLAVIAGP CQLESRGHAL EVASALKEIA TRLNISLVYK 70 80 90 100 110 120 TSFDKANRTS GSAARGLGLA QSLPIFAEIR SSLGLPVLTD VHDASQCAEV AQAVDILQIP 130 140 150 160 170 180 AFLCRQTDLL LAAAATGKVV NVKKGQFLAP WDMANVVAKI TGAGNPNVLA TERGVSFGYN 190 200 210 220 230 240 TLISDMRSLP IMAKTTGAPV IFDATHSVQQ PGGQGTSSGG QREFVPVLAR AAVAVGVAGV 250 260 270 280 FIETHPDPDH APSDGPNMVP LAQFEGLLRT LMAFDALAKG QAADAIR

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU234118 Genomic DNA. Translation: CAL77853.1.
RefSeq	YP_001206078.1.
3D structure databases
SMR	A4YVC7. Positions 23-279.
ModBase	Search...
Protein-protein interaction databases
STRING	A4YVC7.
Genome annotation databases
GeneID	5121237.
GenomeReviews	Gene locus BRADO4101 in contig CU234118_GR.
KEGG	bra:BRADO4101.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2877.
HOGENOM	HBG575990.
OMA	LAPWDMG.
Family and domain databases
HAMAP	MF_00056. KDO8P_synth. [Tree]
InterPro	IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_BRASO

Accession

Primary (citable) accession number: A4YVC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	May 29, 2007
Last modified:	February 9, 2010
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)