Reviewed,
UniProtKB/Swiss-Prot A4YUQ7 (ISPDF_BRASO)
Last modified
February 9, 2010.
Version 18.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (2) |
 Third-party data |
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Names and origin
| Protein names | Recommended name: Bifunctional enzyme ispD/ispF Including the following 2 domains: 1- Recommended name: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase EC=2.7.7.60 Alternative name(s): 4-diphosphocytidyl-2C-methyl-D-erythritol synthase MEP cytidylyltransferase Short name=MCT 2- Recommended name: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase Short name=MECPS Short name=MECDP-synthase EC=4.6.1.12 | ||||
| Gene names |
| ||||
| Organism | Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 114615 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium |
Protein attributes
| Sequence length | 393 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (ispD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (ispF) By similarity. HAMAP MF_01520 |
| Catalytic activity | CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_01520 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_01520 |
| Cofactor | Divalent metal cations By similarity. HAMAP MF_01520 |
| Pathway | Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_01520 |
| Sequence similarities | In the N-terminal section; belongs to the ispD family. In the C-terminal section; belongs to the ispF family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Isoprene biosynthesis |
| Ligand | Metal-binding |
| Molecular function | Lyase Nucleotidyltransferase Transferase |
| Technical term | Complete proteome Multifunctional enzyme |
| Gene Ontology (GO) | |
| Biological process | terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP |
| Molecular function | 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity Inferred from electronic annotation. Source: HAMAP 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activityInferred from electronic annotation. Source: HAMAP metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 393 | 393 | Bifunctional enzyme ispD/ispF HAMAP MF_01520 | PRO_0000296740 | |||||
Regions | |||||||||
| Region | 1 – 234 | 234 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_01520 | ||||||
| Region | 235 – 393 | 159 | 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_01520 | ||||||
Sites | |||||||||
| Metal binding | 241 | 1 | Divalent metal cation By similarity | ||||||
| Metal binding | 243 | 1 | Divalent metal cation By similarity | ||||||
| Metal binding | 275 | 1 | Divalent metal cation By similarity | ||||||
| Site | 19 | 1 | Transition state stabilizer By similarity | ||||||
| Site | 26 | 1 | Transition state stabilizer By similarity | ||||||
| Site | 156 | 1 | Positions MEP for the nucleophilic attack By similarity | ||||||
| Site | 213 | 1 | Positions MEP for the nucleophilic attack By similarity | ||||||
| Site | 267 | 1 | Transition state stabilizer By similarity | ||||||
| Site | 366 | 1 | Transition state stabilizer By similarity | ||||||
Sequences
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References
| [1] | "Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z.  Sadowsky M.Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | CU234118 Genomic DNA. Translation: CAL77633.1. |
| RefSeq | YP_001205858.1. |
3D structure databases | |
| SMR | A4YUQ7. Positions 6-392. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | A4YUQ7. |
Genome annotation databases | |
| GeneID | 5117686. |
| GenomeReviews | Gene locus BRADO3869 in contig CU234118_GR. |
| KEGG | bra:BRADO3869. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| eggNOG | COG1211. |
| HOGENOM | HBG672839. |
| OMA | IVLIHDA. |
Family and domain databases | |
| HAMAP | MF_01520. IspDF. [Tree] |
| InterPro | IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. IPR003526. MECDP_synthase_core. IPR020555. MECDP_synthase_CS. [Graphical view] |
| Pfam | PF01128. IspD. 1 hit. PF02542. YgbB. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00453. ispD. 1 hit. TIGR00151. ispF. 1 hit. |
| PROSITE | PS01295. ISPD. 1 hit. PS01350. ISPF. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | ISPDF_BRASO | ||||||||
| Accession | Primary (citable) accession number: A4YUQ7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
