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Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Bradyrhizobium sp. (strain ORS 278)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotation

Catalytic activityi

[Glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotation
ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:BRADO2744
OrganismiBradyrhizobium sp. (strain ORS 278)
Taxonomic identifieri114615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000001994 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002129841 – 986Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeAdd BLAST986

Interactioni

Protein-protein interaction databases

STRINGi114615.BRADO2744.

Structurei

3D structure databases

SMRiA4YRN0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 473Adenylyl removaseUniRule annotationAdd BLAST473
Regioni478 – 986Adenylyl transferaseUniRule annotationAdd BLAST509

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000256492.
KOiK00982.
OMAiFTVQLLQ.
OrthoDBiPOG091H0675.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.

Sequencei

Sequence statusi: Complete.

A4YRN0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSSAPGNAD GQSRLAARFV AGPHVRAPDT AEHRLQDWLA ELEPSLAASL
60 70 80 90 100
RDQFASGWAR SILLGIVESS PYLFDLIRAD AQRLDRLLRC DPQTHLAELI
110 120 130 140 150
ARTGREVFAC SGEADVMSLL RRLKSEAALL IALCDIGGVW PVMQVTAALT
160 170 180 190 200
DVAVAAVQMA LRHLLRQEAA RGRLAPIDPA QPELGCGLFV LAMGKMGAGE
210 220 230 240 250
LNYSSDIDLI VFFDPDATTL AHDIEPQPFF VRVTQALARL LQSRTADGYV
260 270 280 290 300
FRVDLRLRPD PASTQVAMST EAALHYYERE GRTWERAAMI KARICAGDVA
310 320 330 340 350
AGEAMLAELS PFVWRKHLDF QALTDVHDMK RQMQVYRGHS EIAVEGHNVK
360 370 380 390 400
VGRGGIREIE FFAQTQQLIA GGRHPELRVR PTLQALDVLT ASNWITAQAR
410 420 430 440 450
DELTSAYEFL RRVEHRLQMM ADEQIHSLPD DVDGVARFAC FFGYESRERF
460 470 480 490 500
ATDLLFHLDI VQGHYARLFE GDPTGTVSLP PVNYGAGPDE PRLLEHLVAL
510 520 530 540 550
GFRDPMMVAL TLQQWLAGDY RVFRAEATRT TFNEFLPALI DGLAHADEPD
560 570 580 590 600
RAVVAFDRFL QALQLGGRLI TLLGQNRDLV ALVALVLGAA PRLGDMLARQ
610 620 630 640 650
PRLMDGLIDP RFFGAIPDKR ELSTRLAATL QDAGSYEEFL DRLRLFGQES
660 670 680 690 700
LFLIGTRILS GTVSAQQAGT AFADVAEGIV HTVHNLVIER FAAQHGRIRG
710 720 730 740 750
QETAIIAMGR LGAREMTASS DLDLILLYDF DADAPDSDGE RPLQGAHYFA
760 770 780 790 800
RFTQRLISAF TSRTNYGVLY DIDMRLRPSG RAGPLASHID SFAHYQEHEA
810 820 830 840 850
WTWEHMALTR ARVISASPAF RARIEAIIQT ALRRSRDAQA IARDVADMRR
860 870 880 890 900
AIAAEKGETD LWDLKHAAGG MVDIDFVAQY LQLVHAASKP EILDISSLQV
910 920 930 940 950
LDHAERLGVL PRADAVILRH AARMYHDLTQ ILRLCVSDKF KPDQAGDDLL
960 970 980
RVMTRAGDAP DFSALEARVR ETQSEVRRVF TALLER
Length:986
Mass (Da):109,423
Last modified:May 29, 2007 - v1
Checksum:iECE27568ECEA14EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU234118 Genomic DNA. Translation: CAL76556.1.
RefSeqiWP_011925761.1. NC_009445.1.

Genome annotation databases

EnsemblBacteriaiCAL76556; CAL76556; BRADO2744.
KEGGibra:BRADO2744.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLNE_BRASO
AccessioniPrimary (citable) accession number: A4YRN0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 29, 2007
Last modified: June 7, 2017
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families