Reviewed, UniProtKB/Swiss-Prot A4YRD9 (DNAE2_BRASO)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	BRADO2647

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	1170 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subcellular location	CytoplasmProbable.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: InterPro error-prone DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1170	1170	Error-prone DNA polymerase		PRO_1000070589

Sequences

	Sequence		Length	Mass (Da)
	A4YRD9-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 73C24A0B6EA41E32	FASTA	1,170	131,983
	10 20 30 40 50 60 MTPIPYAEIG VTTNFSFLHG GSHPQAYVHQ AAEYGLTAIG IADHNTLAGI VRAYSELSND 70 80 90 100 110 120 QLGYRPKLLY GARLVFTCGT PDILVYPRDR AAYGRLCQLL TRGKRGSDLD KVAKGDCRLA 130 140 150 160 170 180 FEDLFDFIAG QLLVLMPPHR FDEDALGAIL ARLKDSPAEG VWLAGSLLYR GDDRRRLARL 190 200 210 220 230 240 ARLAASAKVP LIATNDVLYH HPQQRMLQDV LTCIREKASI ESIGRRLQAN AERHLKPGHE 250 260 270 280 290 300 MARLFRDHPE AIAETLRFTD RIVFSLDQLK YQYPDEPVPP GKTAQGHLED LTWAGAKTYF 310 320 330 340 350 360 GDKLDDRLRA VLNKELALIA ELNYAHYFLT VHDIVRYARS EGILCQGRGS AANSAVCYVL 370 380 390 400 410 420 GITSVDPTKI DLLFERFISK ERLEPPDIDV DFEHSRREEV MQYVYHRYGR HRAAIIATVI 430 440 450 460 470 480 HYRPRSAIRD VGKALGLTED VTSVLADTVW GSWGDGLSDM QVRQAGLDPH NPMIRRAVEL 490 500 510 520 530 540 ASELITFPRH LSQHVGGYVL TQDRLDSYVP IGNAAMDDRT FIEWDKDDVD ALSMMKVDVL 550 560 570 580 590 600 ALGMLTCIRK SFDLIADHKG RRYVLSDIKS EDDNEVYQML QRGESLGVFQ VESRAQMNML 610 620 630 640 650 660 PRLKPRTFYD LVIEVAIVRP GPIQGDMVHP YLRRRNKLEK VTYPSPSPDH GPSDELYKVL 670 680 690 700 710 720 HKTLGVPLFQ EQAMRIAIEA AKFSPEEANG LRRAMATFRN VGTIGSYEEK MVSNMIARGY 730 740 750 760 770 780 DPAFAKSCFD QIKGFGSYGF PESHAASFAQ LVYVSSWLKY FHPDAFCCAL LNSQPMGFYA 790 800 810 820 830 840 PAQIVGDARK NGVEIRDIDV SHSFADNTLE KTDGEYCAVR LGFRQIDGFR WIDRDEERIR 850 860 870 880 890 900 KLSEAVRKEA LSVSTRSLPA ISPVSFRGAR SANPESRDSG FALRAPRNDN DRQIPLHNDD 910 920 930 940 950 960 REDDWADRII RARQRRPFTS LEDFARDTAL PKRALLLLAD ADAFRSLGLD RRAALWAVRR 970 980 990 1000 1010 1020 LPDDVPLPLF EAAIAREQPD EGAQPLPEMP LPEHVVADYQ TIRLSLKGHP MEFLRRRFAA 1030 1040 1050 1060 1070 1080 EGVLACRDVN DTNDRRRIRC AGVVLVRQRP GSAKGVVFMT LEDETGIANI VVWPKVMETF 1090 1100 1110 1120 1130 1140 RKEVMGARLV LVEGRIQSSP EKVVHLVAER LVDRTADLTL LSDDRLDAIP HGATPAEPLN 1150 1160 1170 DDRRDHTDNP SQRVSHPRNV RILPRSRDFH

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU234118 Genomic DNA. Translation: CAL76465.1.
RefSeq	YP_001204702.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5120654.
GenomeReviews	Gene locus BRADO2647 in contig CU234118_GR.
KEGG	bra:BRADO2647.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01902. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. PHP_N. IPR004805. PolC_alpha. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_BRASO

Accession

Primary (citable) accession number: A4YRD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 29, 2007
Last modified:	November 25, 2008
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)