Reviewed, UniProtKB/Swiss-Prot A4YQD3 (RBL1B_BRASO)

Names and origin

Protein names

Recommended name:
    Ribulose bisphosphate carboxylase large chain 2
      Short name=RuBisCO large subunit 2
    EC=4.1.1.39

Gene names

Name:	cbbL2
Ordered Locus Names:	BRADO2274

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site By similarity.
Catalytic activity	2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. HAMAP MF_01338 3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2. HAMAP MF_01338
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subunit structure	Heterohexadecamer of 8 large chains and 8 small chains By similarity.
Miscellaneous	The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel" By similarity.
Sequence similarities	Belongs to the RuBisCO large chain family. Type I subfamily.

Ontologies

Keywords
Biological process	Calvin cycle Carbon dioxide fixation Photosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Lyase Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW ribulose-bisphosphate carboxylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	Ribulose bisphosphate carboxylase large chain 2 HAMAP MF_01338		PRO_0000299962
Sites
	Active site	168	1	Proton acceptor By similarity
	Active site	287	1	Proton acceptor By similarity
	Metal binding	194	1	Magnesium; via carbamate group By similarity
	Metal binding	196	1	Magnesium By similarity
	Metal binding	197	1	Magnesium By similarity
	Binding site	116	1	Substrate; in homodimeric partner By similarity
	Binding site	166	1	Substrate By similarity
	Binding site	170	1	Substrate By similarity
	Binding site	288	1	Substrate By similarity
	Binding site	320	1	Substrate By similarity
	Binding site	372	1	Substrate By similarity
	Site	327	1	Transition state stabilizer By similarity
Amino acid modifications
	Modified residue	194	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4YQD3-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 7518D7CC0AE30D6C	FASTA	479	53,334
	10 20 30 40 50 60 MAEKSYQAGV KEYRKTYWTP EYVPLDTDLL AVFKIVAQAG VPREEAAAAV AAESSTGTWT 70 80 90 100 110 120 TVWTDLLTDL DYYKGRAYRI EPVPGDDNAF YAFIAYPIDL FEEGSVVNVL TSLVGNVFGF 130 140 150 160 170 180 KAVRSLRLED IRFPLAYVKT CGGPPNGIQL ERDRLNKYGR PLLGCTIKPK LGLSAKNYGR 190 200 210 220 230 240 AVYECLRGGL DFTKDDENIN SQPFMRWQHR FEFVMEAVHK ATSETGERKG HYLNVTAPTP 250 260 270 280 290 300 EEMYKRAEFA KSLGAPIIMH DFLTAGFTAN TGLANWCREN GMLLHIHRAM HAVLDRNPMH 310 320 330 340 350 360 GIHFRVLTKC LRLSGGDHLH SGTVVGKLEG DREATIGWVD LMREPFVPEN RARGIFFDQD 370 380 390 400 410 420 WGAMPGVMPV ASGGIHVWHM PALTAIFGDD ACFQFGGGTL GHPWGNAAGA HANRVALEAC 430 440 450 460 470 VEARNQGRPV EREGREILTE AAQHSPELKI AMETWKEIKF EFDVVDKLDT GPMLRVVNA

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU234118 Genomic DNA. Translation: CAL76109.1.
RefSeq	YP_001204346.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5115889.
GenomeReviews	Gene locus BRADO2274 in contig CU234118_GR.
KEGG	bra:BRADO2274.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A4YQD3. YTPDYTP.
Family and domain databases
HAMAP	MF_01338. [Tree]
InterPro	IPR000685. RuBisCO_lsu_C. IPR017443. RuBisCO_lsu_fd_N. IPR017444. RuBisCO_lsu_N. [Graphical view]
Gene3D	G3DSA:3.20.20.110. RuBisCO_large. 1 hit. G3DSA:3.30.70.150. RuBisCO_large. 1 hit.
Pfam	PF00016. RuBisCO_large. 1 hit. PF02788. RuBisCO_large_N. 1 hit. [Graphical view]
PROSITE	PS00157. RUBISCO_LARGE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RBL1B_BRASO

Accession

Primary (citable) accession number: A4YQD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	May 29, 2007
Last modified:	June 16, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)