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A4YPQ3 (ISPE_BRASO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase
Gene names
Name:ispE
Ordered Locus Names:BRADO2022
OrganismBradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]
Taxonomic identifier114615 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length300 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061

Catalytic activity

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061

Sequence similarities

Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
   Biological processIsoprene biosynthesis
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processterpenoid biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Molecular function4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity

Inferred from electronic annotation. Source: EC

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3003004-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061
PRO_1000007816

Regions

Nucleotide binding102 – 11211ATP Potential

Sites

Active site171 By similarity
Active site1441 By similarity

Sequences

Sequence LengthMass (Da)Tools
A4YPQ3 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: D90C954BAD6B9032

FASTA30030,438
        10         20         30         40         50         60 
MLASTVTGAL QEQGRAKVNL TLRVVGRRAD GYHDLESVVA FADCADQLTL APSPELTLTT 

        70         80         90        100        110        120 
TGPLADACGD TSDNLVLKAA RLLAEAVPGL TLGAFTLEKV LPVAAGIGGG SADAAAALRL 

       130        140        150        160        170        180 
LARLNGLSLD DPRLQAVALK TGADVPVCVP SRACTMTGVG ENLQPLALPV LPCVMINPRV 

       190        200        210        220        230        240 
PVATKDVFQA LGLKPGDLLV GVSDVLAAPA WPKAGASIGD WVAALDQVKN DLEPPALKVQ 

       250        260        270        280        290        300 
PIIGTVLDAL RASNGVLLAR MSGSGATCFA IYGGDADAKI AGAAIAAAHP EWWVHAGTLS

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CU234118 Genomic DNA. Translation: CAL75879.1.
RefSeqYP_001204116.1. NC_009445.1.

3D structure databases

ProteinModelPortalA4YPQ3.
ModBaseSearch...

Protein-protein interaction databases

STRINGA4YPQ3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5116353.
GenomeReviewsGene locus BRADO2022 in contig CU234118_GR.
KEGGbra:BRADO2022.
PATRIC21220259. VBIBraSp122330_1954.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1947.
HOGENOMHBG734593.
OMACQLARMS.
ProtClustDBPRK14608.

Enzyme and pathway databases

BioCycBSP376:BRADO2022-MONOMER.

Family and domain databases

HAMAPMF_00061. IspE.
[Tree]
InterProIPR006204. GHMP_kinase.
IPR013750. GHMP_kinase_C.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
Gene3DG3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KOK00919.
PANTHERPTHR20861:SF2. IspE. 1 hit.
PfamPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFPIRSF010376. IspE. 1 hit.
SUPFAMSSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMsTIGR00154. IspE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameISPE_BRASO
AccessionPrimary (citable) accession number: A4YPQ3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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