A4YNR8 (KATG_BRASO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	BRADO1666

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	726 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 726	726	Catalase-peroxidase HAMAP MF_01961		PRO_0000354729
Sites
	Active site	91	1	Proton acceptor By similarity
	Metal binding	254	1	Iron (heme axial ligand) By similarity
	Site	87	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	90 ↔ 213		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-239) By similarity
	Cross-link	213 ↔ 239		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-90) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4YNR8 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 3FF741C9ADD72A16	FASTA	726	78,950
	10 20 30 40 50 60 MDDVSKCPFS GGLKGFKNKD WWPNQLDLSV LHQHSNLSDP LGEAFDYAKE FKSLDLDALV 70 80 90 100 110 120 KDLHALMTDS QEWWPADFGH YGPLFIRMAW HAAGTYRIGD GRGGAGTGQQ RFAPLNSWPD 130 140 150 160 170 180 NANLDKARRL LWPIKQKYGQ KISWADLFVL TGNVALESMG FKTFGFGGGR ADTWEPEQDI 190 200 210 220 230 240 YWGPEGKWLA DERYSGDREL AGSLAAVQMG LIYVNPEGPN GKPDPAAAAR DIRETFARMA 250 260 270 280 290 300 MNDEETVALI AGGHTFGKTH GAGDASLVGV EPEGADIAQQ GLGWASKYAS GKAGDTITSG 310 320 330 340 350 360 LEVIWTTTPT KWSNNYFENL FNYEWELTKS PAGAHQWTPK GGAGAGTVPD AHDPSKRHAP 370 380 390 400 410 420 SMLTTDIALR VDPAYEKISR RFLEHPDQFA DAFARAWFKL THRDMGPKAR YLGPLVPKEE 430 440 450 460 470 480 LIWQDPVPAL DHPVVDDKDV AALKAKILAA GLSVSQLVST AWASASTFRG SDKRGGANGA 490 500 510 520 530 540 RIRLAPQKDW AINNPAELSK VLAKLEGIQK EFNGSATGGK KVSLADLIVL AGNAGVEAAA 550 560 570 580 590 600 KKAGVEVTVP FAPGRTDASQ EQTDVESFAV LEPTHDGFRN YLSGKQWLSG EELLVDKAQL 610 620 630 640 650 660 LTLTAPETTV LVGGLRVLGA NAAGSKHGVF TTQPEVLSND FFVNLLDMGI AWTPVDQGEH 670 680 690 700 710 720 TFEGRDRKSG AVKWTATRAD LIFGSHSQLR ALAEVYASSD AKQKFVKDFV AAWTKVMNLD RFELKA

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ORS278.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU234118 Genomic DNA. Translation: CAL75544.1.
RefSeq	YP_001203781.1. NC_009445.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4YNR8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5118412.
GenomeReviews	Gene locus BRADO1666 in contig CU234118_GR.
KEGG	bra:BRADO1666.
PATRIC	21219557. VBIBraSp122330_1603.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	KRHAPSM.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	BSP376:BRADO1666-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_BRASO

Accession

Primary (citable) accession number: A4YNR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	May 29, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program