Reviewed, UniProtKB/Swiss-Prot A4YNR1 (RBL1A_BRASO)

Names and origin

Protein names

Recommended name:
    Ribulose bisphosphate carboxylase large chain 1
      Short name=RuBisCO large subunit 1
    EC=4.1.1.39

Gene names

Name:	cbbL1
Ordered Locus Names:	BRADO1659

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site By similarity.
Catalytic activity	2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. HAMAP MF_01338 3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2. HAMAP MF_01338
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subunit structure	Heterohexadecamer of 8 large chains and 8 small chains By similarity.
Miscellaneous	The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel" By similarity.
Sequence similarities	Belongs to the RuBisCO large chain family. Type I subfamily.

Ontologies

Keywords
Biological process	Calvin cycle Carbon dioxide fixation Photosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Lyase Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW ribulose-bisphosphate carboxylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	Ribulose bisphosphate carboxylase large chain 1 HAMAP MF_01338		PRO_0000299961
Sites
	Active site	177	1	Proton acceptor By similarity
	Active site	295	1	Proton acceptor By similarity
	Metal binding	203	1	Magnesium; via carbamate group By similarity
	Metal binding	205	1	Magnesium By similarity
	Metal binding	206	1	Magnesium By similarity
	Binding site	125	1	Substrate; in homodimeric partner By similarity
	Binding site	175	1	Substrate By similarity
	Binding site	179	1	Substrate By similarity
	Binding site	296	1	Substrate By similarity
	Binding site	328	1	Substrate By similarity
	Binding site	380	1	Substrate By similarity
	Site	335	1	Transition state stabilizer By similarity
Amino acid modifications
	Modified residue	203	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4YNR1-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: D969770506D00683	FASTA	486	53,965
	10 20 30 40 50 60 MNDQSITVRG KDRYKSGVME YKKMGYWEPS YQPNDTDVIA LFRVTPQDGV DPVEACAAVA 70 80 90 100 110 120 GESSTATWTV VWTDRLTAAE KYRAKCYRVE PVPGSPGSYF AYIAYDLDLF EPGSIANLTA 130 140 150 160 170 180 SIIGNVFGFK PLKALRLEDM RLPVAYVKTF QGPATGIVVE RERLDKFGRP LLGATVKPKL 190 200 210 220 230 240 GLSGRNYGRV VYEALKGGLD FTKDDENINS QPFMHWRERF LYCMEAVNKA QAATGEIKGT 250 260 270 280 290 300 YLNVTAATME DMYERAEFAK ELGSNIIMID LVIGYTAIQS MAKWSRKNDM ILHLHRAGHS 310 320 330 340 350 360 TYTRQRAHGV SFRVIAKWMR LAGVDHIHAG TVVGKLEGDP NTTRGYYDIC REDFNPMRLE 370 380 390 400 410 420 HGVFFDQHWA SLNKLMPVAS GGIHAGQMHQ LLDLLGEDVV LQFGGGTIGH PRGIAAGATA 430 440 450 460 470 480 NRVALEAMIL ARNEGRDYVH EGPEILAKAA MTCTPLREAL EIWKDVTFNY ESTDSPDFVP TVTPAA

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU234118 Genomic DNA. Translation: CAL75537.1.
RefSeq	YP_001203774.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5115881.
GenomeReviews	Gene locus BRADO1659 in contig CU234118_GR.
KEGG	bra:BRADO1659.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A4YNR1. INLHSAL.
Family and domain databases
HAMAP	MF_01338. [Tree]
InterPro	IPR000685. RuBisCO_lsu_C. IPR017443. RuBisCO_lsu_fd_N. IPR017444. RuBisCO_lsu_N. [Graphical view]
Gene3D	G3DSA:3.20.20.110. RuBisCO_large. 1 hit. G3DSA:3.30.70.150. RuBisCO_large. 1 hit.
Pfam	PF00016. RuBisCO_large. 1 hit. PF02788. RuBisCO_large_N. 1 hit. [Graphical view]
PROSITE	PS00157. RUBISCO_LARGE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RBL1A_BRASO

Accession

Primary (citable) accession number: A4YNR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	May 29, 2007
Last modified:	June 16, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)