Reviewed, UniProtKB/Swiss-Prot A4YNQ1 (ACSF_BRASO)

Names and origin

Protein names

Recommended name:
    Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase
      Short name=Aerobic Mg-protoporphyrin IX monomethyl ester oxidative cyclase
    EC=1.14.13.81

Gene names

Name:	acsF
Ordered Locus Names:	BRADO1649

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity.
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + H(2)O. 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + 2 H(2)O. 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = divinylprotochlorophyllide + NADP(+) + 2 H(2)O.
Cofactor	Iron By similarity.
Pathway	Porphyrin biosynthesis; bacteriochlorophyll biosynthesis (light-independent).
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Bacteriochlorophyll biosynthesis Chlorophyll biosynthesis Photosynthesis
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	bacteriochlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: HAMAP magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 353	353	Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase		PRO_1000070544

Sequences

	Sequence		Length	Mass (Da)
	A4YNQ1-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: F8A5BDD72FE1C2A7	FASTA	353	41,481
	10 20 30 40 50 60 MIAMEGGSGN ISTKMALEDT ILTPRFYTTD FAAMDRLNVD LVRREWDAVM AELRADHNRK 70 80 90 100 110 120 HFVRTPEFDK DLNELPEALR AEFKDFLVSS LTAEFSGCVL YAEIKKRITN PDIRELFQFM 130 140 150 160 170 180 SRDEARHAGF INEILKDHGI GVDLGFLTKT KKYTYFKPKF IFYATYLSEK IGYARYITIY 190 200 210 220 230 240 RQMERHPERQ FHPIFKWFER WCNDEFRHGE AFALLMRADP KLLSGYNKLW IKFFLLAVFA 250 260 270 280 290 300 TMHVRDHMRP AFYEALGMPP DEYDMRVFRI TSEISRQVFP VMLDIDNPRF WEGLKRLREI 310 320 330 340 350 SEAIAEAKAQ GGIMGTLKRA VLPLKAALTF GRLYMLPAKN NELPREIRLQ PAW

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU234118 Genomic DNA. Translation: CAL75527.1.
RefSeq	YP_001203764.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5118406.
GenomeReviews	Gene locus BRADO1649 in contig CU234118_GR.
KEGG	bra:BRADO1649.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01840. [Tree]
InterPro	IPR008434. AcsF. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACSF_BRASO

Accession

Primary (citable) accession number: A4YNQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 29, 2007
Last modified:	November 4, 2008
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)