Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Histidine ammonia-lyase

Gene

hutH

Organism
Bradyrhizobium sp. (strain ORS 278)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

L-histidine = urocanate + NH3.UniRule annotation

Pathwayi: L-histidine degradation into L-glutamate

This protein is involved in step 1 of the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Histidine ammonia-lyase (hutH)
  2. Urocanate hydratase (hutU)
  3. Imidazolonepropionase (hutI)
This subpathway is part of the pathway L-histidine degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine, the pathway L-histidine degradation into L-glutamate and in Amino-acid degradation.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Histidine metabolism

Enzyme and pathway databases

BioCyciBSP114615:GJN5-1525-MONOMER.
UniPathwayiUPA00379; UER00549.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine ammonia-lyaseUniRule annotation (EC:4.3.1.3UniRule annotation)
Short name:
HistidaseUniRule annotation
Gene namesi
Name:hutHUniRule annotation
Ordered Locus Names:BRADO1604
OrganismiBradyrhizobium sp. (strain ORS 278)
Taxonomic identifieri114615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000001994 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 515515Histidine ammonia-lyasePRO_1000021544Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki142 ↔ 1445-imidazolinone (Ala-Gly)UniRule annotation
Modified residuei143 – 14312,3-didehydroalanine (Ser)UniRule annotation

Post-translational modificationi

Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly.UniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi114615.BRADO1604.

Structurei

3D structure databases

ProteinModelPortaliA4YNK7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PAL/histidase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C84. Bacteria.
COG2986. LUCA.
HOGENOMiHOG000237620.
KOiK01745.
OMAiLILSHAC.
OrthoDBiPOG091H04Z2.

Family and domain databases

CDDicd00332. PAL-HAL. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00229. His_ammonia_lyase. 1 hit.
InterProiIPR001106. Aromatic_Lyase.
IPR024083. Fumarase/histidase_N.
IPR005921. HutH.
IPR008948. L-Aspartase-like.
IPR022313. Phe/His_NH3-lyase_AS.
[Graphical view]
PfamiPF00221. Lyase_aromatic. 1 hit.
[Graphical view]
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR01225. hutH. 1 hit.
PROSITEiPS00488. PAL_HISTIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4YNK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAIIAEPGH VSFDDLARVY AGAAIALGPA YWPRVEAAAA IVAKAAQGAE
60 70 80 90 100
PVYGINTGFG KLASKRIPPD QTALLQRNLI LSHCCGVGPA TPEPIVRLMI
110 120 130 140 150
ALKIISLGRG ASGVRRQIVD QLQAMLAQGV CPFVPQQGSV GASGDLAPLA
160 170 180 190 200
HMTAVMIGEG QAFVEGRLVP GREALAHVGL DPVTLGPKEG LALINGTQFS
210 220 230 240 250
TAYALAGLFR ARDLLNAALV TGALSVDAAM ASTAPFRPEI QALRGHPGQI
260 270 280 290 300
AAGRVLTELV DGSAIRLSHL EGDERVQDPY CLRCQPQVAG AALDLLTQAA
310 320 330 340 350
RTLVNEANAV TDNPLVLVET GEIISGGNFH AEPVAFAADQ IALALSELGA
360 370 380 390 400
ISERRIATLV DPALNFGLPP FLTPQPGLNS GFMIAEVTAA ALFAENKQRA
410 420 430 440 450
LPCSIDSTPT SANQEDHVSM AAHAARRLHD MADNLAHIIG IELLVAAQGI
460 470 480 490 500
ELRVPHGTSA ALSAVIGALR SHVPALESDR YMADDLAKAA ALVAGGALAR
510
AANVALGRDS FPRLG
Length:515
Mass (Da):53,267
Last modified:May 29, 2007 - v1
Checksum:i9C25FB1F4D3C8128
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU234118 Genomic DNA. Translation: CAL75483.1.
RefSeqiWP_011924714.1. NC_009445.1.

Genome annotation databases

EnsemblBacteriaiCAL75483; CAL75483; BRADO1604.
KEGGibra:BRADO1604.
PATRICi21219431. VBIBraSp122330_1540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU234118 Genomic DNA. Translation: CAL75483.1.
RefSeqiWP_011924714.1. NC_009445.1.

3D structure databases

ProteinModelPortaliA4YNK7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi114615.BRADO1604.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL75483; CAL75483; BRADO1604.
KEGGibra:BRADO1604.
PATRICi21219431. VBIBraSp122330_1540.

Phylogenomic databases

eggNOGiENOG4105C84. Bacteria.
COG2986. LUCA.
HOGENOMiHOG000237620.
KOiK01745.
OMAiLILSHAC.
OrthoDBiPOG091H04Z2.

Enzyme and pathway databases

UniPathwayiUPA00379; UER00549.
BioCyciBSP114615:GJN5-1525-MONOMER.

Family and domain databases

CDDicd00332. PAL-HAL. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00229. His_ammonia_lyase. 1 hit.
InterProiIPR001106. Aromatic_Lyase.
IPR024083. Fumarase/histidase_N.
IPR005921. HutH.
IPR008948. L-Aspartase-like.
IPR022313. Phe/His_NH3-lyase_AS.
[Graphical view]
PfamiPF00221. Lyase_aromatic. 1 hit.
[Graphical view]
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR01225. hutH. 1 hit.
PROSITEiPS00488. PAL_HISTIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHUTH_BRASO
AccessioniPrimary (citable) accession number: A4YNK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: September 7, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.