A4YNK6 (HUTI_BRASO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Imidazolonepropionase
EC=3.5.2.7

Alternative name(s):
Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	BRADO1603

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity. HAMAP MF_00372
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential HAMAP MF_00372.
Sequence similarities	Belongs to the HutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 404	404	Imidazolonepropionase HAMAP MF_00372		PRO_0000306442
Sites
	Metal binding	73	1	Zinc or iron By similarity
	Metal binding	75	1	Zinc or iron By similarity
	Metal binding	243	1	Zinc or iron By similarity
	Metal binding	318	1	Zinc or iron By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	95	1	Substrate By similarity
	Binding site	145	1	Substrate By similarity
	Binding site	178	1	Substrate By similarity
	Binding site	246	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4YNK6 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 641A95D82AC61D55	FASTA	404	42,831
	10 20 30 40 50 60 MAQRFDRIWQ NARLATLGEG RPGLGVIEHG VVASRAGRIA FAGPQSDFAA DADAPERIDC 70 80 90 100 110 120 EGRWITPGLI DCHTHLVYGG DRAHEFELRL AGASYEEIAR AGGGIVSTVA ATRAASEAEL 130 140 150 160 170 180 IASALPRLDA LLAEGVTTIE IKSGYGLETA TELRQLSAAR ALGEQRPVSV RTSFLGAHAL 190 200 210 220 230 240 PVEAGGDKER YIDLVCNEML PAVAQSGAAD AVDAFMETIA FSPEQTSRVF AAAKTLGLPV 250 260 270 280 290 300 KLHADQLSNL GGAALAAGFG ALSADHLEHT DEVGAVAMAR SGTVAVLLPG AYYFIRETQK 310 320 330 340 350 360 PPVELFRKHG VKLALATDCN PGSSPLTSLL LTMNMAATLF RMTVDECIAG VTREGARALG 370 380 390 400 LLADTGTLEP GKWCNLAIWD IGRPAELVYR LGFNPLHQRV WRGR

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ORS278.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU234118 Genomic DNA. Translation: CAL75482.1.
RefSeq	YP_001203719.1. NC_009445.1.
3D structure databases
ProteinModelPortal	A4YNK6.
SMR	A4YNK6. Positions 8-404.
ModBase	Search...
Protein-protein interaction databases
STRING	A4YNK6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5118395.
GenomeReviews	Gene locus BRADO1603 in contig CU234118_GR.
KEGG	bra:BRADO1603.
PATRIC	21219429. VBIBraSp122330_1539.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1228.
HOGENOM	HBG686142.
OMA	MNMACTL.
ProtClustDB	PRK09356.
Enzyme and pathway databases
BioCyc	BSP376:BRADO1603-MONOMER.
Family and domain databases
HAMAP	MF_00372. HutI. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
KO	K01468.
PANTHER	PTHR22642. PTHR22642. 1 hit.
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMs	TIGR01224. HutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_BRASO

Accession

Primary (citable) accession number: A4YNK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	May 29, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program