Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A4YMC5 (RUVC_BRASO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:BRADO1144
OrganismBradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]
Taxonomic identifier114615 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length175 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 175175Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_1000002724

Sites

Metal binding161Magnesium By similarity
Metal binding761Magnesium By similarity
Metal binding1481Magnesium By similarity
Metal binding1511Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
A4YMC5 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: A1F4F3EFDA59E467

FASTA17518,390
        10         20         30         40         50         60 
MKAQPIRAAI RIIGIDPGLR RTGWGVIESE GNRLIYVGCG SVEPPDDLPL ASRLLAIHEG 

        70         80         90        100        110        120 
LAKVLADFQP LEAAVEQTFV NKDGVATLKL GQARGIAMLA PAMFGITVAE YAPNQVKKTV 

       130        140        150        160        170 
VGAGHADKGQ IAVMLKILLP KAEPPSADAA DALAIAITHA HHRQGQALRM KVAGL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU234118 Genomic DNA. Translation: CAL75051.1.
RefSeqYP_001203288.1. NC_009445.1.

3D structure databases

ProteinModelPortalA4YMC5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING114615.BRADO1144.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL75051; CAL75051; BRADO1144.
GeneID5114959.
KEGGbra:BRADO1144.
PATRIC21218521. VBIBraSp122330_1089.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012181.
KOK01159.
OMAYTALQMK.
OrthoDBEOG6RG044.
ProtClustDBPRK00039.

Enzyme and pathway databases

BioCycBSP114615:GJN5-1089-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUVC_BRASO
AccessionPrimary (citable) accession number: A4YMC5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: April 16, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families