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A4YM47

- MASZ_BRASO

UniProt

A4YM47 - MASZ_BRASO

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Protein

Malate synthase G

Gene

glcB

Organism
Bradyrhizobium sp. (strain ORS278)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei117 – 1171Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei274 – 2741Acetyl-CoAUniRule annotation
Binding sitei311 – 3111Acetyl-CoAUniRule annotation
Active sitei338 – 3381Proton acceptorUniRule annotation
Binding sitei338 – 3381GlyoxylateUniRule annotation
Metal bindingi430 – 4301MagnesiumUniRule annotation
Binding sitei430 – 4301GlyoxylateUniRule annotation
Metal bindingi458 – 4581MagnesiumUniRule annotation
Binding sitei539 – 5391Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei629 – 6291Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBSP114615:GJN5-1011-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:BRADO1059
OrganismiBradyrhizobium sp. (strain ORS278)
Taxonomic identifieri114615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000001994: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 724724Malate synthase GPRO_1000056896Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei615 – 6151Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi114615.BRADO1059.

Structurei

3D structure databases

ProteinModelPortaliA4YM47.
SMRiA4YM47. Positions 9-718.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1252Acetyl-CoA bindingUniRule annotation
Regioni455 – 4584Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiSQFIENE.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

A4YM47-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTRIDAHGLK IAPVLFDFIT KEAAPKTGID PDAFWAGVAA ILRDLGSKNR
60 70 80 90 100
ELLKFRDTLQ DKIDAWHLAN KGKAFDLGAY TAFLKEIGYL LPEPATQKVE
110 120 130 140 150
TANVDDEIGK ICGPQLVVPL TNARYALNAA NARWGSLYDA FYGTDAIPHD
160 170 180 190 200
PSEGGKGYNK ARGDKVIAKA KAFLDAAAPL ATGSHTDVTA YSVIAGQLSA
210 220 230 240 250
KLKSGNATAL KNAGQFAGYQ GSAEAPSVIL LANNGLHVEV VIDRSHVIGK
260 270 280 290 300
DDPAGVSDMI LEAAVSTILD MEDSVAAVDA EDKVLVYRNT LGLMNGTLSA
310 320 330 340 350
DFEKGGKTLT RALNSDRSYT TPSGGTLALH GRSLLLIRNC GHHMFTDAVL
360 370 380 390 400
GADGEEIPEG ILDAAVTGLL AIHDLKGHSK VKNSRTGSIY IVKPKMHGPD
410 420 430 440 450
EVALTVELFA RVEKMLGLPE NTMKVGIMDE ERRTTVNLKA CIQQASKRIV
460 470 480 490 500
FINTGFLDRT GDEIHTSMEA GPMIRKNEMK AQPWIKAYED WNVDMGLIDG
510 520 530 540 550
LPGHAQIGKG MWAAPDKMAD MLAQKIGHPS AGATTAWVPS PTAATLHALH
560 570 580 590 600
YHQVNVKARQ DELTKGGPRA KLGDILTIPV SNSNWAPDDV RQEIDNNCQG
610 620 630 640 650
ILGYVVRWID QGVGCSKVPD IHDVGLMEDR ATLRISSQHL ANWLHHGVVS
660 670 680 690 700
KDQVMDSLKR MAVVVDKQNA GDPLYQPMAP SFDGVAFKAA CDLVFEGRTQ
710 720
PNGYTEYILT ARRREAKASE TAKA
Length:724
Mass (Da):78,074
Last modified:May 29, 2007 - v1
Checksum:iA5916C8C3EBD7D52
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU234118 Genomic DNA. Translation: CAL74973.1.
RefSeqiWP_011924220.1. NC_009445.1.
YP_001203210.1. NC_009445.1.

Genome annotation databases

EnsemblBacteriaiCAL74973; CAL74973; BRADO1059.
GeneIDi5114900.
KEGGibra:BRADO1059.
PATRICi21218351. VBIBraSp122330_1004.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU234118 Genomic DNA. Translation: CAL74973.1 .
RefSeqi WP_011924220.1. NC_009445.1.
YP_001203210.1. NC_009445.1.

3D structure databases

ProteinModelPortali A4YM47.
SMRi A4YM47. Positions 9-718.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 114615.BRADO1059.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL74973 ; CAL74973 ; BRADO1059 .
GeneIDi 5114900.
KEGGi bra:BRADO1059.
PATRICi 21218351. VBIBraSp122330_1004.

Phylogenomic databases

eggNOGi COG2225.
HOGENOMi HOG000220740.
KOi K01638.
OMAi SQFIENE.
OrthoDBi EOG6HJ286.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .
BioCyci BSP114615:GJN5-1011-MONOMER.

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ORS278.

Entry informationi

Entry nameiMASZ_BRASO
AccessioniPrimary (citable) accession number: A4YM47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: October 29, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3