A4YM32 (UREF_BRASO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urease accessory protein UreF

Gene names

Name:	ureF
Ordered Locus Names:	BRADO1044

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for maturation of urease via the functional incorporation of the urease nickel metallocenter By similarity. HAMAP MF_01385
Subunit structure	UreD, UreF and UreG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of UreC. The UreE protein probably delivers the nickel By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_01385.
Sequence similarities	Belongs to the UreF family.

Ontologies

Keywords
Biological process	Nickel insertion
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	Urease accessory protein UreF HAMAP MF_01385		PRO_0000344082

Sequences

	Sequence		Length	Mass (Da)
	A4YM32 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 726AA43FCA5D8A06	FASTA	240	25,431
	10 20 30 40 50 60 MTTNDAGRIP SAAGGAELAA LYRLLTWLSP AFPIGGFSYS SGLEWAVEAG DICDAAALRG 70 80 90 100 110 120 WLATMLTDGS GFCDAAFLVH AHRATELDDI KRLSEVAELA AAFVPSRERQ LETAAQGRAF 130 140 150 160 170 180 IEIARSAWSC AGLDEAVAQC DVVVYPVAVG MVGALHGVPL EPLLHGFLHA LVSNWISAGS 190 200 210 220 230 240 RLVPLGQTDS QRVLAALEPV VVATADRSLH ASLDDIGSAT FRADLASLRH ETQYTRLFRS

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ORS278.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU234118 Genomic DNA. Translation: CAL74958.1.
RefSeq	YP_001203195.1. NC_009445.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4YM32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5114822.
GenomeReviews	Gene locus BRADO1044 in contig CU234118_GR.
KEGG	bra:BRADO1044.
PATRIC	21218321. VBIBraSp122330_0989.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0830.
HOGENOM	HBG709295.
OMA	WLSPAFP.
ProtClustDB	CLSK818588.
Enzyme and pathway databases
BioCyc	BSP376:BRADO1044-MONOMER.
Family and domain databases
HAMAP	MF_01385. UreF. [Tree]
InterPro	IPR002639. UreF. [Graphical view]
KO	K03188.
Pfam	PF01730. UreF. 1 hit. [Graphical view]
PIRSF	PIRSF009467. Ureas_acces_UreF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UREF_BRASO

Accession

Primary (citable) accession number: A4YM32

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	May 29, 2007
Last modified:	December 14, 2011
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program