Reviewed, UniProtKB/Swiss-Prot A4YLX6 (GLPK_BRASO)

Names and origin

Protein names

Recommended name:
    Glycerol kinase
    EC=2.7.1.30
Alternative name(s):
    ATP:glycerol 3-phosphotransferase
    Glycerokinase
      Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	BRADO0987

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glycerol kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Glycerol kinase HAMAP MF_00186		PRO_1000020707
Regions
	Nucleotide binding	411 – 415	5	ATP By similarity
Sites
	Binding site	11	1	Substrate By similarity
	Binding site	15	1	ATP By similarity
	Binding site	81	1	Substrate By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	242	1	Substrate By similarity
	Binding site	264	1	ATP By similarity
	Binding site	307	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4YLX6-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: ECCAE54F6226125C	FASTA	500	54,185
	10 20 30 40 50 60 MSFVLAIDQG TTSSRAMVFR SDISIAAVAQ QEFPQHFPAS GWVEHEPEDI WTSTVMTCRD 70 80 90 100 110 120 ALEKAGLAAK DIAAIGITNQ RETTVVWDRA TGQAVHRAIV WQDRRTADIC AKLKAEGHEP 130 140 150 160 170 180 DVSARTGLII DPYFSGTKVA WILDHVPGAR ERAERGELLF GTVDCYLLWR LTGGRVHATD 190 200 210 220 230 240 ATNASRTLLF NIHTGQWDDT LLKLLRVPRS MLPEVKDSSA DFGTTTPDLF GGPIKVAGIA 250 260 270 280 290 300 GDQQAATIGQ ACFTPGMMKS TYGTGCFALL NTGATPVKSN NKLLTTIAYQ LNGIRTYALE 310 320 330 340 350 360 GSIFVAGSAV QWLRDGLGII KHAAETGPLA DKSDSMQSVY LVPAFVGLGA PYWNPRVRGA 370 380 390 400 410 420 LFGLTRNTGP AELAHATLES VCYQTYDLWA AMRADWPDAS AATIVLRVDG GMTASDWTMQ 430 440 450 460 470 480 RLADLLDAPV DRPMIQETTA LGAAYLAGLN AGVYPEPEKF ADNWRLEHRF RPAMSAATRQ 490 500 RKLAGWARAV KGVLASDEGE

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU234118 Genomic DNA. Translation: CAL74902.1.
RefSeq	YP_001203139.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4YLX6.
Genome annotation databases
GeneID	5117577.
GenomeReviews	Gene locus BRADO0987 in contig CU234118_GR.
KEGG	bra:BRADO0987.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KFADNWR.
Family and domain databases
HAMAP	MF_00186. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_BRASO

Accession

Primary (citable) accession number: A4YLX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)