A4YLC7 (ARGB_BRASO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylglutamate kinase
EC=2.7.2.8

Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferase
NAG kinase
Short name=AGK

Gene names

Name:	argB
Ordered Locus Names:	BRADO0779

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082_B
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00082_B.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetylglutamate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	Acetylglutamate kinase HAMAP MF_00082_B		PRO_1000010487
Regions
	Region	69 – 70	2	Substrate binding By similarity
Sites
	Binding site	91	1	Substrate By similarity
	Binding site	196	1	Substrate By similarity
	Site	34	1	Transition state stabilizer By similarity
	Site	256	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4YLC7 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 61C6D0F5C7A81720	FASTA	298	31,555
	10 20 30 40 50 60 MTDATTISPL DQARILSEAL PHMQEYDDET IVIKYGGHAM GDEDTAKAFA RDIVLLEQTA 70 80 90 100 110 120 INPVVVHGGG PQIATMLKRL GIVSEFAAGL RITDAATIEI VEMVLAGSIN KQLVGYINEA 130 140 150 160 170 180 GGKAVGLCGK DGNMVRAVKA TRTMVDPDSH IEKVVDLGFV GEPDKVDLTL LNQLIGYELI 190 200 210 220 230 240 PVLAPLATSK EGQTFNVNAD TFAGAVAGAL KAKRLLLLTD VPGVLDKSKK LIPEISIKDA 250 260 270 280 290 RKLIADGTIS GGMIPKVETC IYALEQGVQG VVILDGKVPH AVLLELFTNQ GTGTLIHK

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ORS278.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU234118 Genomic DNA. Translation: CAL74703.1.
RefSeq	YP_001202941.1. NC_009445.1.
3D structure databases
ProteinModelPortal	A4YLC7.
ModBase	Search...
Protein-protein interaction databases
STRING	A4YLC7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5115234.
GenomeReviews	Gene locus BRADO0779 in contig CU234118_GR.
KEGG	bra:BRADO0779.
PATRIC	21217819. VBIBraSp122330_0739.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0548.
HOGENOM	HBG497643.
OMA	GGNAMID.
ProtClustDB	PRK00942.
Enzyme and pathway databases
BioCyc	BSP376:BRADO0779-MONOMER.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00930.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00761. ArgB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_BRASO

Accession

Primary (citable) accession number: A4YLC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 29, 2007
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program