Reviewed, UniProtKB/Swiss-Prot A4YL97 (PYRE_BRASO)

Names and origin

Protein names

Recommended name:
    Orotate phosphoribosyltransferase
      Short name=OPRT
      Short name=OPRTase
    EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	BRADO0748

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity.
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 185	185	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_1000066212
Regions
	Region	128 – 136	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	98	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	99	1	5-phosphoribose 1-diphosphate By similarity
	Binding site	102	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	104	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	132	1	Orotate By similarity
	Binding site	160	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4YL97-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 3CF4736376895C96	FASTA	185	20,077
	10 20 30 40 50 60 MSKSASRARL LEIIRRRSFG RGEVTLASGR KSDFYFNLKP TMMDPEGATL LAELTYEALK 70 80 90 100 110 120 DDGYDYIGGL EMGAVPLAGA IAQISWIKGH PIAAFFVRKK PKEHGARLAI EGLTRDETLA 130 140 150 160 170 180 GKRIVVVEDV TTTGGSAMKA VETLREAGAE VSLVFTMVDR EEGAAEAFAA AGLSFRALYK AREFL

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU234118 Genomic DNA. Translation: CAL74673.1.
RefSeq	YP_001202911.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4YL97.
Genome annotation databases
GeneID	5115214.
GenomeReviews	Gene locus BRADO0748 in contig CU234118_GR.
KEGG	bra:BRADO0748.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EPKGHGT.
Family and domain databases
HAMAP	MF_01208. [Tree]
InterPro	IPR004467. Or_phspho_trans. IPR002375. Pr/py_Pribosyl_transf_CS. IPR000836. PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. pyrE. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_BRASO

Accession

Primary (citable) accession number: A4YL97

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)