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Protein

Phosphoenolpyruvate carboxykinase [ATP]

Gene

pckA

Organism
Bradyrhizobium sp. (strain ORS 278)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.UniRule annotation

Catalytic activityi

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61SubstrateUniRule annotation1
Binding sitei194SubstrateUniRule annotation1
Metal bindingi200ManganeseUniRule annotation1
Binding sitei200ATPUniRule annotation1
Binding sitei200SubstrateUniRule annotation1
Metal bindingi219Manganese; via tele nitrogenUniRule annotation1
Binding sitei219ATPUniRule annotation1
Metal bindingi256ManganeseUniRule annotation1
Binding sitei284ATPUniRule annotation1
Binding sitei322ATPUniRule annotation1
Binding sitei322SubstrateUniRule annotation1
Binding sitei448ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi235 – 243ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [ATP]UniRule annotation (EC:4.1.1.49UniRule annotation)
Short name:
PCKUniRule annotation
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckAUniRule annotation
Ordered Locus Names:BRADO0741
OrganismiBradyrhizobium sp. (strain ORS 278)
Taxonomic identifieri114615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000001994 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000263181 – 537Phosphoenolpyruvate carboxykinase [ATP]Add BLAST537

Proteomic databases

PRIDEiA4YL90.

Interactioni

Protein-protein interaction databases

STRINGi114615.BRADO0741.

Structurei

3D structure databases

ProteinModelPortaliA4YL90.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiPOG091H03CD.

Family and domain databases

CDDicd00484. PEPCK_ATP. 1 hit.
Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP. 1 hit.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4YL90-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQETGIRNGA FGADKFGLKN LKQLHWNLGA PQLYQYSLAA GEATLSADGA
60 70 80 90 100
LCADTGEFTG RSPKDKFTVR DASTEKMWWA GNQSITPEQF EALYQDFIKH
110 120 130 140 150
AEGKTLFAQD LYGGADPTFR IKTRVFTELA WHSLFIRTLL IRPETIELDT
160 170 180 190 200
FVPELTIIDM PSFRADPMRH GVRSQNVVAI DFARKIVLIG GSYYAGEMKK
210 220 230 240 250
SVFTTLNYYL PARGVMPMHC SANVGPKGDT AIFFGLSGTG KTTLSADPNR
260 270 280 290 300
TLIGDDEHGW GPSGVFNFEG GCYAKCIKLS QEAEPQIFAA SNRFGAVLEN
310 320 330 340 350
VVLDEDSRVP DFDDGSKTEN TRSAYPLDYI PNASRTGRAP HPKNVVMLAA
360 370 380 390 400
DAFGVLPPIA KLSPAQAMYH FLSGYTAKVA GTERGLGSEP QPEFSTCFGS
410 420 430 440 450
PFLPLDPSVY GNMLRQLIAD HNVDCWLVNT GWTGGKYGTG SRMPIKVTRA
460 470 480 490 500
LLTAALDGSL RNVEFRTDKY FGFAVPTALP GVPSEILDPV NTWKDKVEFD
510 520 530
KTARALVGMF QKNFAKFEAQ VDADVRAAAP DVKLAAE
Length:537
Mass (Da):58,743
Last modified:May 29, 2007 - v1
Checksum:i63D1027DD4BDD5E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU234118 Genomic DNA. Translation: CAL74666.1.
RefSeqiWP_011923925.1. NC_009445.1.

Genome annotation databases

EnsemblBacteriaiCAL74666; CAL74666; BRADO0741.
KEGGibra:BRADO0741.
PATRICi21217745. VBIBraSp122330_0702.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU234118 Genomic DNA. Translation: CAL74666.1.
RefSeqiWP_011923925.1. NC_009445.1.

3D structure databases

ProteinModelPortaliA4YL90.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi114615.BRADO0741.

Proteomic databases

PRIDEiA4YL90.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL74666; CAL74666; BRADO0741.
KEGGibra:BRADO0741.
PATRICi21217745. VBIBraSp122330_0702.

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiPOG091H03CD.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

CDDicd00484. PEPCK_ATP. 1 hit.
Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP. 1 hit.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCKA_BRASO
AccessioniPrimary (citable) accession number: A4YL90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: November 2, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.