Reviewed, UniProtKB/Swiss-Prot A4YKP3 (GLND_BRASO)

Names and origin

Protein names

Recommended name:
    [Protein-PII] uridylyltransferase
      Short name=PII uridylyl-transferase
    EC=2.7.7.59
Alternative name(s):
    Uridylyl-removing enzyme
    UTase

Gene names

Name:	glnD
Ordered Locus Names:	BRADO0530

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	931 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Modifies, by uridylylation or deuridylylation the PII (glnB) regulatory protein By similarity.
Catalytic activity	UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII]. HAMAP MF_00277
Sequence similarities	Belongs to the glnD family.

Ontologies

Keywords
Biological process	Nitrogen fixation
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nitrogen fixation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	[protein-PII] uridylyltransferase activity Inferred from electronic annotation. Source: HAMAP amino acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 931	931	[Protein-PII] uridylyltransferase HAMAP MF_00277		PRO_1000022328

Sequences

	Sequence		Length	Mass (Da)
	A4YKP3-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: EF1CED6FAB25A7D4	FASTA	931	104,696
	10 20 30 40 50 60 MDSVATDSKA FTSADFDSAG IAAAVDALAK QHSGREDMFR AAVVQFLKAE LVKARAAAQA 70 80 90 100 110 120 QLLQDRHGRH CAERLCVVQD EIIRILYAAA TEHLYRSDVP SGAERMAVVA TGGYGRGLMA 130 140 150 160 170 180 PESDIDLLFI LPYKQTAWGE QVAEAILYSL WDMGLKVGHA TRSVDESIRQ ARGDMTIRTA 190 200 210 220 230 240 ILETRYLAGD KPLYDELVQR FDTEVVQGTA AEFVAAKLAE REERHRRGGQ SRYLVEPNVK 250 260 270 280 290 300 DGKGGLRDLH TLFWIAKYVY RVRETAELVE RGVFDAHEYR TFRRCSDFLW SVRCNLHFVS 310 320 330 340 350 360 GRPEERLSFD LQREIAVRLG YTSHPGMQDV ERFMKHYFLV AKQVGNLTAI LCAKLEDQQA 370 380 390 400 410 420 KAAPVLSRMI SRLTTGSTWR RVPESDDFIV DNNRINLAAP DVFKHDPVNL IRIFRLAQKN 430 440 450 460 470 480 NLAFHPDAMR AVTRSLNMIN AELRENPEAN RLFMEILTSN DAETVLRRMN ETGVLGHFIS 490 500 510 520 530 540 AFGRIVSMMQ FNMYHHYTVD EHLIRCIGFL QEIERGGHDE FVLASDLMRK IRPEHRAVIY 550 560 570 580 590 600 ITVLLHDVAK GRPEDHSVAG AKIARRLCPR LGFNNADTEL VAWLIEEHLT MSTVAQSRDL 610 620 630 640 650 660 SDRRTIEKFA AVVQSVEQMK LLTILTTADI RGVGPGVWNG WKAQLLRTLY YETEPVLTGG 670 680 690 700 710 720 FSEVNRAKRI TAAQAEFRNA FTDWPEDELN TYIGRHYPAY WLKVELPRKI RHARFVRSSE 730 740 750 760 770 780 DAGHKLAINV GFDPARGVTE LTIFAMDHPW LLSIIAGACA SAGANIVDAQ IYTTTDGRAL 790 800 810 820 830 840 DTIAISREYE RDEDEGRRAT RIGETIEQVL EGKLRLPDAV ARRTTRGKQH KAFSVEPEVS 850 860 870 880 890 900 INNQWSELYT VIEVSGLDRP GLLYELTTAI SKLNLNIASA HVATFGERAR DVFYVTDLLG 910 920 930 AQINAPTRQA AIKSALLHLL ASEDAAAQPA A

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU234118 Genomic DNA. Translation: CAL74469.1.
RefSeq	YP_001202712.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4YKP3.
Genome annotation databases
GeneID	5115303.
GenomeReviews	Gene locus BRADO0530 in contig CU234118_GR.
KEGG	bra:BRADO0530.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MQHDLFH.
Family and domain databases
HAMAP	MF_00277. [Tree]
InterPro	IPR002912. ACT_bd. IPR010043. GlnD_Uridyltrans. IPR003607. Met-dep_phosphohydro_HD. IPR006674. Met-dep_phosphohydro_HD_sub. IPR002934. Nucleotidyltransferase. IPR013546. PII_UdlTrfase/GS_AdlTrfase. [Graphical view]
PANTHER	PTHR13734:SF1. GlnD_Uridyltrans. 1 hit.
Pfam	PF01842. ACT. 2 hits. PF08335. GlnD_UR_UTase. 1 hit. PF01966. HD. 1 hit. PF01909. NTP_transf_2. 1 hit. [Graphical view]
PIRSF	PIRSF006288. PII_uridyltransf. 1 hit.
SMART	SM00471. HDc. 1 hit. [Graphical view]
TIGRFAMs	TIGR01693. UTase_glnD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLND_BRASO

Accession

Primary (citable) accession number: A4YKP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)