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A4YKP3 (GLND_BRASO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme

Short name=UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein

Including the following 2 domains:

  1. [Protein-PII] uridylyltransferase
    Short name=PII uridylyltransferase
    Short name=UTase
    EC=2.7.7.59
  2. [Protein-PII]-UMP uridylyl-removing enzyme
    Short name=UR
    EC=3.1.4.-
Gene names
Name:glnD
Ordered Locus Names:BRADO0530
OrganismBradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]
Taxonomic identifier114615 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length931 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism By similarity. HAMAP-Rule MF_00277

Catalytic activity

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII]. HAMAP-Rule MF_00277

Uridylyl-[protein-PII] + H2O = UMP + [protein-PII]. HAMAP-Rule MF_00277

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00277

Enzyme regulation

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity. HAMAP-Rule MF_00277

Domain

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity. HAMAP-Rule MF_00277

Sequence similarities

Belongs to the GlnD family.

Contains 2 ACT domains.

Contains 1 HD domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 931931Bifunctional uridylyltransferase/uridylyl-removing enzyme HAMAP-Rule MF_00277
PRO_1000022328

Regions

Domain502 – 59998HD
Domain740 – 82283ACT 1
Domain851 – 93181ACT 2
Region1 – 383383Uridylyltransferase HAMAP-Rule MF_00277
Region384 – 739356Uridylyl-removing HAMAP-Rule MF_00277

Sequences

Sequence LengthMass (Da)Tools
A4YKP3 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: EF1CED6FAB25A7D4

FASTA931104,696
        10         20         30         40         50         60 
MDSVATDSKA FTSADFDSAG IAAAVDALAK QHSGREDMFR AAVVQFLKAE LVKARAAAQA 

        70         80         90        100        110        120 
QLLQDRHGRH CAERLCVVQD EIIRILYAAA TEHLYRSDVP SGAERMAVVA TGGYGRGLMA 

       130        140        150        160        170        180 
PESDIDLLFI LPYKQTAWGE QVAEAILYSL WDMGLKVGHA TRSVDESIRQ ARGDMTIRTA 

       190        200        210        220        230        240 
ILETRYLAGD KPLYDELVQR FDTEVVQGTA AEFVAAKLAE REERHRRGGQ SRYLVEPNVK 

       250        260        270        280        290        300 
DGKGGLRDLH TLFWIAKYVY RVRETAELVE RGVFDAHEYR TFRRCSDFLW SVRCNLHFVS 

       310        320        330        340        350        360 
GRPEERLSFD LQREIAVRLG YTSHPGMQDV ERFMKHYFLV AKQVGNLTAI LCAKLEDQQA 

       370        380        390        400        410        420 
KAAPVLSRMI SRLTTGSTWR RVPESDDFIV DNNRINLAAP DVFKHDPVNL IRIFRLAQKN 

       430        440        450        460        470        480 
NLAFHPDAMR AVTRSLNMIN AELRENPEAN RLFMEILTSN DAETVLRRMN ETGVLGHFIS 

       490        500        510        520        530        540 
AFGRIVSMMQ FNMYHHYTVD EHLIRCIGFL QEIERGGHDE FVLASDLMRK IRPEHRAVIY 

       550        560        570        580        590        600 
ITVLLHDVAK GRPEDHSVAG AKIARRLCPR LGFNNADTEL VAWLIEEHLT MSTVAQSRDL 

       610        620        630        640        650        660 
SDRRTIEKFA AVVQSVEQMK LLTILTTADI RGVGPGVWNG WKAQLLRTLY YETEPVLTGG 

       670        680        690        700        710        720 
FSEVNRAKRI TAAQAEFRNA FTDWPEDELN TYIGRHYPAY WLKVELPRKI RHARFVRSSE 

       730        740        750        760        770        780 
DAGHKLAINV GFDPARGVTE LTIFAMDHPW LLSIIAGACA SAGANIVDAQ IYTTTDGRAL 

       790        800        810        820        830        840 
DTIAISREYE RDEDEGRRAT RIGETIEQVL EGKLRLPDAV ARRTTRGKQH KAFSVEPEVS 

       850        860        870        880        890        900 
INNQWSELYT VIEVSGLDRP GLLYELTTAI SKLNLNIASA HVATFGERAR DVFYVTDLLG 

       910        920        930 
AQINAPTRQA AIKSALLHLL ASEDAAAQPA A 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU234118 Genomic DNA. Translation: CAL74469.1.
RefSeqYP_001202712.1. NC_009445.1.

3D structure databases

ProteinModelPortalA4YKP3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING114615.BRADO0530.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL74469; CAL74469; BRADO0530.
GeneID5115303.
KEGGbra:BRADO0530.
PATRIC21217330. VBIBraSp122330_0496.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2844.
HOGENOMHOG000261779.
KOK00990.
OMASPKVWNA.
OrthoDBEOG6CCH44.
ProtClustDBPRK05092.

Enzyme and pathway databases

BioCycBSP114615:GJN5-503-MONOMER.

Family and domain databases

Gene3D1.10.3210.10. 1 hit.
HAMAPMF_00277. PII_uridylyl_transf.
InterProIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PANTHERPTHR13734:SF1. PTHR13734:SF1. 1 hit.
PfamPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFPIRSF006288. PII_uridyltransf. 1 hit.
SMARTSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsTIGR01693. UTase_glnD. 1 hit.
PROSITEPS51671. ACT. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLND_BRASO
AccessionPrimary (citable) accession number: A4YKP3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: February 19, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families