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A4YKP3

- GLND_BRASO

UniProt

A4YKP3 - GLND_BRASO

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Protein
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Gene
glnD, BRADO0530
Organism
Bradyrhizobium sp. (strain ORS278)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen fixation Source: UniProtKB-HAMAP
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciBSP114615:GJN5-503-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:BRADO0530
OrganismiBradyrhizobium sp. (strain ORS278)
Taxonomic identifieri114615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000001994: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 931931Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
PRO_1000022328Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi114615.BRADO0530.

Structurei

3D structure databases

ProteinModelPortaliA4YKP3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini502 – 59998HD
Add
BLAST
Domaini740 – 82283ACT 1
Add
BLAST
Domaini851 – 93181ACT 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 383383UridylyltransferaseUniRule annotation
Add
BLAST
Regioni384 – 739356Uridylyl-removingUniRule annotation
Add
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4YKP3-1 [UniParc]FASTAAdd to Basket

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MDSVATDSKA FTSADFDSAG IAAAVDALAK QHSGREDMFR AAVVQFLKAE    50
LVKARAAAQA QLLQDRHGRH CAERLCVVQD EIIRILYAAA TEHLYRSDVP 100
SGAERMAVVA TGGYGRGLMA PESDIDLLFI LPYKQTAWGE QVAEAILYSL 150
WDMGLKVGHA TRSVDESIRQ ARGDMTIRTA ILETRYLAGD KPLYDELVQR 200
FDTEVVQGTA AEFVAAKLAE REERHRRGGQ SRYLVEPNVK DGKGGLRDLH 250
TLFWIAKYVY RVRETAELVE RGVFDAHEYR TFRRCSDFLW SVRCNLHFVS 300
GRPEERLSFD LQREIAVRLG YTSHPGMQDV ERFMKHYFLV AKQVGNLTAI 350
LCAKLEDQQA KAAPVLSRMI SRLTTGSTWR RVPESDDFIV DNNRINLAAP 400
DVFKHDPVNL IRIFRLAQKN NLAFHPDAMR AVTRSLNMIN AELRENPEAN 450
RLFMEILTSN DAETVLRRMN ETGVLGHFIS AFGRIVSMMQ FNMYHHYTVD 500
EHLIRCIGFL QEIERGGHDE FVLASDLMRK IRPEHRAVIY ITVLLHDVAK 550
GRPEDHSVAG AKIARRLCPR LGFNNADTEL VAWLIEEHLT MSTVAQSRDL 600
SDRRTIEKFA AVVQSVEQMK LLTILTTADI RGVGPGVWNG WKAQLLRTLY 650
YETEPVLTGG FSEVNRAKRI TAAQAEFRNA FTDWPEDELN TYIGRHYPAY 700
WLKVELPRKI RHARFVRSSE DAGHKLAINV GFDPARGVTE LTIFAMDHPW 750
LLSIIAGACA SAGANIVDAQ IYTTTDGRAL DTIAISREYE RDEDEGRRAT 800
RIGETIEQVL EGKLRLPDAV ARRTTRGKQH KAFSVEPEVS INNQWSELYT 850
VIEVSGLDRP GLLYELTTAI SKLNLNIASA HVATFGERAR DVFYVTDLLG 900
AQINAPTRQA AIKSALLHLL ASEDAAAQPA A 931
Length:931
Mass (Da):104,696
Last modified:May 29, 2007 - v1
Checksum:iEF1CED6FAB25A7D4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU234118 Genomic DNA. Translation: CAL74469.1.
RefSeqiWP_011923740.1. NC_009445.1.
YP_001202712.1. NC_009445.1.

Genome annotation databases

EnsemblBacteriaiCAL74469; CAL74469; BRADO0530.
GeneIDi5115303.
KEGGibra:BRADO0530.
PATRICi21217330. VBIBraSp122330_0496.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU234118 Genomic DNA. Translation: CAL74469.1 .
RefSeqi WP_011923740.1. NC_009445.1.
YP_001202712.1. NC_009445.1.

3D structure databases

ProteinModelPortali A4YKP3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 114615.BRADO0530.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL74469 ; CAL74469 ; BRADO0530 .
GeneIDi 5115303.
KEGGi bra:BRADO0530.
PATRICi 21217330. VBIBraSp122330_0496.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261779.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci BSP114615:GJN5-503-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ORS278.

Entry informationi

Entry nameiGLND_BRASO
AccessioniPrimary (citable) accession number: A4YKP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: September 3, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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