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A4YKF1 (PROA_BRASO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41
Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase
Gene names
Name:proA
Ordered Locus Names:BRADO0430
OrganismBradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]
Taxonomic identifier114615 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length429 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412

Catalytic activity

L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412

Subcellular location

Cytoplasm By similarity HAMAP MF_00412.

Sequence similarities

Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Proline biosynthesis
   Cellular componentCytoplasm
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processproline biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionNADP binding

Inferred from electronic annotation. Source: InterPro

glutamate-5-semialdehyde dehydrogenase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 429429Gamma-glutamyl phosphate reductase HAMAP MF_00412
PRO_1000049938

Sequences

Sequence LengthMass (Da)Tools
A4YKF1 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: A500F9D664824371

FASTA42945,095
        10         20         30         40         50         60 
MTAPLKAIDG SADLPQLMND LAKRARAAAR VLALAPAEQK NRALEAMERR IRARADVIIA 

        70         80         90        100        110        120 
ANAEDVAEAK AVGVTPSFVD RLTLTPARVA AMADGIAVVR GVADPVGVVT ESWQRPNGMT 

       130        140        150        160        170        180 
IERVRVPLGV IGVIFESRPN VAADAGVLCL KSGNAVILRG GSDSFRSCRA IHECLVEGLR 

       190        200        210        220        230        240 
EAGLPDTAIT LVPTRDRAAV GLLLSGLNGG VDVIVPRGGK SLVARVEAEA RVPVFAHLEG 

       250        260        270        280        290        300 
VNHVYVDGAA DLDMAKSIVL NAKMRRTGVC GAAETLLIDR ASQDKIKPLV DMLIGAGCEV 

       310        320        330        340        350        360 
RGDEAVRAAD ARVTPANNED WDTEYLDAII AAKVVDGVDG AIAHIHAHGS HHTDAIVTQD 

       370        380        390        400        410        420 
NKVADKFLNE VDSAIVLHNA STQFADGGEF GFGAEIGIAT GKFHARGPVG VEQLTSFKYR 


IHGTGQTRP

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CU234118 Genomic DNA. Translation: CAL74377.1.
RefSeqYP_001202623.1. NC_009445.1.

3D structure databases

ProteinModelPortalA4YKF1.
ModBaseSearch...

Protein-protein interaction databases

STRINGA4YKF1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5116228.
GenomeReviewsGene locus BRADO0430 in contig CU234118_GR.
KEGGbra:BRADO0430.
PATRIC21217166. VBIBraSp122330_0416.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0014.
HOGENOMHBG318080.
OMAYGICGAM.
ProtClustDBPRK00197.

Enzyme and pathway databases

BioCycBSP376:BRADO0430-MONOMER.

Family and domain databases

HAMAPMF_00412. ProA.
[Tree]
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR000965. G-glutamylP_reductase.
IPR020593. G-glutamylP_reductase_CS.
IPR012134. Glu-5-SA_DH.
[Graphical view]
Gene3DG3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit.
G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KOK00147.
PANTHERPTHR11063:SF1. GSA_DH. 1 hit.
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFPIRSF000151. GPR. 1 hit.
SUPFAMSSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMsTIGR00407. ProA. 1 hit.
PROSITEPS01223. PROA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePROA_BRASO
AccessionPrimary (citable) accession number: A4YKF1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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