Reviewed, UniProtKB/Swiss-Prot A4YK73 (ACSA_BRASO)

Names and origin

Protein names

Recommended name:
    Acetyl-coenzyme A synthetase
    EC=6.2.1.1
Alternative name(s):
    Acetate--CoA ligase
    Acyl-activating enzyme

Gene names

Name:	acsA
Ordered Locus Names:	BRADO0344

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	650 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 650	650	Acetyl-coenzyme A synthetase HAMAP MF_01123		PRO_1000065273
Sites
	Active site	514	1	By similarity
Amino acid modifications
	Modified residue	606	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4YK73-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: AD5B4BD504BBCC0F	FASTA	650	72,335
	10 20 30 40 50 60 MSEKIYDVPA EWAKRAFVDD AKYQEMYASS IRDPNGFWAE QAKRVDWIHA PTKIENVSYA 70 80 90 100 110 120 PGNISIKWFE DGVLNAAYNC IDRHLATRAD QTAIIWEGDD PADSKHITYR QLHDEVCKMA 130 140 150 160 170 180 NILRNRNVKK GDRVTIYLPM IPEAAYAMLA CARIGAIHSV VFAGFSPDSL AQRIKDCDSK 190 200 210 220 230 240 VVITADEGLR GGRKVPLKAN VDAALNKVDN VDWVVVVKRT GGKIEMNPTR DLWYHEAAEM 250 260 270 280 290 300 VTTECPVEHM NAEDALFILY TSGSTGQPKG VLHTTGGYLV YASMTHQYVF DYHEGDVYWC 310 320 330 340 350 360 TADVGWVTGH SYILYGPLAN GAVTLMFEGV PNYPDNSRFW NVIDKHKVNI FYTAPTAIRA 370 380 390 400 410 420 LMQGGDGPVT KTSRESLRLL GSVGEPINPE AWEWYHRVVG DNRCPIVDTW WQTETGGILI 430 440 450 460 470 480 TPLPGATRLK PGSATRPFFG VVPEIVDADG NTLEGATEGN LCLTRSWPGQ MRTVYGDHAR 490 500 510 520 530 540 FEMTYFSTYK GKYFTGDGCR RDADGYYWIT GRVDDVINVS GHRMGTAEVE SALVAHPKVS 550 560 570 580 590 600 EAAVVGYPHD IKGQGIYAYV TLMAGETPSE ELRKELVGWV RKEIGPIASP DQIQFSQGLP 610 620 630 640 650 KTRSGKIMRR ILRKIAEDEP GALGDTSTLA DPAVVDDLVQ HRQNRKEKQA

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU234118 Genomic DNA. Translation: CAL74299.1.
RefSeq	YP_001202545.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4YK73.
Genome annotation databases
GeneID	5116186.
GenomeReviews	Gene locus BRADO0344 in contig CU234118_GR.
KEGG	bra:BRADO0344.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	ETASEHC.
Family and domain databases
HAMAP	MF_01123. [Tree]
InterPro	IPR011904. Ac_CoA_lig_AcsA. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_BRASO

Accession

Primary (citable) accession number: A4YK73

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)