A4YK54 (PNCB_BRASO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nicotinate phosphoribosyltransferase
Short name=NAPRTase
EC=2.4.2.11

Gene names

Name:	pncB
Ordered Locus Names:	BRADO0323

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Beta-nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_00570
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1. HAMAP-Rule MF_00570
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00570.
Sequence similarities	Belongs to the NAPRTase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	NAD biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway nicotinate nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	nicotinate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC nicotinate-nucleotide diphosphorylase (carboxylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	Nicotinate phosphoribosyltransferase HAMAP-Rule MF_00570		PRO_1000146834

Sequences

	Sequence		Length	Mass (Da)
	A4YK54 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 23B98C80298AFC4D	FASTA	434	49,506
	10 20 30 40 50 60 MTVTDIASRT YNHGWRLDPI VRSLLDTDFY KLLMLQMIRE FYPDQKVTFS VINRTKRVRL 70 80 90 100 110 120 GDVIDEGELR AQLDHARTIR FSKKELIWLA GNTFYGKTHM FSPDFLAWLA NFRLPEYELH 130 140 150 160 170 180 KADGQYELHF HGLWTHTTMW EIPALAIVNE LRSRQAMKGQ GRFALDVLFA RAKAKLWAKV 190 200 210 220 230 240 ERLRKLEGLR LSDFGTRRRH GFLWQRWCVE AVKEGLGSSF TGTSNVLLAM DNDLEAIGTN 250 260 270 280 290 300 AHELPMVAAA LAKDDDELRF APYRILDQWR QTYAGNLLIA LPDAFGTKAF LRDAPDWVAD 310 320 330 340 350 360 WTGFRPDSAP PIQAGEEIIA WWKSKGRDPR QKLLVFSDAM DVESIEQIHQ RFSDQVRLSF 370 380 390 400 410 420 GWGTNLTNDF VGCSPDGSVD LDPISLVCKV TSVDGRPAVK LSDNPEKATG DPAEIARYLR 430 VFGDAGRVRT PVFV

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ORS278.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU234118 Genomic DNA. Translation: CAL74280.1.
RefSeq	YP_001202526.1. NC_009445.1.
3D structure databases
ProteinModelPortal	A4YK54.
ModBase	Search...
Protein-protein interaction databases
STRING	114615.BRADO0323.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAL74280; CAL74280; BRADO0323.
GeneID	5116171.
KEGG	bra:BRADO0323.
PATRIC	21216962. VBIBraSp122330_0314.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1488.
HOGENOM	HOG000284929.
KO	K00763.
OMA	SFGWGTN.
ProtClustDB	PRK05321.
Enzyme and pathway databases
BioCyc	BSP114615:GJN5-312-MONOMER.
UniPathway	UPA00253; UER00457.
Family and domain databases
HAMAP	MF_00570. NAPRTase.
InterPro	IPR006406. Nic_PRibTrfase. IPR015977. Nic_PRibTrfase-like. IPR007229. Nic_PRibTrfase-rel. IPR002638. Quinolinate_PRibosylTrfase_C. [Graphical view]
PANTHER	PTHR11098:SF1. PTHR11098:SF1. 1 hit.
Pfam	PF04095. NAPRTase. 1 hit. [Graphical view]
PIRSF	PIRSF000484. NAPRT. 1 hit.
SUPFAM	SSF51690. Q_phspho_trans. 1 hit.
TIGRFAMs	TIGR01514. NAPRTase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PNCB_BRASO

Accession

Primary (citable) accession number: A4YK54

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 29, 2007
Last modified:	May 29, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program