Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional protein FolD

Gene

folD

Organism
Bradyrhizobium sp. (strain ORS 278)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.UniRule annotation
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.UniRule annotation

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei191NADPUniRule annotation1
Binding sitei232NADP; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi166 – 168NADPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Oxidoreductase
Biological processAmino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciBSP114615:G1GI7-270-MONOMER
UniPathwayiUPA00193

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolDUniRule annotation
Including the following 2 domains:
Methylenetetrahydrofolate dehydrogenaseUniRule annotation (EC:1.5.1.5UniRule annotation)
Methenyltetrahydrofolate cyclohydrolaseUniRule annotation (EC:3.5.4.9UniRule annotation)
Gene namesi
Name:folDUniRule annotation
Ordered Locus Names:BRADO0277
OrganismiBradyrhizobium sp. (strain ORS 278)
Taxonomic identifieri114615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000001994 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003057991 – 294Bifunctional protein FolDAdd BLAST294

Proteomic databases

PRIDEiA4YK09

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi114615.BRADO0277

Structurei

3D structure databases

ProteinModelPortaliA4YK09
SMRiA4YK09
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CN0 Bacteria
COG0190 LUCA
HOGENOMiHOG000218242
KOiK01491
OMAiAGKLCGD
OrthoDBiPOG091H0041

Family and domain databases

HAMAPiMF_01576 THF_DHG_CYH, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR000672 THF_DH/CycHdrlase
IPR020630 THF_DH/CycHdrlase_cat_dom
IPR020867 THF_DH/CycHdrlase_CS
IPR020631 THF_DH/CycHdrlase_NAD-bd_dom
PANTHERiPTHR10025 PTHR10025, 1 hit
PfamiView protein in Pfam
PF00763 THF_DHG_CYH, 1 hit
PF02882 THF_DHG_CYH_C, 1 hit
PRINTSiPR00085 THFDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00766 THF_DHG_CYH_1, 1 hit

Sequencei

Sequence statusi: Complete.

A4YK09-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTARIIDGKI IAADLRAQVA REVERVKHDL GLTPGLAVVL VGNDPASEVY
60 70 80 90 100
VRSKHTQTQA AGMASFEHKL PADVAQAELL ALISKLNQDP SVHGILVQLP
110 120 130 140 150
LPKGLDTEAV INAIDPAKDV DGLHPHNAGR LAGGQPALAP CTPLGCIILS
160 170 180 190 200
KTVHASLEGM NAIVIGRSNL VGRPLVQLLL NENATVTIAH SRSRDLPALT
210 220 230 240 250
ARADLVYAAV GRPEMVKRDW IKPGATVIDV GINRIPTPEG KTRLVGDVAY
260 270 280 290
AEVAEVAGAI TPVPGGVGQM TVACLLVNTL RAACAIAGLP KPSV
Length:294
Mass (Da):30,626
Last modified:May 29, 2007 - v1
Checksum:i15C280F20FEC4BBE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU234118 Genomic DNA Translation: CAL74235.1
RefSeqiWP_011923521.1, NC_009445.1

Genome annotation databases

EnsemblBacteriaiCAL74235; CAL74235; BRADO0277
KEGGibra:BRADO0277

Similar proteinsi

Entry informationi

Entry nameiFOLD_BRASO
AccessioniPrimary (citable) accession number: A4YK09
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: May 29, 2007
Last modified: May 23, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health