A4YJT3 (RSMG_BRASO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase G
EC=2.1.1.170

Alternative name(s):
16S rRNA 7-methylguanosine methyltransferase
Short name=16S rRNA m7G methyltransferase

Gene names

Name:	rsmG
Ordered Locus Names:	BRADO0194

Organism

Bradyrhizobium sp. (strain ORS278) [Complete proteome] [HAMAP]

Taxonomic identifier

114615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA By similarity. HAMAP MF_00074
Catalytic activity	S-adenosyl-L-methionine + guanosine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanosine(527) in 16S rRNA. HAMAP MF_00074
Subcellular location	Cytoplasm Potential HAMAP MF_00074.
Sequence similarities	Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	Ribosomal RNA small subunit methyltransferase G HAMAP MF_00074		PRO_0000335314

Sequences

	Sequence		Length	Mass (Da)
	A4YJT3 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 03D8261F4660E0F0	FASTA	222	24,090
	10 20 30 40 50 60 MKPTSSAAAR NELASDKAAA LALTPVSRET ERRLDRYIAL LLEWQAKTNL VAPSTLPHLW 70 80 90 100 110 120 TRHISDSLQL LDLAPEASFW VDLGSGGGFP GVVLACALAE RPGAKVHLVE RIAKKAAFLR 130 140 150 160 170 180 EAIRVTGSPG TVHLSEIGDI VEKWSGRVDC VTARALAPLH QLVGFAEPLV KKGAKALFLK 190 200 210 220 GQDVDAELTE STKYWKIEPK LHSSRTGGQG WIVAIDCIER RS

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ORS278.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU234118 Genomic DNA. Translation: CAL74159.1.
RefSeq	YP_001202409.1. NC_009445.1.
3D structure databases
ProteinModelPortal	A4YJT3.
ModBase	Search...
Protein-protein interaction databases
STRING	A4YJT3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5115700.
GenomeReviews	Gene locus BRADO0194 in contig CU234118_GR.
KEGG	bra:BRADO0194.
PATRIC	21216712. VBIBraSp122330_0192.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0357.
HOGENOM	HBG686577.
OMA	GGQGWIV.
ProtClustDB	PRK00107.
Enzyme and pathway databases
BioCyc	BSP376:BRADO0194-MONOMER.
Family and domain databases
HAMAP	MF_00074. 16SrRNA_methyltr_G. [Tree]
InterPro	IPR003682. rRNA_ssu_MeTfrase_G. [Graphical view]
KO	K03501.
Pfam	PF02527. GidB. 1 hit. [Graphical view]
PIRSF	PIRSF003078. GidB. 1 hit.
TIGRFAMs	TIGR00138. GidB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMG_BRASO

Accession

Primary (citable) accession number: A4YJT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 29, 2007
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program