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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Bradyrhizobium sp. (strain ORS278)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei633 – 6331ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSP114615:GJN5-183-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:BRADO0189
OrganismiBradyrhizobium sp. (strain ORS278)
Taxonomic identifieri114615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000001994 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 874874Leucine--tRNA ligasePRO_1000009301Add
BLAST

Proteomic databases

PRIDEiA4YJS8.

Interactioni

Protein-protein interaction databases

STRINGi114615.BRADO0189.

Structurei

3D structure databases

ProteinModelPortaliA4YJS8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionAdd
BLAST
Motifi630 – 6345"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4YJS8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSERYNARE AEPRWQRQWD EKAIFATKND DPRPKYYVLE MFPYPSGRIH
60 70 80 90 100
IGHVRNYTLG DVLARFMRAK GFNVLHPMGW DAFGLPAENA AIERKVAPKA
110 120 130 140 150
WTYDNIAAMK KQLRSIGLSL DWAREFATCD PSYYKHQQKM FLDFMQAGLV
160 170 180 190 200
EREQRKVNWD PVDMTVLANE QVIDGRGWRS GAVVEQREMN QWVFKITKYS
210 220 230 240 250
QELLDALDGL DRWPDKVRLM QRNWIGRSEG LLIRFALDPK TVPAGESELK
260 270 280 290 300
IFTTRPDTLF GAKFMAISAD HPLAQAAAAK NPELAAFIAE IKRIGTAQEA
310 320 330 340 350
IDTAEKQGFD TGIRAVHPFD PSWTLPVYVA NFVLMEYGTG AIFGCPAHDQ
360 370 380 390 400
RDLDFVNKYG LGNTPVVCPE GQDPASFVIT DTAYDGDGRM INSRFLDGMT
410 420 430 440 450
IAQAKDEVAK RLESEQRGGS PIGERQVNFR LRDWGISRQR YWGCPIPVIH
460 470 480 490 500
CATCDVVPVP AGDLPVVLPD DVTFDKPGNA LDHHPTWKHV TCPKCGGKAT
510 520 530 540 550
RETDTMDTFV DSSWYFARFT DPWNETAPTT PAVANRMMPV DQYIGGVEHA
560 570 580 590 600
ILHLLYSRFF TRAMKATGHV AMDEPFAGMF TQGMVVHETY QKADGTYVNP
610 620 630 640 650
ADVKIEVGGN GRRAVLTATG EDITIGPIEK MSKSKKNTVD PDDIIESYGA
660 670 680 690 700
DVARWFMLSD SPPDRDVIWS DERVQGAARF VQRLWRLVNE SVAASAEAPS
710 720 730 740 750
SRPANFSADA LALRKAAHGA LDKVTGGIER LHFNVCLAHI REFANALADV
760 770 780 790 800
LAKPAKPAPD LAWATREAAG ILVQLFSPMM PHLAEECWQV LGHSGLISEA
810 820 830 840 850
SWPQIERDLL VEDSMTLVVQ VNGKKRGEVT VASNAQNPEI ESAVLALDAV
860 870
KVALDGKPVR KVIIVPKRIV NVVG
Length:874
Mass (Da):97,349
Last modified:May 29, 2007 - v1
Checksum:iBE0C6EF60A3DCA47
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU234118 Genomic DNA. Translation: CAL74154.1.
RefSeqiWP_011923445.1. NC_009445.1.
YP_001202404.1. NC_009445.1.

Genome annotation databases

EnsemblBacteriaiCAL74154; CAL74154; BRADO0189.
KEGGibra:BRADO0189.
PATRICi21216702. VBIBraSp122330_0187.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU234118 Genomic DNA. Translation: CAL74154.1.
RefSeqiWP_011923445.1. NC_009445.1.
YP_001202404.1. NC_009445.1.

3D structure databases

ProteinModelPortaliA4YJS8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi114615.BRADO0189.

Proteomic databases

PRIDEiA4YJS8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL74154; CAL74154; BRADO0189.
KEGGibra:BRADO0189.
PATRICi21216702. VBIBraSp122330_0187.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciBSP114615:GJN5-183-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ORS278.

Entry informationi

Entry nameiSYL_BRASO
AccessioniPrimary (citable) accession number: A4YJS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 29, 2007
Last modified: April 1, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.